The effect of active compound isolated from the leaves of kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line

https://doi.org/10.19106/JMedScie004503201301

Hajid Rahmadianto Mardihusodo(1*), Mae Sri Hartati Wahyuningsih(2), Indwiani Astuti(3)

(1) Postgraduate Program in Biomedical Sciences, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Colorectal cancer is the tenth most common form of malignant tumor of hospital inpatients in
Indonesia. Advance approaches in anticancer development is discovery molecular-targeted drugs.
Molecular targets for anticancer drug have been identified including genes associated with cell
cycle control and angiogenesis. Previously, an active and selective compound against WiDr from
Tithonia diversifolia (Hemsley) A. has been isolated. The aim of this study was to evaluate the
effect of the isolated active compound fromT. diversifolia on the WiDr cell cycle and angiogenesis.
Isolation of the active compound was performed by preparative thin layer chromatography (TLC)
method. WiDr cell cycle was analyzed by flowcytometry using propidium iodide (PI).
Antiangiogenesis effect was evaluated by immunocytochemistry method using anti-human VEGF
monoclonal antibody. The results showed that the effect of the isolated active compound on
the WiDr cell cycle depended on the concentration and the incubation time periods. At
concentration of 4 μg/mL, it inhibited the WiDr cell cycle SubG1 phase after 36 and 48 hours
incubation and G1 phase after 72 hours incubation. While at concentration of 8 μg/mL, it clearly
inhibited the WiDr cell cycle G1 phase after 36, 48 and 72 hours incubation. Furthermore, the
isolated active compound at concentration of 4 μg/mL significantly inhibited the VEGF expression
until 47.38% compared to control. In conclusion, the isolated active compound fromT. diversifolia
inhibited cell cycle and angiogenesis of WiDr cell.

Keywords


isolated active compound – T. diversifolia - WiDr cells - cell cycle – antiangiogenesis

Full Text:

PDF



DOI: https://doi.org/10.19106/JMedScie004503201301

Article Metrics

Abstract views : 401 | views : 592




Copyright (c) 2015 Journal of the Medical Sciences



View My Stats

 

Creative Commons License
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by  Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Based on a work at http://jurnal.ugm.ac.id/bik/.