Synthesis Optimization and Antibacterial Performance of Colloidal Silver Nanoparticles in Chitosan

Endang Susilowati(1*), Lina Mahardiani(2), Sri Retno Dwi Ariani(3), Ilham Maulana Sulaeman(4)

(1) Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta 57126, Indonesia
(2) Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta 57126, Indonesia
(3) Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta 57126, Indonesia
(4) Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta 57126, Indonesia
(*) Corresponding Author


Colloidal silver nanoparticles were successfully synthesized via the chemical reduction method. The synthesis used AgNO3 as the precursor, chitosan as the reducing and stabilizing agents, and NaOH as the accelerator. The synthesis parameters were optimized. The samples were tested with a UV-vis spectrophotometer to observe their localized surface plasmon resonance (LSPR) phenomenon, a transmission electron microscope (TEM), and a particle size analyzer (PSA) to investigate their particle shape and size distribution. Further, silver nanoparticles were tested for their storage stability and antibacterial performance. The UV-vis spectroscopy data exhibited that the silver nanoparticles have been successfully synthesized, validating via the emergence of the LSPR absorption band at 402–418 nm. At 50 °C, the optimum synthesis was achieved for 100 min of reaction time by adding 0.033 M NaOH and AgNO3 4.00% (w/w, AgNO3/chitosan). TEM results showed spherical silver nanoparticles of 1–8 nm, while the PSA results exhibited particles sizes of about 12–59 nm. The colloidal silver nanoparticles were stable in storage for 8 weeks and had good antibacterial performance against E. coli, S. aureus, extended-spectrum beta-lactamases (ESBL), and methicillin-resistant S. aureus (MRSA). Therefore, colloidal silver nanoparticles have the potential as a material for medical applications.


antibacterial performance; colloidal silver nanoparticles; chitosan; synthesis optimization

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[1] Iber, B.T., Kasan, A., Torsabo, D., and Omuwa, J.W., 2022, A review of various sources of chitin and chitosan in nature, J. Renewable Mater., 10 (4), 1097–1123.

[2] Badawy, M.E.I., and Rabea, E.I.A., 2011, A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection, Int. J. Carbohydr. Chem., 2011, 460381.

[3] Ardean, C., Davidescu, C.M., Nemeş, N.S., Negrea, A., Ciopec, M., Duteanu, N., Negrea, P., Duda-Seiman, D., and Musta, V., 2021, Factors influencing the antibacterial activity of chitosan and chitosan modified by functionalization, Int. J. Mol. Sci., 22 (14), 7449.

[4] Valverde-Alva, M.A., García-Fernández, T., Villagrán-Muniz, M., Sánchez-Aké, C., Castañeda-Guzmán, R., Esparza-Alegría, E., Sánchez-Valdés, C.F., Llamazares, J.L.S., and Herrera, C.E.M., 2015, Synthesis of silver nanoparticles by laser ablation in ethanol: A pulsed photoacoustic study, Appl. Surf. Sci., 355, 341–349.

[5] Verma, A., and Mehata, M.S., 2015, Controllable synthesis of silver nanoparticles using neem leaves and their antimicrobial activity, J. Radiat. Res. Appl. Sci., 9 (1), 109–115.

[6] David, L., Moldovan, B., Vulcu, A., Olenic, L., Perde-Schrepler, M., Fischer-Fodor, E., Florea, A., Crisan, M., Chiorean, I., Clichici, S., and Filip, G.A., 2014, Green synthesis, characterization and anti-inflammatory activity of silver nanoparticles using European black elderberry fruits extract, Colloids Surf., B, 122, 767–777.

[7] Gholamali, I., Asnaashariisfahani, M., and Alipour, E., 2020, Silver nanoparticles incorporated in pH-sensitive nanocomposite hydrogels based on carboxymethyl chitosan-poly (vinyl alcohol) for use in a drug silver system, Regener. Eng. Transl. Med., 6 (2), 138–153.

[8] Guzmán, M.G., Dille, J., and Godet, S., 2009, Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity, Int. J. Chem. Biomol. Eng., 2 (3), 104–111.

[9] Iravani, S., Korbekandi, H., Mirmohammadi, S.V., and Zolfaghari, B., 2014, Synthesis of silver nanoparticles: Chemical, physical and biological methods, Res. Pharm. Sci., 9 (6), 385–406.

