Strategies in Improving Sensitivity of Colorimetry Sensor Based on Silver Nanoparticles in Chemical and Biological Samples

Hanim Istatik Badi'ah(1), Dinda Khoirul Ummah(2), Ni Nyoman Tri Puspaningsih(3), Ganden Supriyanto(4*)

(1) Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Kampus C Mulyorejo, Surabaya 60115, Indonesia; Department of Medical Laboratory Technology, Institute of Health Science Banyuwangi, Jl. Letkol Istiqlah No. 109, Banyuwangi 68422, Indonesia
(2) Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Kampus C Mulyorejo, Surabaya 60115, Indonesia
(3) Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Kampus C Mulyorejo, Surabaya 60115, Indonesia
(4) Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Kampus C Mulyorejo, Surabaya 60115, Indonesia
(*) Corresponding Author


Colorimetric sensors-based silver nanoparticles (AgNPs) are very interesting to be studied and developed because of the simplicity and ease in the principle of detection. It does not require sophisticated and affordable tools but still has high sensitivity. The coefficient extinction of AgNPs is relatively higher than AuNPs of the same size, making the sensitivity of AgNPs higher than AuNPs. The principle of detection is based on the aggregation of nanoparticles with analytes that causes shifting in Localized Surface Plasmon Resonance (LSPR) to a larger wavelength, commonly called a bathochromic shift or redshift. It is a favorite phenomenon because it is more easily observed with naked eyes. This sensor shows a good analytic performance with high sensitivity due to strong LSPR and good strategies that selectively bring interaction between analytes and AgNPs. AgNPs are characterized using UV-Visible (Ultra Violet-Visible), TEM (Transmission Electron Microscope), FTIR (Fourier Transform Infrared), and DLS (Dynamic Light Scattering), and many analytes have been detected with this sensor successfully. This article discusses several important parameters in increasing the sensitivity of AgNPs colorimetric sensors. Finally, it can be used as guidelines in the development of methods in the future.


sensitivity; colorimetric sensor; AgNPs; aggregation; bathochromic

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[1] Shrivas, K., Sahu, J., Maji, P., and Sinha, D., 2017, Label-free selective detection of ampicillin drug in human urine samples using silver nanoparticles as a colorimetric sensing probe, New J. Chem., 41 (14), 6685–6692.

[2] Mohammadi, S., and Khayatian, G., 2017, Colorimetric detection of biothiols based on aggregation of chitosan-stabilized silver nanoparticles, Spectrochim. Acta, Part A, 185, 27–34.

[3] Li, N., Gu, Y., Gao, M., Wang, Z., Xiao, D., Li, Y., Wang, J., and He, H., 2014, Label-free silver nanoparticles for visual colorimetric detection of etimicin, Anal. Methods, 6 (19), 7906–7911.

[4] Chen, S., Gao, H., Shen, W., Lu, C., and Yuan, Q., 2013, Colorimetric Colorimetric detection of cysteine using noncrosslinking aggregation of fluorosurfactant-capped silver nanoparticles, Sens. Actuators, B, 190, 673–678.

[5] Alula, M.T., Karamchand, L., Hendricks, N.R., and Blackburn, J.M., 2018, Citrate-capped silver nanoparticles as a probe for sensitive and selective colorimetric and spectrophotometric sensing of creatinine in human urine, Anal. Chim. Acta, 1007, 40–49.

[6] Thomas, A., Sivasankaran, U., and Kumar, K.G., 2018, Biothiols induced colour change of silver nanoparticles: A colorimetric sensing strategy, Spectrochim. Acta, Part A, 188, 113–119.

[7] Chavada, V.D., Bhatt, N.M., Sanyal, M., and Shrivastav, P.S., 2017, Surface plasmon resonance based selective and sensitive colorimetric determination of azithromycin using unmodified silver nanoparticles in pharmaceuticals and human plasma, Spectrochim. Acta, Part A, 170, 97–103.

[8] Badi’ah, H.I., 2018, Nanopartikel Perak Termodifikasi Kitosan sebagai Sensor Kolorimetri Asam Sialat, Thesis, Universitas Airlangga, Indonesia.

