Spectrophotometric Determination of Sodium Sulfacetamide Using Pyrocatechol as an Oxidative Coupling Agent


Marib Ismail Ali(1), Israa Talib Humeidy(2*)

(1) Department of Chemical Engineering, College of Engineering, University of Tikrit, Tikrit 34001, Iraq
(2) Department of Chemical Engineering, College of Engineering, University of Tikrit, Tikrit 34001, Iraq
(*) Corresponding Author


Oxidative coupling reaction is one of the simplest and fastest spectrophotometric methods for measuring sodium sulfacetamide (SCS) in pure formulations and pharmaceutical formulations (eye ointment) when compared to other methods that require difficult and costly operating conditions. Here, a new approach for quantifying SCS drug spectrophotometrically by oxidative coupling was described. The research is based on the oxidation of the drug with potassium periodate and then conjugation with pyrocatechol reagent in the neutral medium, which results in a water-soluble and stable reddish-brown product that had an absorption band at 500.5 nm. In this work, oxidant agent quantity, coupling reagent quantity, oxidation time, temperature effect, stoichiometry between SCS and pyrocatechol, interference effect, and calibration curve were all studied. Beer's law linearity ranged from 6.25–112.5 µg mL−1. The molar absorptivity, Sandell’s index, detection limit, determination coefficient, and average recovery percentage were 5185.7 L mol−1 cm−1, 0.049 μg cm−2, 0.0889 µg mL−1, 0.9988, and 100.34% respectively. The determination of sulfacetamide in pharmaceutical preparation eye, ointment was successful using this method.


oxidative coupling; potassium periodate; pyrocatechol; sulfacetamide sodium

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[1] Sayed, R.A., Mohamed, A.R., Hassan, W.S., and Elmasry, M.S., 2022, Earth-friendly-assessed chromatographic platforms for rapid analysis of sulfacetamide sodium and prednisolone acetate in presence of sulfanilamide impurity: Application on ophthalmic formulation and aqueous humor, Sustainable Chem. Pharm., 27, 100694.

[2] Jeliński, T., Przybyłek, M., and Cysewski, P., 2019, Solubility advantage of sulfanilamide and sulfacetamide in natural deep eutectic systems: Experimental and theoretical investigations, Drug Dev. Ind. Pharm., 45 (7), 1120–1129.

[3] Alaallah, N.J., Dhahir, S.A., and Ali, H.H., 2021, Determination of sulfacetamide sodium in pure and their pharmaceutical formulations by using cloud point extraction method, Baghdad Sci. J., 18 (3), 575–582.

[4] Al-Safar, R.S., and Othman, N.S., 2020, Spectrophotometric determination of sulphacetamide sodium via diazotization and coupling reaction, IOP Conf. Ser.: Mater. Sci. Eng., 928 (5), 052017.

[5] British Pharmacopoeia Commission, 2009, British Pharmacopoeia 2009, 6th Ed., Stationary Office, London.

[6] Vislohuzova, T.V., Rozhnova, R.A., Galatenko, N.A., and Nechaeva, L.Y.U., 2022, Investigation of the ability to biodegradability of polyurethane foam composite materials with albucid and the dynamics of albucid release in vitro, Polym. J., 44 (2), 145–154.

[7] Vislohuzova, T., Rozhnova, R., and Galatenko, N., 2021, Development and research of polyurethane foam composite materials with albucid, Am. J. Polym. Sci. Technol., 7 (3), 38–43.

[8] Patel, S., and Baria, D., 2023, Validated thin-layer chromatography method for simultaneous determination of sulfacetamide sodium and hydrocortisone acetate in the ophthalmic formulation, Sep. Sci. Plus, 6 (2), 2200090.

[9] Nagpal, M.A., Sharma, K., Anand, N., Singh, D., Dhawan, R., Usman, M.R.M., and Nagpal, N., 2020, Preparation and evaluation of sulfacetamide sodium ocusert for controlled drug delivery, J. Drug Delivery Ther., 10 (2), 164–170.

[10] Darweesh, S.A., Al-Haidari, I.M.A., Mohammed, A.K., and Dikran, S.B., 2017, Spectrophotometric determinations of sulfacetamide following simple diazotization and coupling with chromotropic acid, Ibn Al-Haitham J. Pure Appl. Sci., 26 (3), 281–295.

[11] Talal, Z., and Bashir, W.A., 2019, Spectrophotometric Determination of Sulfacetamide, The Eurasia Proceedings of Science, Technology, Engineering & Mathematics (EPSTEM), 7, 385–390.

[12] Al-Uzri, W.A., and Fadil, G., 2017, Spectrophotometric determinations of sulfacetamide sodium in pharmaceutical preparation using 8-hydroxy-7-iodoquinoline-5-sulfonic acid as chromogenic reagent, Asian J. Chem., 29 (4), 782–786.

