Growth and yield performance of four generations of high amylose transgenic Adira 4 cassava

Enny Sudarmonowati(1*), Hani Fitriani(2)

(1) The Indonesian Institute of Sciences Jl. Jenderal Gatot Subroto 10, Jakarta 12710
(2) The Indonesian Institute of Sciences Jl. Jenderal Gatot Subroto 10, Jakarta 12710
(*) Corresponding Author


Efforts to improve cassava starch quality have been conducted by producing the first high amylose transgenic Adira 4 Indonesian cassava in the world. To assess growth and yield performances of those plants derived from  propagated cuttings, improved techniques by assessing four generations grown in specific containment screenhouse  and limited field trial were conducted. The survival and growth rate of cuttings was enhanced by applying growth regulator i.e. Atonik and improved planting procedures. The growth and yield performances of this Adira 4 bearing GBSSI gene were measured by comparing related variables across four generation of selected lines grown in the biosafety screehouse and limited field trial in Indonesia. Dipping in 1 ml.L-1 Atonik solution prior to and  shading in the early planting were only required if the growth was poor and the soil and climate conditions were not favourable. Although shading and Atonik did not give significant different in most lines and generations, survival of third and fourth generation of Lines A55-5 and A55-15 could reach 100% if treated with Atonik. Of 14 lines assessed, line A55-5 was one of the promising one due to its growth, multiplication performance and  amylose content which was almost double  as compared to wild type, while Line A55-12 has the highest yield. Staining with lugol indicated that not only root storage or tubers stained dark blue but also the basal part of stems. 


Indonesia cassava variety; Adira 4; transgenic plants; high amylose; limited field trial; BE1 genes


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