[9] Kappi, F.A., Tsogas, G.Z., Giokas, D.L., Christodouleas, D.C., and Vlessidis, A.G., 2014, Colorimetric and visual read-out determination of cyanuric acid exploiting the interaction between melamine and silver nanoparticles, Microchim. Acta, 181 (5), 623–629.

[10] Ma, Y., Niu, H., Zhang, X., and Cai, Y., 2011, Colorimetric detection of copper ions in tap water during the synthesis of silver/dopamine nanoparticles, Chem. Commun., 47 (47), 12643–12645.

[11] Rohit, J.V., Solanki, J.N., and Kailasa, S.K., 2014, Surface modification of silver nanoparticles with dopamine dithiocarbamate for selective colorimetric sensing of mancozeb in environmental samples, Sens. Actuators, B, 200, 219–226.

[12] Xiong, D., and Li, H., 2008, Colorimetric detection of pesticides based on calixarene modified silver nanoparticles in water, Nanotechnology, 19 (46), 465502.

[13] Wang, G.L., Zhu, X.Y., Jiao, H.J., Dong, Y.M., Wu, X.M., and Li, Z.J., 2012, “Oxidative etching-aggregation” of silver nanoparticles by melamine and electron acceptors: An innovative route toward ultrasensitive and versatile functional colorimetric sensors, Anal. Chim. Acta, 747, 92–98.

[14] Nafia, I., 2012, Nanopartikel Perak Termodifikasi L-Sistein sebagai Indikator Warna untuk Logam Pencemar pada Sampel Ikan Tongkol, Undergraduate Thesis, Universitas Indonesia, Indonesia.

[15] Song, J., Wu, F., Wan, Y., and Ma, L.H., 2014, Visual test for melamine using silver nanoparticles modified with chromotropic acid, Microchim. Acta, 181 (11), 1267–1274.

[16] Seedeh, F., 2018, Modifikasi Nanopartikel Perak dengan Sisteamin sebagai Sensor Melamin secara Kolorimetri, Thesis, Universitas Airlangga, Indonesia.

[17] Amirjani, A., Bagheri, M., Heydari, M., and Hesaraki, S., 2016, Colorimetric determination of Timolol concentration based on localized surface plasmon resonance of silver nanoparticles, Nanotechnology, 27 (37), 375503.

[18] Blake-Hedges, J.M., Greenspan, S.H., Kean, J.A., McCarron, M.A., Mendonca, M.L., and Wustholz, K.L., 2015, Plasmon-enhanced fluorescence of dyes on silica-coated silver nanoparticles: A single-nanoparticle spectroscopy study, Chem. Phys. Lett., 635, 328–333.

[19] Jinnarak, A., and Teerasong, S., 2016, A novel colorimetric method for detection of gamma-aminobutyric acid based on silver nanoparticles, Sens. Actuators, B, 229, 315–320.

[20] Kailasa, S.K., Koduru, J.R., Desai, M.L., Park, T.J., Singhal, R.K., and Basu, H., 2018, Recent progress on surface chemistry of plasmonic metal nanoparticles for colorimetric assay of drugs in pharmaceutical and biological samples, TrAC, Trends Anal. Chem., 105, 106–120.

[21] Laliwala, S.K., Mehta, V.N., Rohit, J.V., and Kailasa, S.K., 2014, Citrate-modified silver nanoparticles as a colorimetric probe for simultaneous detection of four triptan-family drugs, Sens. Actuators, B, 197, 254–263.

[22] Rostami, S., Mehdinia, A., and Jabbari, A., 2017, Seed-mediated grown silver nanoparticles as a colorimetric sensor for detection of ascorbic acid, Spectrochim. Acta, Part A, 180, 204–210.

[23] Velugula, K., and Chinta, J.P., 2017, Silver nanoparticles ensemble with Zn(II) complex of terpyridine as a highly sensitive colorimetric assay for the detection of Arginine, Biosens. Bioelectron., 87, 271–277.

