Distribution of Heavy Metals in Sediments and Soft Tissues of the Cerithidea obtusa from Sepang River, Malaysia


Krishnan Kumar(1*), Elias Saion(2), Chee Kong Yap(3), Prakash Balu(4), Wan Hee Cheng(5), Mee Yoke Chong(6)

(1) Faculty of Health and Life Sciences, Inti International University, Persiaran Perdana BBN, Nilai 71800, Negeri Sembilan, Malaysia
(2) Department of Physics, Faculty of Science, University Putra Malaysia, Serdang 43400, Selangor, Malaysia
(3) Department of Biology, Faculty of Science, University Putra Malaysia, Serdang 43400, Selangor, Malaysia
(4) Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), PV Vaithiyalingam Rd, Velan Nagar, Krishnapuram, Pallavaram, Chennai, Tamil Nadu 600117, India
(5) Faculty of Health and Life Sciences, Inti International University, Persiaran Perdana BBN, Nilai 71800, Negeri Sembilan, Malaysia
(6) Matrix Global School, Pt 12652, Sendayan Merchant Square, Persiaran 1 Sendayan Utama, Pusat Dagangan Sendayan, 71950 Bandar Sri Sendayan, Seremban, Negeri Sembilan, Malaysia
(*) Corresponding Author


The main purpose of the research was to analyze the distribution of Arsenic (As), Cadmium (Cd), Copper (Cu), Iron (Fe), Nickel (Ni), Cobalt (Co), Mangan (Mn), and Zinc (Zn) in soft tissues, shells, and associated surface sediments of Cerithidea obtusa (C. obtusa) mangrove snails collected from Sungai Besar Sepang. The concentration of iron (Fe) was found to be the highest in relation to other toxic elements in sediments, soft tissues, and shells of C. obtusa. The concentrations of Cu and Zn in soft tissues of C. obtusa were found to exceed the concentrations in sediments, indicating bioaccumulation of these metals. Metal pollution was assessed with the Enrichment Factor (EF), Geoaccumulation Index (Igeo), and Pollution Factor (CF). EF, Igeo, and CF were 0.34 to 22.41, -3.37 to 2.65, and 0.14 to 9.42, respectively. The results indicate that sediments in Sungai Besar Sepang are contaminated with As and Zn. According to the bivalve bioaccumulation results, the soft tissues of C. obtusa act as a macro-concentrator for Cu and Zn. As a result, it is suggested that ongoing monitoring of releases of heavy metals from anthropogenic sources and stricter environmental protection measures should be implemented.


enrichment; bio-accumulation; trace elements; neutron activation analysis

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[1] Ratih, S.A., Pertiwi, M.P., and Rostikawati, R.T., 2021, Mollusk diversity in the intertidal zone of Menganti Beach, Kebumen, Central Java, Depik, 10 (1), 23–29.

[2] Triacha, Z.I.E.C., Pertiwi, M.P., and Rostikawati, R.T., 2021, Keanekaragaman echinodermata di pantai Cibuaya Ujung Genteng, Jawa Barat, Jurnal Ilmu Dasar, 22 (1), 9–18.

[3] Yap, C.K., Sharifinia, M., Cheng, W.H., Al-Shami, S.A., Wong, K.W., and Al-Mutairi, K.A.A., 2021, Commentary on the use of bivalve mollusks in monitoring metal pollution levels, Int. J. Environ. Res. Public Health, 18 (7), 3386.

[4] Telesca, L., Peck, L.S., Sanders, T., Thyrring, J., Sejr, M.K., and Harper, E.M., 2019, Biomineralization plasticity and environmental heterogeneity predict geographical resilience patterns of foundation species to future change, Global Change Biol., 25 (12), 4179–4193.

[5] Rosa, M., Ward, J.E., and Shumway, S.E., 2018, Selective capture and ingestion of particles by suspension-feeding bivalve mollusks, A review, J. Shellfish Res., 37 (4), 727–746.

[6] Yap, C.K., Cheng, W.H., Karami, A., and Ismail, A., 2016, Health risk assessments of heavy metal exposure via consumption of marine mussels collected from anthropogenic sites, Sci. Total Environ., 553, 285–296.

[7] Yap, C.K., Chew, W., Cheng, H., Okamura, H., Harino, H., Peng, S.H.T., Ismail, M.S., and Leow, C.S., 2019, Higher bioavailability and contamination by copper in the edible mussels, snails and horseshoe crabs at Kampung Pasir Puteh: Evidence of an industrial effluent receiving site at Pasir Gudang area, Adv. Bioequivalence Bioavailability, 2 (5), 000548.

[8] Yap, C.K., 2017, From mussel watch monitoring to health risk assessment: A public health concern, GSL J Public Health Epidemiol., 1, 103.

