Identification of Conjugated Linoleic Acid in Milk Fermented by Probiotics Originating in the Gastrointestinal Tract

https://doi.org/10.21059/buletinpeternak.v43i2.17325

Kafaah Estancia(1), Widodo Widodo(2*)

(1) 
(2) Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract


One of the benefits of probiotics is their ability to synthesize conjugated linoleic acid (CLA) as a functional bioactive compound. The objective of this study is to identify CLA synthesized in milk fermented with the probiotic Lactobacillus casei strain AP and Lactobacillus casei strain AG as starter cultures. Fermented milk products were analyzed using gas chromatography-mass spectrometry (GC-MS) to characterize the CLA formed. The result of GC-MS in milk fermented using Lactobacillus casei strain AG was detection of a CLA compound with a retention time of 41.467, whereas in milk fermented using Lactobacillus casei strain AP, linoleic acid, but not CLA, was detected.

Keywords


CLA; Fermented milk; Lactobacillus casei strain AG; Lactobacillus casei strain AP; Probiotic

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References

Alonso, L., E. P. Cuesta, and S. E Gilliand. 2003. Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin. J. Dairy Sci. 86: 1941-1946. https://doi.org/10.3168/jds.S0022-0302(03)73781-3.

Heimbach, J. T. 2012. Generally recognized as safe (GRAS) determination for the use of Lactobacillus casei strain Shirota as a food ingredient. GRAS Notice No 000429. Yakult Honsha Co., Japan.

Kishino, S., M. Takaeuchi, S. B. Park, A. Hirata, N. Kitamura, J. Kunisawa, H. Kiyono, R. Iwamoto, Y. Isobe, M. Arita, H. Arai, K. Ueda, J. Shima, S. Takashi, K. Yokozeki, S. Shimizu, and J. Ogawa. 2013. Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition. Proc. Natl. Acad. Sci. 110: 17808-17813. https://doi.org/10.1073/ pnas.1312937110.

Makinen, A. M. and M. Bigret. 2004. Industrial use and productions of lactic acid bacteria. Marcel Dekker, Inc, New York.

Parodi, P. W. 1999. Conjugated linoleic acid and other anticarcinogenic agents of bovine milk fat. J Dairy Sci. 82:1339-1349. https://doi.org/10.3168/jds.S0022-0302(99)75358-0.

Ross, R. P., S. Mills, C. Hill, G. F. Fitzgerald, and C. Stanton. 2010. Specific metabolite production by gut microbiota as a basis for probiotic function. J. Dairy Sci. 20: 269-276. https://doi.org/10.1016/j.idairyj. 2009.12.003.

Soeparno. 2015. Properti dan teknologi produk susu. Gadjah Mada University Press, Yogyakarta.

Tamime, A. Y. 2006. Fermented milks. Blackwell Publishing Company, USA.

Widodo. 2003. Teknologi Proses Susu Bubuk. Lacticia Press, Yogyakarta.

Widodo, T. T. Taufiq, E. Aryati, A. Kurniawati, and W. Asmara. 2012a. Human origin Lactobacillus casei isolated from Indonesian infants demonstrating potential characteristics as probiotics in vitro. Indonesian Journal of Biotechnology. 17: 79-89. https://doi.org/10.22146/ ijbiotech.7852.

Widodo, N. S. Anindita, T. T. Taufiq, and T. D. Wahyuningsih. 2012b. Identification of Pediococcus strains isolated from feces of Indonesian infants with in vitro capability to consume prebiotic inulin and to adhere on mucus. Indonesian Journal of Biotechnology. 17: 132-143. https://doi.org/10.22146/ijbiotech.7859.

Widodo, R. Handaka, E. Wahyuni, and T. T. Taufiq. 2017. The quality of fermented milk produced using intestinal-origin lactic acid bacteria as starters. Int. Food Res. J. 24: 2371-2376.



DOI: https://doi.org/10.21059/buletinpeternak.v43i2.17325

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