ROLE OF REDUCING CELL LEAKAGE IN CELL CULTURE USING LARGE PORE SIZE PERMEABLE MEMBRANE
Nur Kaliwantoro(1*), Marsetyawan HNE Soesatyo(2), Indarto Indarto(3), Mohammad Juffrie(4), Rini Dharmastiti(5), Suprihatin Suprihatin(6)
(1) Histology and Cells Biology Department-Medical Faculty, Universitas Gadjah Mada
(2) Histology and Cells Biology Department-Medical Faculty Universitas Gadjah Mada
(3) Mechanical Engineering Department, Engineering Faculty Universitas Gadjah Mada
(4) Pediatric Department, Medical Faculty Universitas Gadjah Mada
(5) Industrial Engineering Department, Engineering Faculty Universitas Gadjah Mada
(6) Parasitology Laboratory, Medical Faculty Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Permeable membranes are widely used in many in vitro studies using cell culture. Some cell leakage are often occurs when using permeable membrane with large pore size. Unfortunately the use of permeable membrane with smaller pore size in permeability studies faces some difficulties due to its small pore size and pore density. Recent study provides the protocol in using permeable membrane with large pore size with smaller cell leakage. Vero cell line (CCL-81, ATCC) was used and culture on polyester permeable membrane with 3 µm pore size. Visualization using inverted microscope was used to analized the cell leakage on the permeable membrane. Parallel plate flow chamber was used to analized the permeability performance of the Vero cell cultured on the permeable membrane. The result showed that the current technique is significant in reducing cell leakage of the cell cultured on large pore size of permeable membrane. The same results were found in using polyester and polycarbonate permeable membrane.
Keywords
Full Text:
PDFReferences
Arslan N, Isik S, Uykan O, 2010, “Steady and disturbed flow effects on human umbilical vein endothelial cells (HUVECs) in vascular system: an experimental study”, Acta of Bioengineering and Biomechanics, 12 (4): 3-9.
Casnocha, 1990, “Permeability of endothelial monolayers under stationary and flow condition”, Dissertation, Michigan University, USA.
Corning, 2010, Corning Cell Culture Selection Guide.
Wahyudi, 2006, “Pengaruh Shear Stress terhadap sintesa dan release Nox dari HUVEC yang dipapar dengan Glukosa Suprafisiologis”,Disertasi, Universitas Brawijaya.
Yen YT, Chen HC, Lin YD, Shieh CC, Wu-Hsieh BA, 2008, “Enhancement by tumor necrosis factor alpha of dengue virus-induced endothelial cell production of reactive nitrogen and oxygen species is key to hemorrhage development”, Journal of virology. 82:12312–12324.
DOI: https://doi.org/10.22146/teknosains.27192
Article Metrics
Abstract views : 2088 | views : 1854Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Nur Kaliwantoro, Marsetyawan HNE Soesatyo, Indarto Indarto, Mohammad Juffrie, Rini Dharmastiti, Suprihatin Suprihatin
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Copyright © 2024 Jurnal Teknosains Submit an Article Tracking Your Submission
Editorial Policies Publishing System Copyright Notice Site Map Journal History Visitor Statistics Abstracting & Indexing