[24] Li, C., and Wei, C., 2017, DNA-templated silver nanocluster as a label-free fluorescent probe for the highly sensitive and selective detection of mercury ions, Sens. Actuators, B, 242, 563–68.

[25] de Oliveira, D.P., and de Siqueira, M.E.P.B., 2007, A simple and rapid method for urinary acetone analysis by headspace/gas chromatography, Quim. Nova, 30 (5), 1362–1364.

[26] Teerlink, T., 2007, HPLC analysis of ADMA and other methylated L-arginine analogs in biological fluids, J. Chromatogr. B, 851 (1-2), 21–29.

[27] Tebani, A., Schlemmer, D., Imbard, A., Rigal, O., Porquet, D., and Benoist, J.F., 2011, Measurement of free and total sialic acid by isotopic dilution liquid chromatography tandem mass spectrometry method, J. Chromatogr. B, 879 (31), 3694–3699.

[28] Shimelis, O., Santasania, C.T., and Trinh, A., 2009, The Extraction and Analysis of Melamine in Milk-Based Products Using Discovery DSC-SCX SPE and Ascentis Express HILIC LC-MS/MS, Technical Report, Sigma-Aldrich, Bellefonte, USA.

[29] Saha, K., Agasti, S.S., Kim, C., Li, X., and Rotello, V.M., 2012, Gold nanoparticles in chemical and biological sensing, Chem. Rev., 112 (5), 2739–2779.

[30] Tan, W., Zhang, L., Doery, J.C.G., and Shen, W., 2020, Study of paper-based assaying system for diagnosis of total serum bilirubin by colorimetric diazotization method, Sens. Actuators, B, 305, 127448.

[31] Cui, X., Wei, T., Hao, M., Qi, Q., Wang, H., and Dai, Z., 2020, Highly sensitive and selective colorimetric sensor for thiocyanate based on electrochemical oxidation-assisted complexation reaction with gold nanostars etching, J. Hazard. Mater., 391, 122217.

[32] Navarro, J., de Marcos, S., and Galbán, J., 2020, Colorimetric-enzymatic determination of tyramine by generation of gold nanoparticles, Microchim. Acta, 187 (3), 174.

[33] Keshvari, F., Bahram, M., and Farhadi, K., 2016, Sensitive and selective colorimetric sensing of acetone based on gold nanoparticles capped with L-cysteine, J. Iran. Chem. Soc., 13 (8), 1411–1416.

[34] Plata, M.R., Contento, A.M., and Ríos, A., 2010, State-of-the-art of (bio)chemical sensor developments in analytical Spanish groups, Sensors, 10 (4), 2511–2576.

[35] Zhou, W., Gao, X., Liu, D., and Chen, X., 2015, Gold nanoparticles for in vitro diagnostics, Chem. Rev., 115 (19), 10575–10636.

[36] Oliveira, E., Núñez, C., Santos, H.M., Fernández-Lodeiro, J., Fernández-Lodeiro, A., Capelo, J.L., and Lodeiro, C., 2015, Revisiting the use of gold and silver functionalised nanoparticles as colorimetric and fluorometric chemosensors for metal ions, Sens. Actuators, B, 212, 297–328.

[37] Baptista, P., Pereira, E., Eaton, P., Doria, G., Miranda, A., Gomes, I., Quaresma, P., and Franco, R., 2008, Gold nanoparticles for the development of clinical diagnosis methods, Anal. Bioanal. Chem., 391 (3), 943–950.

[38] Lee, K.S., and El-Sayed, M.A., 2006, Gold and silver nanoparticles in sensing and imaging: Sensitivity of plasmon response to size, shape, and metal composition, J. Phys. Chem. B, 110 (39), 19220–19225.

[39] Sun, Y., and Xia, Y., 2003, Gold and silver nanoparticles: A class of chromophores with colors tunable in the range from 400 to 750 nm, Analyst, 128 (6), 686–691.

[40] Zhang, Z., Wang, H., Chen, Z., Wang, X., Choo, J., and Chen, L., 2018, Plasmonic colorimetric sensors based on etching and growth of noble metal nanoparticles: Strategies and applications, Biosens. Bioelectron., 114, 52–65.

