* Corresponding Author
Survey and Detection of Pectobacterium atrosepticum in Major Potato-Growing Areas in Central Java Province, Indonesia
https://doi.org/10.22146/ipas.11654 Ismiyatuningsih Ismiyatuningsih(1), Tri Joko(2*), Sedyo Hartono(3)
(1) Agency for Agricultural Quarantine Class I Semarang
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Potato (Solanum tuberosum) is a seasonal shrub-tuber crop originated from sub-tropical area. Soft-rot is one of the most important diseases of potato. It can be caused by Pectobactorium atrosepticum, a pathogen within a status of quarantine plant pest A1 type I in Indonesia. The objective of this study was to know the incidence of potato soft rot disease and to detect P. atrosepticum in major potato-growing areas in Central Java Province by applying the serology method using DAS-ELISA technique. Survey of soft rot disease was carried out in some regencies in Central Java Province, i.e. Magelang, Banjarnegara, Wonosobo and Karanganyar. The field survey of potato plant in all the regencies indicated symptoms of stem rot which was black in color (blackleg) and foul-smelling, with disease incidence of about 10–90%. The laboratory testing showed that by applying DAS-ELISA method, P. atrosepticum was detected in samples collected from Pandean and Bagongan villages, district of Ngablak,Regency of Magelang, Central Java Province.
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Anonim. 2011. Petunjuk Teknis Pemantauan Daerah Sebar OP/OPTK. Balai Karantina Pertanian Kelas I. Semarang. Pp: 1-30.
Baharuddin, Kuswinanti T, Lamba SE. 2012. Percepatan Ketersediaan Benih Kentang Unggulan Melalui Introduksi Paket Bioteknologi Ramah Lingkungan di Kabupaten Toraja Utara. Dalam: Prosiding InSINas; 2012 Nov 29-30; Bogor (ID): Institut Pertanian Bogor. hlm 336–344.
Bowden, G.H.W. 1993. Serological Identification. Academic Press, London.
Burgess, G.W. 1995. Prinsip Dasar Elisa dan Variasi Konfigurasinya, dalam Teknologi Elisa dalam Diagnosis dan Penelitian. Ed.Graham W. Burgess. Yogyakarta: Gadjah Mada University Press. Hal 50-59.
Cooke, B.M., 2006. Disease Assessment and Yield Loss. In: Cooke, B.M., D.G. Jones and B. Kaye (eds.), The Epidemiology of Plant Diseases, 2nd edition, pp:
43–80. Springer, The Netherlands.
Czajkowski, R., M.C.M. Pérombelon, J.A. van Veen, J.M. van der Wolf. 2011. Control of blackleg and tuber soft rot of potato caused by Pectobacterium and Dickeya species: a review. Plant Pathology 60 (6): 999-1013.
Diallo, S. Latuar, X. Groboillot, A. Smadja, B. Copin, P. Orange, N. Feuilloley, J. Chevaliner, S. 2009. Simultaneous and Selective Detection of Two Major Soft Rot Pathogens of Potato; Pectobactorium atrosepticum (Erwiniacarotovora subsp. atrosepticum) and Dickeya spp. (Erwinia chrysanthemi). Eur. Journal of Plant Pathol. 125 : 349-354.
Haerani, A.N. Nawangsih, T.A. Damayanti. 2015. Deteksi dan Identifikasi Dickeya sp. Sebagai Organisme Penganggu Tumbuhan Karantina A2 pada tanaman kentang di Jawa. Jurnal Fitopatologi Indonesia 11 (4): 105-112.
Joko, T., H. Hirata, S. Tsuyumu. 2007a. Sugar transporter (MfsX) of major facilitator superfamily is required for flagella-mediated pathogenesis in Dickeya dadantii 3937. Journal of General Plant Pathology 73: 266-273.
Joko, T., H. Hirata, S. Tsuyumu. 2007b. A sugar transporter (MfsX) is also required by Dickeya dadantii 3937 for in planta fitness. Journal of General Plant Pathology 73:274-80.
Joko, T., N. Kusumandari, S. Hartono. 2011a. Optimasi Metode PCR untuk Deteksi Pectobacterium carotovorum, Penyebab Penyakit Busuk Lunak Anggrek. Jurnal Perlindungan Tanaman Indonesia, 17 (2): 54-59.
Joko T, Kiswanti D, Hanudin, Subandiyah S. 2011b. Occurence of bacterial soft-rot of Phalaenopsis orchids in Yogyakarta and West Java, Indonesia. Proceeding of Internasional Seminar on “Natural Resources, Climate Change,danFood Security in Developing Countries”. 27-28 June 2011. Surabaya, Indonesia. P. 255-265.
Joko, T., M.P. Koentjoro, S. Somowiyarjo, M.S. Rohman, A. Liana, N. Ogawa. 2012. Response of Rhizobacterial Communities in Watermelon to Infection with Cucumber Green Mottle Mosaic Virus as Revealed by Cultivation-Dependent RISA. Archives of Phytopathology and Plant Protection, 45 (15): 1810-1818.
Joko, T., A. Subandi, N. Kusumandari, A. Wibowo, A. Priyatmojo. 2014. Activities of Plant Cell Wall Degrading Enzymes by Bacterial Soft-rot of Orchid. Archives of Phytopathology and Plant Protection, 47 (10): 1239-1250.
Perombelon, M.C.M. 1992. Potato Blackleg: Epidemology, Host-Pathogen interaction and Control. Netherland Journal of Plant Pathology, 98: 135-146.
Perombelon M.C.M.and Kelman A. 1980. Ecology of the soft rot erwinias. Annu Rev Phytopathol 18:361-367.
Toth I.K., Bell K.S., Holeva M.C., Birch P.R.J. 2003 Soft rot erwiniae: from genes to genomes. Mol Plant Path 4:17-30.
Wardhika, C.M., Suryanti, T. Joko. 2014. Eksplorasi Bakteri Agens Pengendali Hayati Fusarium solani dan Meloidogyne incognita pada Lada. Jurnal Perlindungan Tanaman Indonesia, 18 (2): 90-95.
DOI: https://doi.org/10.22146/ipas.11654
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