The 16S and COI Mitochondrial DNA Nucleotide Composition of Stripped Snakehead (Channa striata Bloch,1793) from Lake Sentani, Jayapura, Papua

https://doi.org/10.22146/jfs.35551

Christine Bawaeda Sitandung Kombong(1), Tuty Arisuryanti(2*)

(1) Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
(2) Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Stripped snakehead (Channa striata Bloch,1793) has potency to be developed in Indonesia due to high nutritional value including omega 3 which is useful for children growth. However, study on composition of mitochondrial DNA nucleotide as a part of study on genetic characterization of stripped snakehead fish in Indonesia is poorly
understood. Genetic characterization of stripped snakehead is important to obtain data information that can be applied for breeding and conservation program. Therefore, the objective of this study was to determine the 16S and COI mitochondrial DNA nucleotide of stripped snakehead collected from Lake Sentani, Jayapura, Papua. Method used in this research is PCR method using primer 16Sar and 16Sbr for 16S mitochondrial gene, and FishF2 and FishR2 for COI mitochondrial gene. The results showed that composition of 16S mtDNA nucleotide from 616 bp was T=21.92%, C=25.49%, A=30.19%, dan G=22.40%, whereas composition of A+T=52.11% and G+C=47.89%. In addition, composition of COI mtDNA nucleotide from 705 bp was T=29.93%, C=28.65%, A=24.26%, and G=17.16% while composition of A+T=54.48% and G+C=45.52%. Data of 16S and COI mitochondrial DNA nucleotide composition of the stripped snakehead collected from Lake Sentani gained in this study are to be expected to complete genetic information of the fish species which is useful to improve breeding program of this fish species in the future.

Keywords


16S mtDNA; COI mtDNA; stripped snakehead; genetic charcterization; nucleotide composition

Full Text:

PDF


References

Abol‐Munafi, A.B., B.M Tarn., M.A. Ambak & P. Ismail. 2004. Effect of different diets on growth and survival rates of snakehead (channa striatabloch, 1797) larvae. Korean. J. of Biological Sciences, 8 (4) : 313-317. doi: 10.1080/12265071.2004.9647766

Amilhat, E & K. Lorenzen, 2005. Habitat use, migration pattern and population dynamics of chevron snakehead Channa striata in a rainfed rice farming landscape. J. of Fish Biology. 67: 23-24. doi: 10.1111/j.1095-8649.2005.00927.x

Aquino, L.M., J.M. Tango., R.J. Cano., I.K. Fontanilla., Z.U. Basia., P.S. Ong & J.P. Quilang. 2011. DNA barcoding of fishes of Laguna de Bay, Phillipines. Mitochondrial DNA, 22 (4): 143-153. 10.3109/19401736.2011.624613

Arif, I.A., H.A. Khan., A.H. Bahkali., A.A. Al Homaidan., A.H. Al Farhan., M. Al Sadoon, & M. Shobrak. 2011. DNA marker technology for wildlife conservation. Saudi J. of Biological Science, 18(3): 219-225. doi: 10.1016/j.sjbs.2011.03.002

Arisuryanti, T. 2016. Molecular genetic and taxonomic studies of the swamp eel (Monopterus albus Zuiew, 1793). Ph.D. Thesis. Charles Darwin University, Australia.

Arisuryanti, T., N-W.V. Wei & C. Austin. 2016. Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE). AIP Conferences Proceedings, 1744(020060). doi: 10.1063/1.4953534

Benziger, A., S. Philip., R. Raghavan., P.H. Anvar Ali., M. Sukumaran., J.C. Tharian, & A. Antunes. 2011. Unraveling a 146 years old taxonomic puzzle: validation of Malabar snakehead, species-status and its relevance for channid systematics and evolution. Public Library of Science One, 6 (6): e21272. doi: 10.1371/journal.pone.0021272

Bhat, A.A., M.A. Haniffa., M.J. Milton.,B.A. Paray., P.R. Divya & A. Gopalakrishnan. 2014. Genetic variation of striped snakehead (Channa striatus Bloch, 1793) populations using random amplified polymorphic DNA (RAPD) markers. International Journal of Biodiversity and Conservation, 6 (5): 363-372. doi: 10.5897/ijbc2013.0649

Chauhan, T & K. Rajiv. 2010. Molecular markers and their applications in fisheries and aquaculture. Advances in Bioscience and Biotechnology, 1(4): 281-291. doi: 10.4236/abb.2010.14037

Courtenay, W.R & J.D. Williams. 2004. Snakeheads (Pisces, Channidae)- A Biological Synopsis and Risk Assessment. U.S Geological Survey Circular 1251. Florida. page 6

Dahruddin, H., A. Hutama., F. Busson., S. Sauri., R. Hanner., P. Keith., R. Hadiaty & N. Hubert. 2017. Revisiting the ichthyodiversity of Java and Bali through DNA barcodes: taxonomic coverage, identification accuracy, cryptic diversity and identification of exotic species. Molecular Ecology Resources, 17 (2): 288-299. doi: 10.1111/1755-0998.12528

Dhar, B & S.K. GhoshK. 2015. Genetic assessment of ornamental fish species from North East India. Gene, 555( 2): 382-392. doi: 10.1016/j.gene.2014.11.037

Eschmeyer, W.N., R. Fricke & R. Van Der Laan (eds). 2018. Catalog Of Fishes: Classification. http://www.calacademy.org/scientists/catalog-of-fishes-classification/ diakses tanggal 24 Januari 2018

Ferdausi, H.J., N.C. Roy., M.J. Ferdous., M.A. Hossain., M.M. Hasan., B.D. Trina & M.M. Hossain. 2015. Reproductive biology of striped snakehead (Channa striata) from Wetlands of Sylhet, Bangladesh. Annals of Veterinary and Animal Science 2 (6): 162-169.

