Current in vitro assay to determine bacterial biofilm formation of clinical isolates

Abstract

Biofilm mode of growth is bacterial lifestyle widespread in microbial world and represents a concern
in health care. Recently, serious problem concerning biofilm-related infection is increasing significantly.
However complexity of biofilm makes it difficult to conduct proper analysis. Although biofilm represents
a major challenge for microbiologist, methods aimed to determine biofilm formation and development
are not standardized yet. The aim of the present review was to provide an overview of the advantages
and disadvantages of the difference in vitro methods for determining biofilm forming ability of
clinical isolates.