Seahorse Hydrolisate (Hippocampus kuda) and Anti-Inflammatory Activity Test with Protein Denaturation Inhibition Method
Nur Azizah Nasution(1), Mala Nurilmala(2*), Asadatun Abdullah(3)
(1) Faculty of Fisheries and Marine Science, Institut Pertanian Bogor. Bogor
(2) Faculty of Fisheries and Marine Science, Institut Pertanian Bogor. Bogor
(3) Faculty of Fisheries and Marine Science, Institut Pertanian Bogor. Bogor
(*) Corresponding Author
Abstract
Seahorse is marine biota that have commercial value and used as ornamental fish and traditional medicinal ingredients. Utilization of seahorses has still not been extensively studied, especially in the form of hydrolysates. Hydrolysates of seahorses are known to have efficacy in biological activity. This study was conducted to identify seahorse morphometrics, determine selected enzymes based on the degree of hydrolysis and determine the anti-inflammatory activity of hydrolysates. The results showed that morphometric identification showed morphological characteristics of Hippocampus kuda, namely the number of body rings 12 units, tail ring 35 units, eye protrusion and cheeks as much as 1 unit, average head length of 3.5 cm, number of body rings on dorsal fin 3 units. The best degree of hydrolysis of seahorses is protein hydrolysis using alkalase enzyme of 42.49%. The highest anti-inflammatory activity on sea horse hydrolyzate has %inhibition of 42.88%.
Keywords
Full Text:
PDFReferences
Adam, K., L. Fitria & M. Sarto. 2014. Pengaruh pemberian fraksi protein ekstrak kuda laut (Hippocampus kuda Bleeker, 1852) terhadap peningkatan kadar hemoglobin mencit (Mus musculus L). Jurnal Kefarmasian Indonesia. 4 (2) :83-90
Arafah, P. 2017. Karakteristik gelatin kulit ikan tuna (Thunnus albacares) dan potensinya sebagai antioksidan. Skripsi. Institut Pertanian Bogor. Bogor
Baehaki, A., S.D. Lestari & A.R. Romadhoni. 2015. Hidrolisis protein ikan patin menggunakan enzim papain dan aktivitas antioksidan hidrolisatnya. Jurnal Pengolahan Hasil Perairan.18 (30): 230-239
Charoenphun, N., B. Cheirsilp, N. Sirinupong & W. Youravong. 2013. Calcium-binding peptides derived from tilapia (Oreochromis niloticus) protein hydrolysate. European Food Research and Technology. 236: 57-63
Coultate, T.P. 2002. Food: The Chemistry of Its Components. Fourth Edition. Cambridge (UK): RSC Paperbacks
Erlina, R.A., Indah & Yanwirasti. 2007. Efek antiinflamasi ekstrak etanol kunyit (Curcuma domestica Val) pada tikus putih jantan galur wistar. Jurnal Sains dan Teknologi Farmasi. 12 (2): 112-115
FAO. 2010. Cultured Aquatic Species Information Programme : Hippocampus comes. FAO Fisheries and Aquaculture Department
Fawole, O.O., M.A. Ogundiran, T.A. Ayandiran & O.F. Olagunju. 2007. Proximate and mineral composition in some selected fresh water fishes in Nigeria. Internet Journal of Food Safety. 9: 52-55
Fawole, O.O., T.A. Yekeen, S.O. Adewoye, M.A. Ogundiran, O.E. Ajayi & M.N. Nwaiya. 2013. Nutritional qualities and trace metals concentration of six fish species from Oba reservoir Ogbomoso, Nigeria. African Journal of Food Science. 7 (8): 246- 252
Foster, S., S.Wiswedel & A. Vincent. 2016. Opportunities and challenges for analysis of wildlife trade using CITES data-seahorses as a case study. Aquatic Conservation: Marine and Freshwater Ecosystems. 26 (1): 154-172
Gauthier, S.F., C. Vachon, J.D. Jones & L. Savoie. 1982. Assessment of protein digestability in vitro enzymatic hydrolysis with simultaneous dialysis. Journal of Nutrition. 112: 1718-1725
Guo, Z., D. Lin, J. Guo, Y. Zhang & B. Zheng. 2017. In vitro antioxidant activity and in vivo anti-fatigue effect of seahorse (Hippocampus) peptides. Molecules. doi:10.3390/molecules22030482
Haslaniza, H., M.Y. Maskat, A.W.M. Wan & S. Mamot. 2010. The effects of enzyme concentration, temperature and incubation time on nitrogen content and degree of hydrolysis of protein precipitate from cockle (Anadara granosa) meat wash water. International Food Research Journal. 17: 147-152