[41] D’souza, S.L., Pati, R., and Kailasa, S.K., 2015, Ascorbic acid-functionalized Ag NPs as a probe for colorimetric sensing of glutathione, Appl. Nanosci., 5 (6), 747–753.

[42] Nsengiyuma, G., Hu, R., Li, J., Li, H., and Tian, D., 2016, Self-assembly of 1,3-alternate calix[4]arene carboxyl acids-modified silver nanoparticles for colorimetric Cu2+ sensing, Sens. Actuators, B, 236, 675–681.

[43] Jazayeri, M.H., Aghaie, T., Avan, A., Vatankhah, A., and Ghaffari, M.R.S., 2018, Colorimetric detection based on gold nano particles (GNPs): An easy, fast, inexpensive, low-cost and short time method in detection of analytes (protein, DNA, and ion), Sens. Bio-Sens. Res., 20, 1–8.

[44] Ghosh, S.K., and Pal, T., 2007, Interparticle coupling effect on the surface plasmon resonance of gold nanoparticles: From theory to applications, Chem. Rev., 107 (11), 4797–4862.

[45] Hu, R., Long, G., Chen, J., Yin, Y., Liu, Y., Zhu, F., Feng, J., Mei, Y., Wang, R., Xue, H., Tian, D., and Li, H., 2015, Highly sensitive colorimetric sensor for the detection of H2PO4− based on self-assembly of p-sulfonatocalix[6]arene modified silver nanoparticles, Sens. Actuators, B, 218, 191–195.

[46] Mulfinger, L., Solomon, S.D., Bahadory, M., Jeyarajasingam, A.V., Rutkowsky, S.A., and Boritz, C., 2007, Synthesis and study of silver nanoparticles, J. Chem. Educ., 84 (2), 322.

[47] Ramalingam, K., Devasena, T., Senthil, B., Kalpana, R., and Jayavel, R., 2017, Silver nanoparticles for melamine detection in milk based on transmitted light intensity, IET Sci., Meas. Technol., 11 (2), 171–178.

[48] Badi'ah, H.I., Seedeh, F., Supriyanto, G., and Zaidan, A.H., 2019, Synthesis of silver nanoparticles and the development in analysis method, IOP Conf. Ser.: Earth Environ. Sci., 217, 012005.

[49] Irwan, R., Zakir, M., and Budi, P., 2020, Sintesis nanopartikel perak dan pengaruh penambahan asam p-kumarat untuk aplikasi deteksi melamin, Indo. J. Chem. Res., 7 (2), 141–150.

[50] Rawat, K.A., Singhal, R.K., and Kailasa, S.K., 2017, One-pot synthesis of silver nanoparticles using folic acid as a reagent for colorimetric and fluorimetric detections of 6-mercaptopurine at nanomolar concentration, Sens. Actuators, B, 249, 30–38.

[51] Badi'ah, H.I., 2021, Chitosan as capping agent for silver nanoparticles, Indo. J. Chem. Res., 9 (9), 21–25.

[52] Balasurya, S., Syed, A., Thomas, A.M., Bahkali, A.H., Elgorban, A.M., Raju, L.L., and Khan, S.S., 2020, Highly sensitive and selective colorimetric detection of arginine by polyvinylpyrrolidone functionalized silver nanoparticles, J. Mol. Liq., 300, 112361.

[53] Li, H., Zhang, L., Yao, Y., Han, C., and Jin, S., 2010, Synthesis of aza-crown ether-modified silver nanoparticles as colorimetric sensors for Ba2+, Supramol. Chem., 22 (9), 544–547.

[54] Inamuddin, I., and Kanchi, S., 2020, One-pot biosynthesis of silver nanoparticle using Colocasia esculenta extract: Colorimetric detection of melamine in biological samples, J. Photochem. Photobiol., A, 391, 112310.

[55] Fu, L.M., Hsu, J.H., Shih, M.K., Hsieh, C.W., Ju, W.J., Chen, Y.W., Lee, B.H., and Hou, C.Y., 2021, Process optimization of silver nanoparticles synthesis and its application in mercury detection, Micromachines, 12 (9), 1123.

