* Corresponding Author
Partial Characterization of Chitosanase from Bacillus cereus Strain BFE5400 Isolated from Snakehead Fish Intestine
https://doi.org/10.22146/jfs.43746 Shanti Dwita Lestari(1*), Ace Baehaki(2), Guttifera Guttifera(3)
(1) Program Studi Teknologi Hasil Perikanan, Fakultas Pertanian, Universitas Sriwijaya, Palembang, Sumatera Selatan, Indonesia
(2) Program Studi Teknologi Hasil Perikanan, Fakultas Pertanian, Universitas Sriwijaya, Palembang, Sumatera Selatan, Indonesia
(3) PDD Akademi Komunitas Negeri Banyuasin, Palembang, Sumatera Selatan, Indonesia
(*) Corresponding Author
Abstract
Feeding pattern of wild snakehead fish (Channa striata) which includes shrimp and small crustaceans makes it is intestine a good source of chitinase and chitosanase enzymes. This study aimed to isolate chitosanase producing bacteria from snakehead fish intestine, to select isolate with highest chitosanolytic activity, to produce chitosanase using the selected isolate and partially characterize the resulting enzyme. I2 isolate exhibited the highest chitosanolytic index with the value of 1.5 and produced enzyme with an activity of 0.00331 U/mL after 72 hours incubation. The ultimate bacterial density was at the 24th hour with a turbidity value of 0.5315. The enzyme activity was optimum at pH 6, with a value of 0.00816 U/mL, while the optimum temperature was 70°C with the enzyme activity of 0.00785 U/mL. Genetic identification of I2 isolate using 16S rRNA showed that the isolate has a high similarity to Bacillus cereus strain BFE 5400.
Keywords
Full Text:
PDFReferences
Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith & K Struhl. 1997. Current Protocols in Molecular Biology. John Wiley & Sons, Inc., Hoboken
Chasanah, E., Y.N. Fawzya, A. Pratitis & T. Nurhayati. 2007. Penapisan bakteri penghasil enzim kitosanase yang berasosiasi dengan spons laut. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 2( 2): 161-169
Chasanah, E., G. Patantis, D.S. Zilda, M. Ali & Y. Risjani. 2011. Purification and characterization of Aeromonas media KLU 11.16 chitosanase isolated from shrimp waste. Journal of Coastal Development. 15 (1): 104-113
Chiang, C.L., C.T. Chang & H.Y. Sung. 2002. Purification and properties of chitosanase from a mutant of Bacillus subtilis IMR-NK1. Enzyme and Microbial Technology. (32): 260-267
Choi Y.J., E.J. Kim, Z. Piao, Y.C. Yun & Y.C. Shin. 2004. Purification and characterization of chitosanase from Bacillus sp. strain KCTC 0377BP and its application for the production of chitosan oligosaccharides. Applied and Environmental Microbiology. 70: 4522-4531
Ferrari, A.R., Y. Gaber & M.W. Fraaije. 2014. A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection. Biotechnology for Biofuels. 7: 37
Gao, X.A., W.T. Ju, W.J. Jung & R.D. Park. 2008. Purification and characterization of chitosanase from Bacillus cereus D-11. Carbohydrate Polymers. (72): 513-520
Haliza W. 2003. Karakteristik Kitosanase Unik dari Bacillus coagulans LH 28.38 Asal Lahendong Sulawesi Utara. Tesis S2. Pascasarjana IPB, Bogor. https://repository.ipb.ac.id/handle/123456789/7993
Ibrahim, K.A., B.I. El-Eswed, K.A. Abu-Sbeih, T.A. Arafat, M.M.H. Al Omari, F.H. Darras & A.A. Badwan. 2016. Preparation of chito-oligomers by hydrolysis of chitosan in the presence of zeolite as adsorbent. Marine Drugs. 14 (43)
Kasaai, MR., J. Arul & G. Charlet. 2008. Fragmentation of chitosan by ultrasonic irradiation. Ultrason Sonochem. 15 (6): 1001-1008
Kasaai, M.R., J. Arul & G. Charlet. 2013. Fragmentation of Chitosan by Acids. The Scientific World Journal
Kurakake, M., S. Yo-u, K. Nakagawa, M. Sugihara & T. Komaki. 2000. Properties of chitosanase from Bacillus cereus S1. Current Microbiology. 40 (1): 6-9
Mathiavanan, N., V. Kabilan & K. Murugesan. 1998. Purification, characterization and antifungal activity of chitinase from Fusarium chlamydosporum, a microparasite to groundnut rust, Puccina arachidis. Canadian Journal of Microbiology. 44:646-651
Muchtadi, D., N.S. Palupi & M. Astawan. 1996. Enzim dalam Industri Pangan. PAU Pangan dan Gizi Institut Pertanian Bogor, Bogor, Indonesia.
Oh, C., M. De Zoysa, D-H. Kang, Y. Lee, I. Whang, C. Nikapitiya, S-J. Heo, K-T. Yoon, A. Affan & J. Lee. 2011. Isolation, purification, and enzymatic characterization of extracellular chitosanase from marine bacterium Bacillus subtilis CH2. Journal Microbiology Biotechnology. 21 (10): 1021-1025
Pagnoncelli, M.G.B., N.K. de Araújo, N. M.P. da Silva N.M.P, C.F. de Assis, S. Rodrigues & G.R. de Macedo. 2010. Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis. Brazilian Archives of Biology and Technology. 53 (6): 1461-1468
Rydz, J., M. Musioł, B. Zawidlak-Węgrzyńska & W. Sikorska. 2018. Present and future of biodegradable polymers for food packaging applications. Biopolymers for Food Design. 431-467
Setia, I.N & Suharjono. 2015. Chitosanolytic assay and identification of bacteria isolated from shrimp waste based on 16S rDNA sequences. Advances in Microbiology. 5: 541-548
Shahidi, F., J.K.V. Arachchi & Y.J. Joen. 1999. Food application of chitin and chitosan. Trends in Food Science and Technology. (10): 37-51
Somashekar, D & R. Joseph. 1996. Chitosanases - properties and applications: a review. Bioresource Technology. 55 (1): 35-45
Suhartono, M.T. 1989. Enzim dan Bioteknologi. Institut Pertanian Bogor, Bogor, Indonesia.
Sun, T., D. Zhou, J. Xie & F. Mao. 2007. Preparation of chitosan oligomers and their antioxidant activity. European Food Research and Technology. 225 (3-4): 451-456
Swiontek-Brzezinska, M., E. Lalke-Porczyk & W. Donderski. 2007. Chitosanolytic activity of bacteria and fungi isolated from shrimp exoskeletons.Oceanological and Hydrobiological Studies. 36 (3): 101-111
DOI: https://doi.org/10.22146/jfs.43746
Article Metrics
Abstract views : 1858
| 
views : 1642
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Jurnal Perikanan Universitas Gadjah Mada
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
View My Stats