* Corresponding Author
Isolation of Cellulolytic Bacterium Staphylococcus sp. JC20 from the Intestine of Octopus (Octopus sp.) for Fish Probiotic Candidate
https://doi.org/10.22146/jfs.39525 Indah Istiqomah(1*), Alim Isnansetyo(2), Imelda Novita Atitus(3), Ahmad Fauzi Rohman(4)
(1) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta
(2) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta
(3) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta
(4) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author
Abstract
Aim of this study was to isolate, characterize, and identify cellulolytic bacteria from the digestive tract of marine vertebrates and invertebrates as a candidate of fish probiotics. The bacteria were isolated from the digestive tract and grown on a cellulose agar plate. The bacteria were screened based on the cellulolytic activity, acid resistance, antagonist activity against fish pathogens, antibiotics sensitivity, ability to live in fish digestive tract and non-pathogenic test. Selected bacterium was identified molecularly, based on the 16S rDNA gene sequences, and phenotipically. A total of 14 bacteria demonstrated celulolitic index of 1.1-1.8. The bacteria with cellulolytic index of > 1.6 were screened by the selection criteria, resulted a selected strain, JC20 isolate which was isolated from the digestive tract of octopus (Octopus sp.). The selected bacterium was sensitive to antibiotics, resists to acidic environment, able to live in the fish digestive tract, and non-pathogen. Thus, the bacterium was potential for further characterization as fish probiotics candidate. Molecular and phenotypic identification revealed that JC20 isolate was Staphylococcus sp.
Keywords
Full Text:
PDFReferences
Altschul, S., G. Warren, W. Miller, E. Myers & D. Lipman. 1990. Basic local alignment search tool. Journal of Molecular Biology. 215 (3): 403-410. DOI:10.1016/S0022-2836(05)80360-2
Balcazar, J.L., I. de Blas, I. Ruiz-Zarzuela, D. Cunningham, D. Vendrell & J.L Muzquiz. 2006. The role of probiotics in aquaculture. Vet. Microbiol. 114: 173-186
Castillo, S & D.M. Gatlin. 2015. Dietary supplementation of exogenous carbohydrase enzymes in fish nutrition: A review. Aquaculture 435: 286-292
Davis, C. 2014. Enumeration of probiotic strains: Review of culture-dependent and alternative techniques to quantify viable bacteria. Journal of Microbiological Methods. 103: 9-17
Dioundick, O.B & D.I. Stom. 1990. Effects of detary a-cellulose levels on the juvenile tilapia, Oreochromis mossambicus (Peters). Aquaculture 91: 311-315
Erdiandini, I.T.C., Sunarti & A. Meryandini. 2015. Seleksi bakteri asam laktat dan pemanfaatannya sebagai starter kering menggunakan matriks tapioka asam. Jurnal Sumberdaya Hayati. 1 (1): 26-33
Gatesoupe, F.J. 1999. The use of probiotics in aquaculture. Aquaculture. 180: 147-165
Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley & S.T. Williams. 1994. Bergey’s Manual of Determinative Bacteriology Ninth Edition. Williams & Wilkins. United Stated of America
Huang, S., P. Sheng & H. Zhang. 2012. Isolation and identificarion of cellulolytic bacteria from the gut of Holotrichia parallela larvae (Coleoptera: Scarabaeidae). International Journals of Molecular Sciences. 13: 2563-2577
Irianto, A., H. Hernayanti & N. Iriyanti. 2006. Pengaruh suplementasi probiotik a3-51 terhadap derajat imunitas Oreochromis niloticus didasarkan pada angka kuman pada ginjal setelah uji tantang dengan Aeromonas hydrophila dan Aeromonas salmonicida achromogenes. Jurnal Perikanan Universitas Gadjah Mada. 8 (2): 144-152
Isnansetyo, I., I. Istiqomah, Triyanto & J. Widada. 2009. A potential bacterial biocontrol agent, strain S2V2 against pathogenic marine Vibrio in aquaculture. 1103-1113. https://doi.org/10.1007/s11274-009-9992-7
Kurniasih, T., Widanarni, Mulyasari, I. Melati, Z.I. Azwar & A.M. Lusiastuti. 2013. Isolasi, seleksi, dan identifikasi bakteri dari saluran pencernaan ikan lele sebagai kandidat probiotik. Jurnal Riset Akuakultur. 8 (2): 227-286
Lavanya, K & Y. Dayakar. 2017. Isolation and characterization of probiotic bacteria from the soil samples of the coastal areas of (Gudur division, Nellore Dt.) for utilization in Shrimp farming. International Journal of Fisheries and Aquatic Studies. 5 (4): 371-376
Mulyasari., Widanarni, M.A. Suprayudi, M.Z. Junior & M.T.J. Sunarno. 2015. Seleksi dan identifikasi bakteri selulolitik pendegradasi daun singkong (Manihot esculenta) yang diisolasi dari saluran pencernaan ikan gurame (Osphronemus gouramy). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 10 (2): 111-121
Schlegel G.H & S. Karin. 1994. Mikrobiologi Umum, Edisi Keenam. Diterjemahkan oleh Tedjo Baskoro dan Joke R. Wattimena. UGM Press. Yogyakarta
Soeprijanto, A., G. Guntur dan M. Fakhri. 2018. Application of probiotic and fermented feed in the nursery of Anguilla bicolor. Jurnal Perikanan Universitas Gadjah Mada. 20 (1): 19-22. https://doi.org/10.22146/jfs.34207
Tommey N., D. Bolton, & S. Fanning. 2010. Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs. Journal Research in Microbiology. 161: 127-135.
Verchuere, L., G. Rombaut, P. Sorgeloos & W. Verstraete. 2000. Probiotics bacteria as biological control agents in aquaculture. Microbiol. Mol. Biol. Rev. 64: 655–671
Wilson, R.P. 1994. Utilization of dietary carbohydrate by fish. Aquaculture. 124: 67-80
DOI: https://doi.org/10.22146/jfs.39525
Article Metrics
Abstract views : 6737
| 
views : 20856
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Jurnal Perikanan Universitas Gadjah Mada
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
View My Stats