Expression of circulating miR-200c and vascular endothelial growth factor-A (VEGF-A) mRNA as potential biomarker in human hepatocellular carcinoma
https://doi.org/10.19106/JMedScie/005004201805
Nanda Qoriansas
(1*) , Dede Renovaldi
(2) , Juwita Raditya
(3) , Puji Lestari
(4) , Nur Signa Gumilas
(5) , . Suharno
(6) , Didik Setyo Heriyanto
(7) , Neneng Ratnasari
(8) , Sofia Mubarika H
(9)
(1) Basic Medical Science and Biomedicine Program Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(2) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(3) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(4) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(5) Faculty of Medicine, UNSOED Purwokerto Indonesia
(6) Faculty of Medicine, UNSOED Purwokerto Indonesia
(7) Department of Anatomic Pathologiy, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(8) Department of Internal Medicine, Universitas Gadjah Mada Yogyakarta
(9) UGM Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(*) Corresponding Author
Abstract
Hepatocellular carcinoma (HCC) is a common liver disease that causes significant public health problems throughout the world, including in Indonesia. The HCC is the six most common cancers and second cancer-related deaths among men in the world. Recently it was reported that the microRNA is an important player in hepatocarcinogenesis. The expression of MiRNA-200c is often regulated in primary HCC and HCC cell lines. Vascular endothelial growth factor-A (VEGF-A) is a regulator of angiogenesis that has been reported as miR-200c target gene. This study was conducted to measure expression levels in miR- 200c and mRNAVEGF-A and their potential role as biomarkers at HCC. A total of 36 HCC patients and 36 healthy subjects were included in this study. The relative expression of miRNA-200c and mRNA VEGF-A was quantified using reverse transcription real time quantitative PCR (qRT PCR). Relative expression was calculated using . Unpaired t-test was used to compare the expression levels of circulating miRNA-200c and mRNA VEGF-A in HCC patients and healthy subjects. Pearson test was used to determine correlation between circulating miR-200c expression and mRNA VEGF-A expression levels. The expression levels of circulating miR-200c in HCC patients were lower compared to healthy subjects although it was not significant (p = 0.258). Conversely, the expression levels of circulating mRNA VEGF-A in HCC patients were significantly higher compared to healthy subjects (p = 0.001). The relative expression levels of circulating miR-200c were negatively correlated with mRNA VEGF-A in HCC patients. In conclusion, the expression levels of mRNA VEGF-A in HCC patients are significantly deregulated in compared to that in healthy subjects. Negative correlation between circulating miRN-200c and mRNA VEGF-A expression levels are reported in HCC patients.
Keywords
blood plasma - hepatocellular carcinoma - miR-200c - mRNA VEGF-A - biomarker
DOI:
https://doi.org/10.19106/JMedScie/005004201805
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