Comparing P-Selectin (CD62P) expression in patients receiving non-leukodepleted vs leukodepleted thrombocyte concentrates

https://doi.org/10.19106/JMedSci004903201704

Teguh Triyono(1*), Budi Mulyono(2), . Sutaryo(3), Abdul Salam Sofro(4)

(1) Departement of Clinical Pathology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
(2) Departement of Clinical Pathology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
(3) Departement of Pediatry, Faculty of Medicine, Universitas Gadjah Mada,
(4) Faculty of Medicine, Universitas YARSI, Jakarta, Indonesia
(*) Corresponding Author

Abstract


Thrombocyte concentrate (TC) transfusion is an important supportive therapy in patients with thrombocytopenia. The risks in platelet transfusions may be related to the content of TC including the contaminant leukocytes. The aim of this study was to assess the risk of increased level of P-Selectin (CD62P) expression of non-leukodepleted TC transfusions.This was a quasi-experimental study. Subjects were children patients aged 1-18 years who received a non-leukodepleted or a leukodepleted TC transfusions. Comparison of the proportion of  increased expression of CD62P in both groups expressed as relative risk. The subjects consisted of 51 patients who received non-leukodepleted and 52 patients who received leukodepleted TC transfusions. The risk of increased expression of CD62P in patients receiving non-leukodepleted TC transfusions were 2.38 (95%CI:1.60-3.53) times higher than those who received leukodepleted TC. Non-leukodepleted have significant higher risks of increased CD62P expression than leukodepleted  TC transfusions.

Keywords


thrombocyte conentrate – transfusion – CD62P expression - thrombocytopenia – adverse effect

Full Text:

