HUBUNGAN PERKEMBANGAN MORFOLOGI DENGAN ORGAN PENCERNAAN LARVA KERAPU BEBEK (Cromileptes altivelis) TURUNAN KE-3 (F-3)

https://doi.org/10.22146/jfs.9052

Wawan Andriyanto(1*), Muhammad Marzuqi(2)

(1) Universitas Gadjah Mada
(2) Balai Besar Penelitian dan Pengembangan Budidaya Laut Balitbang Kelautandan Perikanan, Kementerian Kelautan, dan Perikanan, Gondol
(*) Corresponding Author

Abstract


Pengamatan perkembangan morfologi dan perkembangan saluran pencernaan larva ikan kerapu bebek F3 hasil pembenihan induk generasi F2 telah dilakukan melalui serangkaian pemeliharaan dari umur 1 HSM (hari setelah menetas) sampai dengan 40 HSM. Sampel larva diambil setiap hari dari 1 HSM sampai dengan 10 HSM dan dilanjutkan interval 3 hari sampai umur 40 HSM. Pakan alami diberikan sesuai dengan SOP perbenihan kerapu. Sampel dianalisa secara histologi dan morfologi dengan piranti lunak yang mendukung. Fase yolk sac sampai dengan pre fl exionsecara pertumbuhan tidak signifi kan, namun perkembangan morfologi dan differensiasi saluran pencernaan berkembang sangat pesat. Pada fase ini organ pencernaan larva sudah terbagi dari mulut, esophagus, perut, usus dananus namun belum sempurna. Pada fase akhir pre fl exion (13 HSM) pertumbuhan larva menunjukkan grafi k eksponensial (tumbuh cepat). Notochord terbentuk pada fase fl exion (18-20 HSM), sementara organ pencernaan terutama perut dan usus mulai berkembang. Perkembangan morfologi stadia larva kerapu bebek turunan ke-3 (F3) dari fase yolk sac sampai dengan juvenil memerlukan waktu sekitar 28 sampai dengan 31 HSM. Proporsi duri sirip punggung pada stadia prefl exion sampai dengan postfl exion memiliki panjang yang cenderung lebih tinggi dibanding kerapu yang lain yaitu 76%. Rata rata pertumbuhan panjang larva kerapu dari stadia yolk sac sampai dengan juvenil dicapai sebesar 0.41% dengan total panjang larva pada stadia juvenil adalah sekitar 18.42 ± 3.23 mmTL. Nilai standar deviasi yang tinggi memperlihatkan variasi ukuran pada akhir stadia. Larva kerapu bebek turunan ke-3 mulai memanfaatkan makanan dari luar sekitar umur 4 HSM dan saluran pencernaan mulai berfungsi optimal pada umur 28–31 HSM.


Keywords


f3; kerapu bebek; larva; morfologi; organ pencernaan



References

Munafi , A.B., W. Andriyanto, S. Ismi, A.Y. Nirmala, I. Mastuti, A. Muzaki & A.W.M. Effendy. 2011. The Ontogeny of the Digestive Tract and Associated Organs of Humpback Grouper (Cromileptes altivelis) Larvae. J. Asian Fish Sci. 24:379-386.

Ariza, S.A. 2010. Ontogenetic Digestive System and Enzyme Activity in Early Life of Tiger Grouper Epinephelus fuscoguttatus, Thesis of Master of Science. Universiti Malaysia Terengganu. Unpublished.

Baglole, C.J., H.M. Murray, G.P. Goff & G.M. Wright. 1997. Ontogeny of the digestive tract during larval development of yellowtail flounder: a light microscopic and mucous histochemical study. J. Fish Biol. 51:120–134.

Bisbal, G.A. & D.A. Bengtson.1995. Development of the digestive tract in larval summer fl ounder. J. Fish Biol. 47: 277–291.

Cahu, C.& J.Z. Infante. 2001. Substitution of live food by formulated diets in marine fish larvae. J. Aquac. 200:161–180.

Canino, M.F. & K.M. Bailey. 1995. Gut evacuation of walleye Pollock larvae in response to feeding conditions. J. Fish Biol. 46:389–403.

Chen, B.N., J.G. Qin, M.S. Kumar, W. Hutchinson & S. Clarke. 2006. Ontogenetic development of the digestive system in yellowtail kingfi sh Seriola lalandi larvae. J. Aquac. 256: 489–501.

Deplano, M., J.P. Diaz, R. Connes, M. Kentouri-Divanach & F. Cavalier. 1991. Appearance of lipid-absorption capacities in larvae of the sea bass Dicentrarchus labrax during transition to the exotrophic phase. J. Mar. Biol. 108:361–371.

Drury, R.A. & E.A. Wallington. 1967. Carleton’s Histological Technique, 4th edition. Oxford: Oxford University Press.