Hubert, N., V. Calcagn., R.S. Etienne & N. Mouquet, N. 2015. Metacommunity speciation models and their implications for diversification theory. [Research Support, Non-U.S. Ecology Letter, 18 (8): 864-881. doi: 10.1111/ele.12458

Kakkaeo, M., T. Chittapalapong & M.C. Villanueva. 2004. Food habits, daily ration and relative food consumption in some fish populations in Ubolratana reservoir, Thailand. Asian Fisheries Science 17: 249-259.

Kottelat, M. 2013. The Fishes of the Inland Waters of Southeast Asia: A Catalogue and Core Bibliography of the Fishes Known to Occur in Freshwaters, Mangroves and Estuaries Suplement 27. The Raffles Bulletin of Zoology. page 446 & 458.

Lakra, W.S., M. Goswami., A, Gopalakrishnan., D.P. Singh., A. Singh & N.S. Nagpure. 2010. Genetic relatedness among fish species of Genus Channa using mitochondrial DNA genes. Biochemical Systematics and Ecology, 38 (6): 1212-1219. doi: 10.1016/j.bse.2010.12.012

Laksmana, Y. 2010. Jelajah Jayapura: Eksotisme Alam Budaya di Pintu Gerbang Papua. PT.Gramedia Pustaka Utama. Jakarta. halaman 63-65

Librado, P & J. Rozas. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25 (11), 1451-1452. doi: 10.1093/bioinformatics/btp187

Lohman, D.J., M. de Bruyn., T. Page., K. Von Rintelen., R. Hall., P.K.L. Ng., T. Von Rintelen. 2011. Biogeography of the Indo-Australian Archipelago. Annual Review of Ecology, Evolution, and Systematics, 42 (1): 205-226. doi: 10.1146/annurev-ecolsys-102710-145001

Muchlisin, Z.A., A.S. Batubara., N. Fadli., A.A. Muhammadar., I.A. Utami., N. Farhana & M.N. Siti-Azizah. 2017. Assessing the species composition of tropical eels (Anguillidae) in Aceh Waters, Indonesia, with DNA barcoding gene cox1. F1000Research, 6: 258. doi: 10.12688/f1000research.10715.1

Ngili, Y., D. Lantang., R. Ubyaan., E. Imelda & I.Y. Palit. 2015. Mitochondrial DNA genetic diversity of arowana fish local variants on the D-loop hypervariable region: Study in the southern region of Papua-Indonesia. Der Pharma Chemica, 7 (10): 223-228.

Phen, C., T.B. Thang., E. Baran & L.S. Vann. 2005. WorldFish Center and Inland Fisheries Research and Development Institute. Cambodia. page 2-6

Purnamawati, D., K. Nirmala., E. Harris & R. Affandi. 2017. Survival and growth of striped snakehead fish (Channa striata Bloch.) juvenile reared in acid sulfate water and rainwater medium. Aquaculture, Aquarium, Conservation & Legislation-International Journal of Bioflux Society, 10 (2): 265-273.

Serrao, N.R., D. Steinke & R.H. Hanner. 2014. Calibrating snakehead diversity with DNA Barcodes: Expanding Taxonomic coverage to enable identification of potential and established invasive species. Public Library of Science one, 9 (6): e99546. doi: 10.1371/journal.pone.0099546.t001

Sood, N., D.K. Chaudhary., P.K. Pradhan., D.K. Verma., T. Raja Swaminathan., B. Kushwaha & J.K. Jena. 2015. Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793). In Vitro Cellular & Developmental Biology- Animal, 51 (8): 787-796. doi: 10.1007/s11626-015-9891-1

Tran, D.D., K. Shibukawa., T.P. Nguyen., P.H. Ha., X.L. Tran., V.H. Mai & K Utsugi. 2013. Fishes of the Mekong Delta, Vietnam. Can Tho University Publishing House. Can Tho City. page125

Ward, R.D., T.S. Zemlak., B.H. Innes., P.R. Last & P.D. Hebert. 2005. DNA barcoding Australia's fish species.Philosophical Transactions of the Royal Society of London series B Biological Sciences, 360 (1462), 1847-1857. doi: 10.1098/rstb.2005.1716



DOI: https://doi.org/10.22146/jfs.35551

Article Metrics

Abstract views : 2871 | views : 6162

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2018 Jurnal Perikanan Universitas Gadjah Mada

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

View My Stats