PROFIL HETEROGENITAS GENETIK INDUK UDANG WINDU (Penaeus monodon) TURUNAN F1 MELALUI ANALISIS DNA MITOKONDRIA-RFLP DAN RAPD

https://doi.org/10.22146/jfs.2968

Bambang W. Prastowo(1*), Rahayu Rahardianti(2), Evi Maftuti Nur(3), Arief Taslihan(4)

(1) Balai Besar Pengembangan Budidaya Air Payau Jepara
(2) Balai Besar Pengembangan Budidaya Air Payau Jepara
(3) Balai Besar Pengembangan Budidaya Air Payau Jepara
(4) Balai Besar Pengembangan Budidaya Air Payau Jepara
(*) Corresponding Author

Abstract


Production of high quality of shrimp broodstock needs proper domestication and improvement of their genetic quality. MCBAD Jepara has conducted research to evaluate genetic heterogeneity of Penaeus monodon broodstock F1 using Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD) analysis. For RFLP analysis, amplification product of 16SrDNA of mitochondrial DNA was digested with restriction enzymes. According to the RFLP analysis, heterogeneity value of P. monodon F1 broodtock population was 0.0422; male F1 population was 0.0613 and female F1 population was 0.1252. The primer used in RAPD analysis was OPA 2. According to the RAPD analysis, heterogeneity value of P. monodon F1 broodstock population was 0.0417; male F1 population was 0.0653 and female F1 population was 0.1104. The results from these researches have shown that either RFLP or RAPD can be used as a family specific marker for Penaeus monodon.

Keywords


Genetic marker, Heterogeneity, Penaeus monodon, RFLP, RAPD

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DOI: https://doi.org/10.22146/jfs.2968

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