[9] Alkan, N., Alkan, A., Demirak, A., and Bahloul, M., 2020, Metals/metalloid in marine sediments, bioaccumulating in macroalgae and a mussel, Soil Sediment Contam., 29 (5), 569–594.

[10] Yap, C.K., Ariffin, N., Nulit, R., Ibrahim, M.H., Peng, S.H.T., Yap, C.W., and Ng, W.K., 2019, The potential of digestive caecum in mudflat snail Telescopium telescopium as a possible cadmium source for industrial application, EC Gastroenterol. Dig. Syst., 6 (9), 752–756.

[11] Yasin, M.Y., Noor, N.M., Yusoff, M.M., Abdullah, J., and Noor, N.M., 2021, SPOT imagery observation on mangrove changes using NDVI density analysis: The case of Sepang Besar River, Malaysia, Arab World Geogr., 23 (2-3), 217–228.

[12] Yasin, M.Y., Yusoff, M.M., and Noor, N.M., 2019, Urban sprawl assessment using time series LULC and NDVI variation: A case study of Sepang Malaysia, Appl. Ecol. Environ. Res., 17 (3), 5583–5602.

[13] Yap, C.K., 2018, Selected organs of marine mussels as accurate biomonitors of metal bioavailability and contamination in the coastal waters: Challenges, EC Pharmacol. Toxicol., 6 (7), 528–534.

[14] Liu, J., Wu, H., Feng, J., Li, Z., and Lin, G., 2014, Heavy metal contamination and ecological risk assessments in the sediments and zoobenthos of selected mangrove ecosystems, South China, CATENA, 119, 136–142.

[15] Asimiea, O.A., and Gobo, A.E., 2012, Nematod speciation along the New Calabar and Bonny River systems of the Niger Delta, Nigeria, J. Emerging Trends Eng. Appl. Sci., 3 (5), 765–769.

[16] Ra, K., Kim, J.K., Hong, S.H., Yim, U.H., Shim, W.J., Lee, S.Y., Kim, Y.O., Lim, J., Kim, E.S., and Kim, K.T., 2014, Assessment of pollution and ecological risk of heavy metals in the surface sediments of Ulsan Bay, Korea, Ocean Sci. J., 49 (3), 279–289.

[17] Liu, Q., Liao, Y., and Shou, L., 2018, Concentration and potential health risk of heavy metals in seafoods collected from Sanmen Bay and its adjacent areas, China, Mar. Pollut. Bull., 131 (Pt A), 356–364.

[18] Krishnan, K., Saion, E.B., Yap, C.K., Chong, M.Y., and Nadia, A. S, 2022, Determination of trace elements in sediments samples by using neutron activation analysis, J. Exp. Biol. Agric. Sci., 10 (1), 21–31.

[19] Kumar, K., Saion, E., Halimah, M.K., Yap, C., and Hamzah, M.S., 2014, Rare earth element (REE) in surface mangrove sediment by instrumental neutron activation analysis, J. Radioanal. Nucl. Chem., 301 (3), 667–676.

[20] Benarfa, A., Begaa, S., Messaoudi, M., Hamlat, N., and Sawicka, B., 2020, Elemental composition analysis of Pistacia lentiscus L., leaves collected from Mitidja plain in Algeria using instrumental neutron activation analysis (INAA) technique, Radiochim. Acta, 108 (10), 821–828.

[21] Mir, S.I., Karim, M.A., Ali, M.I., and Ramli, N.I., 2017, Assessment of heavy metal contents in surface sediment of the Tungguk River surrounding the industrial complex of Gebeng City, BRC, 3 (1), 362–371.

[22] Khodami, S., Surif, M., Wan Omar, W.M., and Daryanabard, R., 2017, Assessment of heavy metal pollution in surface sediments of the Bayan Lepas area, Penang, Malaysia, Mar. Pollut. Bull., 114 (15), 615–622.

[23] Barbieri, M., 2016, The importance of enrichment factor (EF) and geoaccumulation index (Igeo) to evaluate the soil contamination, J. Geol. Geophys., 5 (1), 1000237.

[24] Müller, G., 1979, Schwermetalle in den sedimenten des Rheins: Veranderungen seitt 1971, Umschau, 79, 778–783.

[25] Turekian, K.K., and Wedepohl, K.H., 1961, Distribution of the elements in some major units of the earth’s crust, Geol. Soc. Am. Bull., 72 (2), 175–192.

[26] Müller, G., 1981, Die schwermetallbelastung der sedimente des neckars und seiner nebenflusse: Eine bestandsaufnahme, Chem.-Ztg., 105, 157–164.

[27] Hakanson, L., 1980, An ecological risk index for aquatic pollution control. A sedimentological approach, Water Res., 14 (8), 975–1001.

