Bioformation of N-Acetylglucosamine from Shrimp Shell Chitin by Serratia marcescens PT-6 Cultured in Various pH and Temperature

https://doi.org/10.22146/jfs.25961

Bekti Wulan Sari(1*), Nurul Binti Isnaini(2), Indun Dewi Puspita(3), Amir Husni(4), Ustadi Ustadi(5)

(1) Department of Fisheries, Faculty of Agriculture, UGM.
(2) Department of Fisheries, Faculty of Agriculture, UGM.
(3) Department of Fisheries, Faculty of Agriculture, UGM.
(4) Department of Fisheries, Faculty of Agriculture, UGM.
(5) Department of Fisheries, Faculty of Agriculture, UGM.
(*) Corresponding Author

Abstract


The study aimed to determine the effect of pH and temperature on N-Acetylglucosamine (GlcNAc) formation from shrimp shell chitin by Serratia marcescens PT-6 fermented in chitin broth medium. Parameters examined on GlcNAc formation include bacteria growth, chitinase activity (U/ml), and GlcNAc concentration in medium (mg/ ml). Bacteria growth was measured by turbidity (OD600), while chitinase activity and GlcNAc concentration in the medium were analyzed quantitatively by colorimetric assay. The variation of initial pH examined in chitin broth medium was 5; 6; 7; 8, while temperature variation was 30°C; 37°C; dan 40°C. The results show that maximum concentration of GlcNAc formed by Serratia marcescens PT-6 was 39.30 mg/ml on day 5 of fermentation, at pH 7 and temperature of 30°C. Chitinase activity on the same day was 0.007 U/ml, while OD600 value of the culture was 0.51 indicating the bacteria were in the log phase. This research implies that Serratia marcescens PT-6 is potential to be optimized further for the bioconversion process of GlcNAc from shrimp shell waste through an enzymatic method by modifying its growth environment.

Keywords


Chitinase; chitin; N-Acetylglucosamine; Serratia marcescens PT-6

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DOI: https://doi.org/10.22146/jfs.25961

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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