PDF


References

Kemenkes RI . Laporan hasil UTD tahun 2014: Pelayanan darah di Indonesia. 2015. 2. UPTD RSUP Dr. Sardjito. Laporan pengeluaran darah dan rekapitulasi pengeluaran darah. Yogyakarta. 2011 & 2015. 3. Kaufman, R.M., Djulbegovic, B., Gernsheimer, T., Kleinman, S., Tinmouth, A.T., Capocelli, K.E., et al. Platelet transfusion: A clinical practice guideline from the AABB. Annals of Internal Medicine. [Online] 2015; 162 (3), 205–213. Available from: doi:10.7326/M14-1589. 4. Xie, Z.T., Chen, C., Zhang, S.H., Yang, H.M. & Tao, Z.H. Effect of leukocyte filtration on the P-selectin expression of apheresis platelets. Genetics and Molecular Research. [Online] 2015;14 (2), 5979–5985. Available from: doi:10.4238/2015.June.1.15. 5. Sharma, RR., Marwaha,N. Leukoreduced blood components: Advantages and strategies for its implementation in developing countries. Asian Journal of Transfusion Science. 2010; 4, 3-8. 6. Nielsen,H.J.,Reimert,C.M.,Pedersen,A.N.,Brunner,N.,Edvardsen,L.,Dybkler,L.,Kehlet,H., Skov,P.S. Time-dependent, spontaneous release of white cell and platelet-derived bioactive substances from stored human blood. Transfusion. 1996; 36, 960-965. 7. Faraday, N., Scharph, R.B., Dodd-o, J.M., Martinez, E.A., Rosenfeld, B.A., Dorman, T. Leukocytes can enhance platelet-mediated aggregation and thromboxane release via interaction of P-selection glycoprotein ligand 1 with P-selectin. Anesthesiology. 2001; 94, 145-151. 8. Faint, R.W. Platelet-Neutrophil Interactions: their significance. Blood. 1992; 6, 83-91 9. Mcfaul,S.J., Corley,J.B., Mester,C.W., Nath,J. Packed blood cells stored in AS-5 become proinflammatory during storage. Transfusion. 2009; 49, 1451-1460. 10. Kolarova, H., Klinke, A., Kremserova, S., Adam, M., Pekarova, M., Baldus, S., Eiserich, J., P., Kubala, L. Myeloperoxidase induces the priming of platelets. Free Radical Biology and Medicine. 2013; 61, 357-369. 11. Eiserich J.P., Baldus S., Bernnan M.L., Ma W., Zhang C., Tousson A., Castro L., Lussis A.J., Nauseef W., White R. & Freeman B.A. Myeloperoxidase a leukocyte-derived vascular NO oxidase. Science. 2002; 296, 2391–2394. 12. Baldus,S., Heitzer, T., Eiserich, J.P., Lau, D., Mollnau, H., Ortak, M., Petri, S., Goldmann, B., Duchstein, H.J., Berger, H., Helmchen, U., Freeman, B.A., Meinertz K.T.,Munzel,T. Myeloperoxidase enhances nitric oxide catabolism during myocardial ischemia and reperfusion. Free Radical Biology and Medicine. 2004; 37, 902–911. 13. Kim,S.W.,Lim,Y.A. Establishment of reference values for platelet activation markers by flowcytometry. Korean Journal of Laboraatory Medicine. 2006; 26, 323-328. 14. Nomura, S., Okamae, F., Abe, M., Hosokawa, M., Yamaoka, M., Ohtani, T., et al. Platelets expressing P-selectin and platelet-derived microparticles in stored platelet concentrates bind to PSGL-1 on filtrated leukocytes. Clinical and applied thrombosis/hemostasis. [Online] 2000; 6 (4), 213–221. Available from: doi:10.1177/107602960000600406. 15. Plaza, E.M., Cespedes, P., Fernandez, H., Sanchez-Guiu, M.I., Egea, J.M., Vicente, V., et al. Quality assessment of buffy-coat-derived leukodepleted platelet concentrates in PAS-plasma, prepared by the OrbiSac or TACSI automated system. Voxsanguinis. 2013; 106(1), pp.1–7. 16. Bashir, S., Nightingale, M.J.& Cardigan, R. Ensuring that blood transfusion sets an effective dose of functional blood components. Transfusion medicine. 2013; 23(4), 226–30. 17. Nagy, B.J., Debreceni, I.B. &Kappelmayer, J. (2012)Flowcytometryin the clinical laboratory flowcytometric investigation of classical and alternative platelet activation.The Journal of the International Federation of Clinical Chemistry and Laboratory Medicine.23(4), pp.1–11. 18. Vetlesen, A., Holme, P.A., Lyberg, T. & Kjeldsen-Kragh, J. Recovery, survival, and function of transfused platelets and detection of platelet engraftment after allogeneic stem cell transplantation. Transfusion. [Online] 2012; 52 (6), 1321–1332. Available from: doi:10.1111/j.1537-2995.2011.03442.x. 19. Ritchie, J.L., Alexander, H.D., Rea, I.M. Flow cytometry analysis of platelet P-selectin expression in whole blood-methodological considerations. Clinical & Laboratory Haematology. 2000; 22(6), pp. 359-363. 20. Horn, M., Bertling, A., Brodde, M.F., Muller, A., Roth, J., Van Aken, H., et al. Human neutrophil alpha-defensins induce formation of fibrinogen and thrombospondin-1 amyloid-like structures and activate platelets via glycoprotein IIb/IIIa. Journal of Thrombosis and Haemostasis. [Online] 2012; 10 (4), 647–661. Available from: doi:10.1111/j.1538-7836.2012.04640.x. 21. Levin, E., Jenkins, C., Culibrk, B., Gyöngyössy-Issa, M.I.C., Serrano, K. & Devine, D. V. Development of a quality monitoring program for platelet components: A report of the first four years’ experience at Canadian Blood Services. Transfusion. [Online] 2012; 52 (4), 810–818. Available from: doi:10.1111/j.1537-2995.2011.03402.x. 22. Panzer, S. & Jilma, P. Methods for testing platelet function for transfusion medicine. Voxsanguinis. 2011; 101(1), pp.1–9.



DOI: https://doi.org/10.19106/JMedSci004903201704

Article Metrics

Abstract views : 2800 | views : 2058


Bookmark and Share


Copyright (c) 2017 Teguh Triyono, Budi Mulyono, . Sutaryo, Abdul Salam Sofro

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.