[28] Kobkeatthawin, T., Sirivithayapakorn, S., Nitiratsuwan, T., Muenhor, D., Loh, P.S., and Pradit, S., 2021, Accumulation of trace metal in sediment and soft tissue of Strombus canarium in a tropical remote island of Thailand, J. Mar. Sci. Eng., 9 (9), 991.

[29] Yunus, K., Zuraidah, M.A., and John, A, 2020, A review on the accumulation of heavy metals in coastal sediment of Peninsular Malaysia, Ecofeminism Clim. Change, 1 (1), 21–35.

[30] Nourozifard, P., Mortazavi, S., Asad, S., and Hassanzadeh, N., 2020, Using Saccostrea cucullata as a biomonitor of heavy metals (Cu, Pb, Zn, Cd, Ni, and Cr) in water and sediments of Qeshm Island, Persian Gulf, ECOPERSIA, 8 (3), 181–190.

[31] Zulkifli, S.Z., Mohamat-Yusuff, F., Arai, T., Ismail, A., and Miyazaki, N, 2010, An assessment of selected trace elements in intertidal sediments collected from the Peninsular Malaysia, Environ. Monit. Assess., 169 (1), 457–472.

[32] Syaizwan, Z.Z., Siti, A.R., Ferdaus, M.y., and Ahmad, I., 2014, Geochemical fractionations of heavy metals in sediments of Sepang Besar River, Malaysia, Acta Biol. Malays., 3 (1), 1–9.

[33] Yap, C.K., Mohd Ruszaidi, S., Cheng, W.H., and Tan, S.G, 2010, Heavy metal concentrations in the mangrove snail, Nerita lineata and surface sediments collected from Klang River Estuary, Selangor, Malaysia, J. Sustainability Sci. Manage., 5 (1), 1–12.

[34] Abbasi, S., Soltani, N., Keshavarzi, B., Moore, F., Turner, A., and Hassanaghaei, M., 2018, Microplastics in different tissues of fish and prawn from the Musa Estuary, Persian Gulf, Chemosphere, 205, 80–87.

[35] Larsson, J., Smolarz, K., Świeżak, J., Turower, M., Czerniawska, N., and Grahn, M., 2018, Multibiomarker analysis of pollution effect on resident populations of blue mussels from the Baltic Sea, Aquat. Toxicol., 198, 240–256.

[36] Vernon, E.L., and Jha, A.N., 2019, Assessing relative sensitivities of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers, Mutat. Res., Genet. Toxicol. Environ. Mutagen., 842, 60–71.

[37] Chen, X., Wang, Z., Zhu, G., Nordberg, G.F., Ding, X., and Jin, T., 2018, The Association between renal tubular dysfunction and zinc level in a Chinese population environmentally exposed to cadmium, Biol. Trace Elem. Res., 186 (1), 114–186.

[38] Hertika, A.M.S., Kusriani, K., Indrayani, E., Yona, D., and Putra, R.B.D.S., 2019, Metallothionein expression on oysters (Crassostrea cuculata and Crassostrea glomerata) from the southern coastal region of East Java, F1000Research, 8, 56.

[39] Savoca, D., and Pace, A., 2021, Bioaccumulation, biodistribution, toxicology and biomonitoring of organofluorine compounds in aquatic organisms, Int. J. Mol. Sci., 22 (12), 6276.

[40] Yap, C.K., and Cheng, W.H., 2013, Distributions of heavy metal concentrations in different tissues of the mangrove snail Nerita lineata, Sains Malays., 42 (5), 597–603.

[41] Samara, F., Elsayed, Y., Soghomonian, B., and Knuteson, S.L., 2016, Chemical and biological assessment of sediments and water of Khalid Khor, Sharjah, United Arab Emirates, Mar. Pollut. Bull., 111 (1-2), 268–276.

[42] Ramsie, S.A., Zulkifli, S.Z., Mohamat-Yusuff, F., and Ismail, A., 2014, Geochemical fractionations of heavy metals in sediments of Sepang Besar River, Malaysia, Acta Biol. Malays., 3 (1), 1–9.

[43] Sundaray, S.K., Nayak, B.B., Lin, S., and Bhatta, D., 2011, Geochemical speciation and risk assessment of heavy metals in the river estuarine sediments‐A case study: Mahanadi basin, J. Hazard. Mater., 186 (2‐3), 1837–1846.

[44] Long, E.R., MacDonald, D.D., Smith, S.L., and Calder, F.D., 1995, Incidence of adverse biological effects within ranges of chemical concentrations in marine and estuarine sediments, Environ. Manage., 19 (1), 81–97.

DOI: https://doi.org/10.22146/ijc.72991

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