[56] Che Sulaiman, I.S., Chieng, B.W., Osman, M.J., Ong, K.K., Rashid, J.I.A., Wan Yunus, W.M.Z., Noor, S.A.M., Kasim, N.A.M., Halim, N.A., and Mohamad, A., 2020, A review on colorimetric methods for determination of organophosphate pesticides using gold and silver nanoparticles, Microchim. Acta, 187 (2), 131.

[57] Taverniers, I., De Loose, M., and Van Bockstaele, E., 2004, Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance, TrAC, Trends Anal. Chem., 23 (8), 535–552.

[58] Harmita, 2004, Petunjuk pelaksanaan validasi dan cara perhitungannya, MIK, 1 (3),117–35.

[59] Patel, J., Patil, S., and Pawar, S., 2017, A review on method development and validation, World J. Pharm. Pharm. Sci., 6 (3), 245–259.

[60] Ringe, E., Sharma, B., Henry, A.I., Marks, L.D., and Van Duyne, R.P., 2013, Single nanoparticle plasmonic, Phys. Chem. Chem. Phys., 15 (12), 4110–4129.

[61] Jouyban, A., and Rahimpour, E., 2020, Optical sensors based on silver nanoparticles for determination of pharmaceuticals: An overview of advances in the decade, Talanta, 217, 121071.

[62] Mohsen, E., El-Borady, O.M., Mohamed, M.B., and Fahim, I.S., 2020, Synthesis and characterization of ciprofloxacin loaded silver nanoparticles and investigation of their antibacterial effect, J. Radiat. Res. Appl. Sci., 13 (1), 416–425.

[63] Erjaee, H., Rajaian, H., and Nazifi, S., 2017, Synthesis and characterization of novel silver nanoparticles using Chamaemelum nobile extract for antibacterial application, Adv. Nat. Sci: Nanosci. Nanotechnol., 8 (2), 025004.

[64] Üzer, A., Durmazel, S., Erçağ, E., and Apak, R., 2017, Determination of hydrogen peroxide and triacetone triperoxide (TATP) with a silver nanoparticles—based turn-on colorimetric sensor, Sens. Actuators, B, 247, 98–107.

[65] Lin, Y., Chen, C., Wang, C., Pu, F., Ren, J., and Qu, X., 2011, Silver nanoprobe for sensitive and selective colorimetric detection of dopaminevia robust Ag–catechol interaction, Chem. Commun., 47 (4), 1181–1183.

[66] Zhan, J., Wen, L., Miao, F., Tian, D., Zhu, X., and Li, H., 2012, Synthesis of a pyridyl-appended calix[4]arene and its application to the modification of silver nanoparticles as an Fe3+ colorimetric sensor, New J. Chem., 36 (3), 656–661.

[67] Hoang, V.T., Ngo, X.D., Le Nhat Trang, N., Thi Nguyet Nga, D., Khi, N.T., Trang, V.T., Lam, V.D., and Le, A.T., 2022, Highly selective recognition of acrulamide in food samples using colorimetric sensor based on electrochemically synthesized colloidal silver nanoparticles: Role of supporting agent on cross-linking aggregation, Colloids Surf., A, 636, 128165.

[68] Su, Y.C., Lin, A.Y., Hu, C.C., and Chiu, T.C., 2021, Functionalized silver nanoparticles as colorimetric probes for sensing tricylazole, Food Chem., 347, 129044.

[69] Puente, C., Gómez, I., Kharisov, B., and López, I., 2018, Selective colorimetric sensing of Zn(II) ions using green-synthesized silver nanoparticles: Ficus benjamina extract as reducing and stabilizing agent, Mater. Res. Bull., 112, 1–8.

[70] Sadeghi, S., and Hosseinpour-Zaryabi, M., 2020, Sodium gluconate capped silver nanoparticles as a highly sensitive and selective colorimetric probe for the naked eye sensing of creatinine in human serum and urine, Microchem. J., 154, 104601.


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