The effect povidone-iodine on the wound healing process: A study on fibroblast populated collagen lattice (FPCL) model
https://doi.org/10.19106/JMedScie.004603201401
Retno Danarti
(1*) , . Suswardana
(2) , Arief Budiyanto
(3) , Widodo Wirohadidjojo
(4)
(1) Department of Dermatology and Venereology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) dr. mintohardjo Navy Hospital, Jakarta
(3) Department of Dermatology and Venereology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(4) Department of Dermatology and Venereology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(*) Corresponding Author
Abstract
Povidone-iodine (PI) 10% solution is an effective antiseptic. However, it appears to be toxic to the cells involved in wound healing. The aim of this study is to evaluate the toxicity of PI on cultured human fibroblast using fibroblast populated collagen lattice (FPCL) model. The cultured human fibroblast was divided into 6 groups i.e. 5 groups were exposed by PI 1, 0.1, 0.01, 0.001 and 0.0001%, and 1 group was exposed by phosphate-buffered-saline (PBS). Twenty-four hours later, the media was washed using PBS. The size of the FPCL media on each group was observed over time by serial photographs, which then were measured by Image-J computer program. Exposure of 0.1, 0.01, 0.001 and 0.0001 PI caused an obvious reduction of fibroblast’s contraction capability on FPCL media, which described temporary fibroblast injury, that showing a concentration-dependent recovery phenomenon after 48th hour. Furthermore, 1% PI exposure leads to a permanent fibroblast injury. In conclusion, PI exposure in concentration more than 0.1% has a permanent toxic effect on fibroblast that clearly observed using a simple FPCL model.
Keywords
fibroblast - povidone-iodine - toxicity - wound-healing – fibroblast injury
DOI:
https://doi.org/10.19106/JMedScie.004603201401
Article Metrics
Abstract views : 2675
|
views : 2773
Copyright (c) 2015 Retno Danarti, . Suswardana, Arief Budiyanto, Widodo Wirohadidjojo
This work is licensed under a
Creative Commons Attribution-NonCommercial 4.0 International License .
<div class="statcounter"><a title="real time web analytics" href="https://statcounter.com/" target="_blank"><img class="statcounter" src="https://c.statcounter.com/10697571/0/23d82b78/0/" alt="real time web analytics"></a></div> <div class="statcounter"><a title="shopify visitor statistics" href="http://statcounter.com/shopify/" target="_blank"><img class="statcounter" src="http://c.statcounter.com/10697571/0/23d82b78/0/" alt="shopify visitor statistics"></a></div>
View My Stats
Journal of the Medical Sciences (Berkala Ilmu Kedokteran ) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License . Based on a work at http://jurnal.ugm.ac.id/bik/ .