Giri, N.A., K. Suwirya & M. Marzuqi. 1999. Kebutuhan protein, lemak, dan vitamin C untuk yuwana ikan kerapu bebek (Cromileptes altivelis). J. Pen Perik Ind. 5(3):38-46.

Giri, N.A., K. Suwirya & M. Marzuqi. 2006. Observation On Gonad Maturation Of The First Generation (F1) Of Humpback Grouper, Cromileptes altivelis. J. Indo. Aquac.: 1 (2):79-86.

Glamuzina, B., B. Skaramuca, N. Glavic, V. Kozul, J. Dulcic & M. Kraljevic. 1998. Egg and early larval development of laboratory reared dusky grouper, Epinephelus marginatus (Lowe, 1834) (Picies, Serranidae)*. J. Scientia Marina, 62 (4): 373-378.

Govoni, J.J., G.W. Boehlert & Y. Watanabe. 1986. The physiology of digestion in fish larvae. J. Env. Biol. Fish. 16: 59–77.

Hamlin, H.J., I. Hunt von Herbing & L.J. King. 2000. Histological and morphological evaluations of the digestive tract and associated organs of haddock throughout post hatching ontogeny J. of Fish Bio. 57:716–732.

Hopkins, K.D. 1992. Reporting fish growth: a review of the basics. J. World Aquac. Soc. 23:173–179.

Kawabe, K. & H. Kohno. 2009. Morphological development of larval and juvenile blacktip grouper, Epinephelus fasciatus. The Japan Soc of Fish Sci. 75:1239–1251.

Kato, K., K. Ishimaru, Y. Sawada, J. Mutsuro, S. Miyashita, O. Murata & H. Kumai. 2004. Ontogeny of digestive and immune system organs of larval and juvenile kelp grouper Epinephelus bruneus reared in the laboratory. J. Fish. Sci. 70:1061–1069.

Kiernan, J.K. 1990. Histological and Histochemical Methods theory and practice.Second edition. Pergamon Press plc. 433p.

Kjorsvik, E., K. Pittman & D. Pavlov. 2004. From fertilisation to the end of metamorphosis-functional development. In: Moksness, E., Kjorsvik, E., Olsen, Y. (Eds.), Culture of Cold-Water Marine Fish. Blackwell Publishing, Carlton, Victoria, 204–278pp.

Kohno, H., S. Diani & A. Supriatna. 1993. Morphological development of larval and juvenile grouper, Epinephelus fuscoguttatus. J. Japan Ichthyol. 40:307–316.

Martinez-Lagos, R. & V. Gracia-Lopez. 2009. Morphological development and growth patterns of the leopard grouper Mycteroperca rosacea during larval development. AquacResearch. 41: 120-128.

May, R.C. 1974. Larval mortality in marine fishes and the critical period concept. In: Blaxter, J.H.S. (Ed.), The Early Life History of Fish. Conference: International Symposium on the Early Life History of Fish. Springer, Berlin, 3–19 pp.

Mishima, M. & Gonzares. 1994. Some biological aspects on Cromileptes altivelis around Palawan Island, Philippines. Suisanzoshoku, 42(2): 354– 349 (in Japanese).

Pena, R., S. Dumas, M. Villalejo-Fuerte & J.L. Ortiz-Galindo. 2003. Ontogenetic development of the digestive tract in reared spotted sand bass Paralabrax maculatofasciatus larvae. J. Aquac. 219:633–644.

Quinitio, G.F., A.C. Sa-an, J.D. Toledo & J.D. Tan-Fermin. 2004. Advances in Grouper Acquaculture Edited by M.A. Rimmer, S. McBride and K.C. Williams ACIAR Monograph 110: 26-29.

Rimmer, M.A. 1998. Grouper and snapper aquaculture in Taiwan. Austasia Aquac.12(1): 3-7.

Rimmer, M.A. 2000. Review of grouper hatchery technology Grouper Aquaculture Electronic Newsletter, 27 January Queensland Department of Primary Industries, Northern Fisheries Centre, Cairns, Queensland, Australia.

Sarasquete, A. Polo & M. Yufera. 1995. Histology and histochemistry of the development of the digestive system of larval gilthead seabream, Sparus aurata L., J.Aquac.130: 79–92.

Segner, H., V. Storch, M. Reinecke, W. Kloas & W. Hanke. 1994. The development of functional digestive and metabolic organs in turbot, Scophthalmus maximus. Mar. Biol. 119:471–

Stroband, H.W.J. & K.R. Dabrowski. 1981. Morphological and physiological aspects of digestive system and feeding in resh-water fish larvae. In: Nutrition des Poissons (Fontain,M.,ed.), Centre National de la Rechereche Scientifi que. Paris. 355-374.



DOI: https://doi.org/10.22146/jfs.9052

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