ISOLASI, KARAKTERISASI, DAN PATOGENITAS BAKTERI PENYEBAB PENYAKIT PADA GURAMI (Osphronemus goramy) DI KABUPATEN BANTUL

https://doi.org/10.22146/jfs.9102

Murwantoko Murwantoko(1*), Rozi Rozi(2), Indah Istiqomah(3), Kamiso H. Nitimulyo(4)

(1) Jurusan Perikanan, Fakultas Pertanian UGM
(2) Jurusan Perikanan, Fakultas Pertanian UGM
(3) Jurusan Perikanan, Fakultas Pertanian UGM
(4) Jurusan Perikanan, Fakultas Pertanian UGM
(*) Corresponding Author

Abstract


Gouramy (Osphronemusgoramy) is an important fish comodity and preferred to be consumed by people. One of the limiting factor in freshwater aquaculture is bacterial infections. The objective of this research was to identify the species of pathogenic bacteria on gourami from Bantul Regency and to determine their pathogenicity. Eighteen diseased gouramy were collected from seven different fi sh farms. Bacteria were isolated from kidney and purifyon TSA medium. Characterization was conducted on the morphology of bacterial colonies and cells, biochemical tests. Koch Postulate Test was done to confirm pathogenic properties of the isolated bacteria. Pathogenicity test was performed by intraperitoneal injection of a serial doses of bacteria into gouramy and the Lethal Dosage 50 (LD50) was calculated. Twenty isolates of Aeromonas hydrophila, 5 isolates of Aeromonas salmonicida and one isolate of Vibrio cholerae,Vibrio anguillarum and Pseudomonas fluorescens respectively were isolated from and confi rmed as pathogen ingouramy. Pathogenic diversity was found among isolated bacteria, which a group of virulent bacteria as Aeromonas hydrophila GBNB, GGRB, and GCMA3 with LD50 values of 6.28 x 105, 2.09 x 105 and 5.37 x 105 CFU /fish; and a group of non virulent bacteria as A. hydrophila GKRA isolate and Vibrio cholera GPDA isolat with LD50 value of 7.14 x 107 and 3.45 x 107 CFU/fish respectively.


Keywords


characterization, gouramy (Osphronemus goramy), identication, Koch’s postulate, pathogenic bacteria

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References

Austin, B. & D.A. Austin. 2007. Bacterial fish Pathogens: diseases of farmed and wild Fish. 4 th edition. Praxis Publishing.

Cipriano, R.C., G.L. Bullock & S.W. Pyle. 1984. Aeromonas hydrophila and motile aeromonad septicemias of fi sh. U.S.Fish & Wildlife Publication Paper 134.

Citarasu,T., A.K. Dhas, S. Velmurugan, V.T. Thanga, T. Kumaran, M.M. Babu & T. Selvaraj. 2011. Isolation of Aeromonas hydrophila from infected ornamental fi sh hatchery during massive disease outbreak. Int. J. Curr. Res., 2:37-41.

Dana, D. & S.L. Angka. 1990. Masalah penyakit parasit dan bakteri pada ikan air tawar serta cara penanggulangannya. Proseding Seminar Nasional II Penyakit Ikan dan Udang. Bogor, 16-19 Januari 1990

Hardaningsih, I., Murwantoko & S. Helmiati. 2012. Tujuh rezeki budidaya gurami. Kanisius. Yogyakarta. 95 p.

Holt, J.G., N.R. Kreig, P.H.A. Sneath, J.T. Staley, & S.T. Williams.1994. Bergey’s manual of determinative microbiology. 9thed. The Williams & Wilkins Co, Baltimore.

Hsu, T.C., E. B. Shotts, & W.D. Waltman. 1985. Action of Aeromonas hydrophila complexon carbohydrate substrates. Fish Pathology. 20: 23 - 35.

Hubert, J.J. 1980. Bioassay. Kendall/Hunt Publishing Company, Lowa, USA.

Jutono, J., S. Soedarsono, S. Hartadi, S. Kabirun, Suhadi, & D. Soesanto. 1973. Pedoman Praktikum Mikrobiologi (umum untuk Perguruan Tinggi). Departemen Mikrobiologi Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta

Kamiso, H.N. 1986. Fish Disease as a problem in intensive aquaculture. Proceeding seminar multy disciplinary studies on fisheries and inshore coastal Soedarsono (Ed.) Semarang, 21-26 juli 1986: 416-438.

Kamiso, H.N., A. Sarono, I.Y.B. Lelana, N. Widodo, E. B. Thaib, S. Haryani, Haryanto, Triyanto, Ustadi, A. N. Kusumahati, W. Novianti, S. Wardani, & Setianingsih. 1993. Hama dan penyakit ikan golongan bakteri: buku 2. Pusat Karantina Pertanian dan Jurusan Perikanan. Fakultas Pertanian. Universitas Gadjah Mada. Yogyakarta.

Kamiso, H. N.,1996. Vibriosis pada ikan dan alternatif penanggulangannya. Jurnal Perikanan I (1): 7886. Khairuman & K. Amri. 2003. Pembenihan dan pembesaran gurami secara intensif. PT Agromedia Pustaka, Jakarta.

Macfaddin, J.F. 1980. Biochemical test for identifi cation of medical bacteria. 2nd ed. Williams & Wilkins,

Waverly Press, Inc. Mt. Royal, and Guilford Aves, Baltimore Md. 21202, USA. Mulia, D.S. & Purbomartono. 2007. Perbandingan efikasi vaksin produk intra dan ekstraseluler Aeromonas hydrophila untuk menanggulagi penyakit motile aeromonas septicemia (MAS) pada lele dumbo (Clarias sp.). Jurnal Perikanan 2: 173-181.

Noga, E.J. 2000. Fish disease diagnosis and treatment.Iowa State Press, Nort Carolina.

Puspowardoyo, H. & A.B. Djarijah. 1992. Membudidayakan gurami secara intensif. Penerbit Kanisius, Yogyakarta.

Roberts, R.J. 2001. Fish pathology. W. B. Saunders, Edinburgh. 472 p. Rollins, D.M.&S.W. Joseph. 2000. List of bacterial pathogen. BSCL 424 Pathogenic Microbiology. University of Maryland. Diakses tanggal 17 september 2010.

Rukmana, R. 2005. Pembenihan dan pembesaran gurami. Penerbit Kanisius, Yogyakarta. Sitanggang, M. & B. Sarwono. 2007. Budidaya gurami. Penebar Swadaya. Jakarta.

Stevenson, R.M.W. 1998. Vaccination againt Aeromonas hydrophila.In; Fish Vaccination. A.E. Ellis. (Ed). Academic Press, London p:112-123.

Zhang, Y. L., C.T. Ong, & K.Y. Leung. 2000. Molecular analysis of genetic differences between virulent and avirulent strains of Aeromonas hydrophila isolat from diseased fi sh. Microbiology. 146: 999 – 1009.



DOI: https://doi.org/10.22146/jfs.9102

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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