Kang, N., S.Y. Kim, S. Rho, J.Y. Ko & Y.J. Jeon. 2017. Anti-fatigue activity of a mixture of seahorse (Hippocampus abdominalis) hydrolysate and red ginseng. Fisheries and Aquatic Sciences. 20: 3
Kumaravel, K., S. Ravichandran, T. Balasubramanian & K.S. Subramanian. 2010. Antimicrobial effect of five seahorse species from indian coast. British Journal of Pharmacology and Toxicology. 1: 62-6
Lin, Q., L. Junda, L. Junyi & L. Bingsi. 2008. Biochemical composition of six seahorse species Hippocampus sp from the chinese coast. World Aquaculture Society. 39 (2): 225-234
Lourie, S.A., S.J. Foster, E.W.T. Cooper & A.C.J. Vincent. 2004. A guide to the
identification of seahorses. Project seahorse and traffic. North America (US): University of British Columbia and World Wildlife Fund
Mufidah, S. 2014. Modifikasi struktur senyawa etil p-metoksisinamat yang diperoleh dari kencur (Kaempferia galanga Linn.) melalui transformasi gugus fungsi serta uji aktivitas sebagai antiinflamasi. Skripsi. Fakultas Kedokteran dan Ilmu Kesehatan UIN Syarif Hidayatullah. Jakarta
Oyedapo, O.O., B.A. Akinpelu, K.F. Akinwunmi, M.O. Adeyinka & F.O. Sipeolu. 2010. Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. International Journal of Plant Physiology and Biochemistry. 2 (4): 46-51
Pangestuti, R., B. Ryu, S.W.A. Himaya & S.K. Kim. 2013. Optimization of hydrolysis conditions, isolation, and identification of neuroprotective peptides derived from seahorse Hippocampus trimaculatus. Amino Acids. 45: 369-381
Rabiansyah. 2015. Studi Ekologi Kuda Laut (Hippocampus) di Perairan Desa Sebong Pereh Kecamatan Teluk Sebong Kabupaten Bintan. Universitas Maritim Raja Ali. Kepualauan Riau
Rinayanti, A., E. Dewanti & M.H. Adelina. 2014. Uji efek antiinflamsi fraksi air daun mahkota dewa (Phaleria macrocarpa (Shecff.) Boerl.) terhadap tikus putih (Rattus norvegicus L.). Pharmaceutical Sciences and Research. 1 (2): 78-85
Ryua, B., Z.J. Qianb & S.K. Kim. 2010. Purification of a peptide from seahorse, that inhibits TPA-induced MMP, iNOS and COX-2 expression through MAPK and NF-_B activation, and induces human osteoblastic and chondrocytic differentiation. Chemico-Biological Interactions. 413-422
Sanaye, S.V., N.M. Pise, A.P. Pawar, P.P. Parab, R.A. Sreepada, H.B. Pawar & A.D. Revankar. 2014. Evaluation of antioxidant activities in captive-bred cultured yellow seahorse Hippocampus kuda (Bleeker, 1852). Aquaculture. 1-34. doi 10.1016/j.aquaculture.2014.08.007
Santoso, B. 2014. Analisis jenis makanan kuda laut Hippocampus barbouri, (Jordan & Richardson, 1908) pada daerah padang lamun di kepulauan tanakeke, takalar, sulawesi selatan. Skripsi. Fakultas ilmu kelautan dan perikanan. Universitas Hasanuddin. Makassar
Sari, E.M. 2018. Identifikasi kuda laut berdasarkan marka molekuler serta potensinya sebagai antioksidan, antiglikasi dan imunomodulator. Tesis. Bogor. Institut Pertanian Bogor
Sari, E.M., M. Nurilmala & A. Abdullah. 2017. Profil asam amino dan senyawa bioaktif kuda laut Hippocampus comes. Jurnal Ilmu dan Teknologi Kelautan Tropis. 9 (2): 605-617
Uju, T., Nurhayati, B. Ibrahim, W. Trilaksani & M. Siburian. 2009. Karakterisasi dan recovery protein dari air cucian minced fish dengan membran reverse osmosis. Jurnal Pengolahan Hasil Perikanan Indonesia. 12 (2) :115-127
Verma M.A, A.P. Kumar, D. Kavitha & K.B. Anurag. 2011. Anti denaturation and antioxidant activities of Annona cherimola in vitro. International Journal of Pharma and Bio Sciences. 2 (2) :1-6
Widyakarya Nasional Pangan dan Gizi [WNPG]. 2004. Pangan dan Gizi. Jakarta. Lembaga Ilmu Pengetahuan Indonesia.
Xu, D., X. Mei, B. Lin & S. Xu. 2003. The pharmacological effects of Hippocampus capsule on enhancing sexual functions of rats. Chinese Medicine. 26 (11): 807-808
Zhang, H., Y. Luo & S.D. Luo. 2001. Affect of the sea horse Hippocampus japonicus on pituitary gonadal axis in male rats. Chinese Journal of Marine Drugs. 2: 39-41
DOI: https://doi.org/10.22146/jfs.43699
Article Metrics
Abstract views : 6656 | views : 13266Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Jurnal Perikanan Universitas Gadjah Mada
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
View My Stats