[10] Reddy, G., and Thakur, A., 2017, Biogenic synthesis of silver nanoparticles using plant waste material, Rasayan J. Chem., 10 (3), 695–699.

[11] Ibrahim, H.M.M., 2015, Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms, J. Radiat. Res. Appl. Sci., 8 (3), 265–275.

[12] Srirangam, G.M., and Rao, K.P., 2017, Synthesis and characterization of silver nanoparticles from the leaf extract of Malachra capitata (L.), Rasayan J. Chem., 10 (1), 46–53.

[13] Susilowati, E., Triyono, T., Santosa, S.J., and Kartini, I., 2015, Synthesis of silver-chitosan nanocomposites colloidal by glucose as reducing agent, Indones. J. Chem., 15 (1), 29–35.

[14] Susilowati, E., Masykuri, M., Ulfa, M., and Puspitasari, D., 2020, Preparation of silver-chitosan nanocomposites colloidal and film as antibacterial material, JKPK, 5 (3), 300–310.

[15] Wei, D., Sun, W., Qian, W., Ye, Y., and Ma, X., 2009, The synthesis of chitosan-based silver nanoparticles and their antibacterial activity, Carbohydr. Res., 344 (17), 2375–2382.

[16] Darroudi, M., Ahmad, M., Abdullah, A.H., and Ibrahim, N.A., and Shameli, K., 2010, Effect of accelerator in green synthesis of silver nanoparticles, Int. J. Mol. Sci., 11 (10), 3898–3905.

[17] Ardani, H.K., Imawan, C., Handayani, W., Djuhana, D., Harmoko, A., and Fauzia, V., 2017, Enhancement of the stability of silver nanoparticles synthesized using aqueous extract of Diospyros discolor Willd. leaves using polyvinyl alcohol, IOP Conf. Ser.: Mater. Sci. Eng., 188, 012056.

[18] Susilowati, E., Maryani, M., and Ashadi, A., 2019, Green synthesis of silver-chitosan nanocomposite and their application as antibacterial material, J. Phys.: Conf. Ser., 1153, 012135.

[19] Susilowati, E., Ariani, S.R.D., Mahardiani, L., and Izzati, L., 2021, Synthesis and characterization chitosan film with silver nanoparticles addition as a multiresistant antibacterial material, JKPK, 6 (3), 371–383.

[20] Jahangirian, H., Haron, M.J., Ismail, M.H.S., Rafiee-Moghaddam, R., Afsah-Hejri, L., Abdollahi, Y., and Vafaei, N., 2013, Well diffusion method for evaluation of antibacterial activity, Dig. J. Nanomater. Biostruct., 8 (3), 1263–1270.

[21] Pestov, A., Nazirov, A., Modin, E., Mironenko, A., and Bratskaya, S., 2015, Mechanism of Au(III) reduction by chitosan: Comprehensive study with 13C and 1H NMR analysis of chitosan degradation products, Carbohydr. Polym., 117, 70–77.

[22] Patra, J.K., and Baek, K.H., 2014, Green nanobiotechnology: Factors affecting synthesis and characterization techniques, J. Nanomater., 2014, 417305.

[23] Kalaivani, R., Maruthupandy, M., Muneeswaran, T., Hameedha Beevi, A., Anand, M., Ramakritinan, C.M., and Kumaraguru, A.K., 2018, Synthesis of chitosan mediated silver nanoparticles (AgNPs) for potential antimicrobial applications, Front. Lab. Med., 2 (1), 30–35.

[24] Maguire, C.M., Rösslein, M., Wick, P., and Prina-Mello, A., 2018, Characterisation of particles in solution – A perspective on light scattering and comparative technologies, Sci. Technol. Adv. Mater., 19 (1), 732–745.

[25] Danaei, M., Dehghankhold, M., Ataei, S., Davarani, F.H., Javanmard, R., Dokhani, A., Khorasani, S., and Mozafari, M.R., 2018, Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems, Pharmaceutics, 10 (2), 57.

[26] Regiel, A., Irusta, S., Kyzioł, A., Arruebo, M., and Santamaria, J., 2013, Preparation and characterization of chitosan-silver nanocomposite films and their antibacterial activity against staphylococcus aureus, Nanotechnology, 24, 015101.

[27] Marambio-Jones, C., and Hoek, E.M.V., 2010, A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment, J. Nanopart. Res., 12 (5), 1531–1551.


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