[13] Abdulrazzaq, T.M., and Sultan, S.H., 2023, Spectrophotometric determination of sulphacetamide sodium using 2,4-dinitrophenylhydrazine as coupling reagent, Coll. Basic Educ. Res. J., 19 (1), 741–752.

[14] Ahmed, S., Anwar, N., Sheraz, M.A., and Ahmad, I., 2017, Validation of a stability-indicating spectrometric method for the determination of sulfacetamide sodium in pure form and ophthalmic preparations J. Pharm. BioAllied Sci., 9 (2), 126–134.

[15] Bu, J., Deng, Z., Liu, H., Li, T., Yang, Y., and Zhong, S., 2021, The degradation of sulfamilamide wastewater by three-dimensional electrocatalytic oxidation system composed of activated carbon bimetallic particle electrode, J. Cleaner Prod., 324, 129256.

[16] Yadav, S.K., Choubey, P.K., Agrawal, B., and Goyal, R.N., 2014, Carbon nanotube embedded poly 1,5-diaminonapthalene modified pyrolytic graphite sensor for the determination of sulfacetamide in pharmaceutical formulations, Talanta, 118 (15), 96–103.

[17] Fu, L., Zhang, X., Ding, S., Chen, F., Lv, Y., Zhang, H., and Zhao, S., 2022, Recent developments in the electrochemical determination of sulfonamides, Curr. Pharm. Anal., 18 (1), 4–13.

[18] El-Ragehy, N.A., Hegazy, M.A., AbdElHamid, G., and Tawfik, S.A., 2018, Validated potentiometric method for the determination of sulfacetamide sodium; Application to its pharmaceutical formulations and spiked rabbit aqueous humor, Bull. Fac. Pharm., Cairo Univ., 56 (2), 207–212.

[19] Fares, N.V., Medhat, P.M., Ayad, M.F., and El Maraghy, C.M., 2023, Cobalt oxide nanoparticles modified coated graphite potentiometric sensor for quantification of sulfacetamide sodium in its pharmaceutical dosage form and spiked rabbit aqueous humor samples with greenness assessment, Microchem. J., 195, 109435.

[20] El-Ragehy, N.A., Hegazy, M.A., AbdElHamid, G., and Tawfik, S.A., 2017, Validated chromatographic methods for the simultaneous determination of sulfacetamide sodium and prednisolone acetate in their ophthalmic suspension, J. Chromatogr. Sci., 55 (10), 1000–1005.

[21] Nabiha, A., Fizza, K., Shaista, Q., Mateen, A., Abdul, M.K., and Naseem, U., 2019, Development and validation of RP-HPLC method for simultaneous quantification of sulfacetamide sodium and prednisolone sodium phosphate, Acta Poloniae Pharmaceutica, 76(1), 37-47.

[22] Wen, Y., Zhang, M., Zhao, Q., and Feng, Y.Q., 2005, Monitoring of five sulfonamide antibacterial residues in milk by in-tube solid-phase microextraction coupled to high-performance liquid chromatography, J. Agric. Food Chem., 53 (22), 8468–8473.

[23] El-Ragehy, N.A., Hegazy, A.M., Tawfik, S.A., and Sedik, G.A. 2021, Validated chromatographic methods for the simultaneous determination of a ternary mixture of sulfacetamide sodium and two of its official impurities; sulfanilamide and dapsone, Acta Chromatogr., 34 (4), 377–385.

[24] Rao, R.M., Rao, Y.M., and Shah, A.H., 1999, A reverse phase ion-pairing HPLC method for the stability monitoring of sulphacetamide ophthalmic preparations, J. Pharm. Biomed. Anal., 20 (4), 717–722.

[25] Khaleel, A.I., Amine, S.T., and Salih, S.H., 2013, Comparative study for determination of metoclopramide hydrochloride drug by selective electrodes and spectrophotometric methods, Tikrit J. Pure Sci., 18 (1), 149–161.

[26] Skoog, D.A., West, D.M., Holler, F.J., and Crouch, S.R., 2014, “Errors in Chemical Analyses” in Fundamentals of Analytical Chemistry, 9th Ed., Brooks/Cole, Belmont, CA, US, 82–91.

[27] Humeidy, I.T., 2021, Spectrophotometric determination of cefotaxime sodium in pharmaceutical formulations, Mater. Today: Proc., 47, 6043–6049.

[28] Humeidy, I.T., Salman, S.A., and Hashim, K.K., 2020, Spectrophotometric determination of methyldopa with 2,6-diaminopyridine reagent using oxidative coupling reaction, J. Eng. Sci. Technol., 15 (3), 1824–1839.

DOI: https://doi.org/10.22146/ijc.85846

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