Utilization of Gamma Irradiated Aspergillus niger to Improve Oil Palm by-Product Digestibility

https://doi.org/10.21059/buletinpeternak.v42i1.27706

Teguh Wahyono(1*), Nana Mulyana(2), Putri Amanda(3), Siti Nurbayti(4), Suharyono Soharyono(5)

(1) Field of Agriculture, Center for Application of Isotope and Radiation, National Nuclear Energy Agency of Indonesia, South Jakarta, 12070, Indonesia
(2) Field of Industry and Environment, Center for Application of Isotope and Radiation, National Nuclear Energy Agency of Indonesia , South Jakarta, 12070, Indonesia
(3) Faculty of Science and Technology, Syarif Hidayatullah State Islamic University, Banten, 15419, Indonesia
(4) Faculty of Science and Technology, Syarif Hidayatullah State Islamic University, Banten, 15419, Indonesia
(5) Field of Agriculture, Center for Application of Isotope and Radiation, National Nuclear Energy Agency of Indonesia, South Jakarta, 12070, Indonesia
(*) Corresponding Author

Abstract


This study was conducted to determine the effect of fermentation using irradiated A. Niger on in vitro rumen fermentation characteristics of oil palm by-products. Completely randomized design with eight treatments and four replications was applied in this experiment. The treatments were kernel shell (CK), palm frond (PKS), oil palm empty bunches (TKKS), CK+PKS+TKKS (mix), fermented kernel shell (CKF), fermented palm frond (PKSF), fermented oil palm empty bunches (TKKSF) and fermented mix (mixF). The results showed that fermentation process reduced NDF content of PKS and TKKS by 7.42 and 7.09% respectively. Fermentation also decreased ADF content of TKKS by 7.35%. Maximum total gas production (a+b) of TKKS and mix sample decreased after fermentation process (P<0.05) by 52.92 and 35.60% respectively. Total VFA production increased on kernel shell and palm fronds samples after fermentation process (P<0.05) however CO2:CH4 ratio tended to be decrease. The conclusion of this study was the fermentation process by irradiated A. niger improved rumen fermentation characteristics of oil palm by-products, however more appropriate strategy is needed to reduce CH4 emissions.


Keywords


Aspergillus niger; Degradability; Fermentation; Gamma irradiation; Oil palm by-products

Full Text:

PDF


References

Akbar, S. A. 2007. Pemanfaatan tandan kosong sawit fermentasi yang dikombinasikan dengan defaunasi dan protein by pass rumen terhadap performans ternak domba. J. Indon. Trop. Anim. Agric. 32: 80-85.

Antonius, K. G. Wiryawan, A. Thalib and A. Jayanegara. 2015. Digestibility and methane emission of ration based on oil palm by products supplemented with probiotics and banana stem. Pakistan J. Nut. 14: 37-43. doi: http://doi.org/10.3923/pjn.2015.37.43.

AOAC. 2010. Official methods of analysis of AOAC International. 18th Edition. Revision 3. Association of Official Analytical Chemist, Washington DC.

Badan Pusat Statistik (BPS). 2016. Statistik Indonesia. Badan Statistik Indonesia. Jakarta.

Baker, S. K. 1999. Rumen methanogenes and inhibition of methanogenesis. Aust. J. Agric. Res. 50: 1293-1298.

Blümmel, M., H. Steingass and B. Becker. 1997. The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporated and its implication for the prediction of voluntary feed intake of roughages. Br. J. Nutr. 77: 911-921.

Cardozo, P.W., S. Calsamiglia and A. Ferret. 2000. Effects of pH and pH fluctuations on microbial fermentation and nutrient flow in a dual flow continuous culture system. J. Dairy Sci. 83 (Suppl. 1): 265.

Chanjula, P., A. Mesang and S. Pongprayoon. 2010. Effects of dietary inclusion of palm kernel cake on nutrient utilization, rumen fermentation characteristics and microbial populations of goats fed Paspalum plicatulum hay-based diet. Songklanakarin J. Sci. Technol. 32: 527-536.

Conway, E. J. 1950. Micro-diffusion Analysis and Volumetric Error. Lockwood. London.

Fariani, A., A. Abrar dan G. Muslim. 2013. Kecernaan pelepah sawit fermentasi dalam Complete Feed Block (CFB) untuk sapi potong. J. Lahan Subobtimal 2: 129-136.

Getachew, G., M. Blümmel, H. P. S. Makkar and K. Becker. 1998. In vitro gas measuring techniques for assessment of nutritional quality of feeds: a review. Anim Feed. Sci. Technol. 72: 261-281.

Goering, H. K. and P. J. Van Soest. 1970. Forage Fibre Analysis (apparatus, reagents, procedures, and some application). Agric. handbook 379, ARS., USDA., Washington, DC. USA.

Islam, M., I. Dahlan, A. Rajion and Z. A. Jelan. 2000. Productivity and nutritive values of different fractions of oil palm (Elaeis guineensis) frond. Asian-Aus. J. Anim. Sci. 13: 1113-1120. doi: https://doi.org/10.5713/ajas.2000.1113.

Jahromi, M. F., J. B. Liang, M. Rosfarizan, Y. M. Goh, P. Shokryazdan and Y. W. Ho. 2010. Effects of Aspergillus niger (K8) on nutritive value of rice straw. Afr. J. Biotechnol. 9: 7043-7047.

Jayanegara, A., H. P. S. Makkar and K. Becker. 2009. Emisi metana dan fermentasi rumen in vitro ransum hay yang mengandung tanin murni pada konsentrasi rendah. Med. Pet.. 32: 184-194. https://doi.org/10.5398/medpet.v32i3.1131.

Kaur, K., M. Wadhwa and M. P. S. Bakshi. 2010. Nutritional evaluation of Pleurotus florida harvested spent wheat-rice straw based diets in goats. Indian J. Anim. Sci. 80: 906-909.

Li, X. X., Z. Durmic, S. M. Liu, C. S. McSweeney and P. E. Vercoe. 2014. Eremophila glabra reduces methane production and methanogen populations when fermented in a Rusitec. Anaerobe 29: 100–107.

Mahesh, M. S. 2012. Fungal bioremediation of wheat straw to improve the nutritive value and its effect on methane production in ruminants. MVSc thesis submitted to National Dairy Research institute (Deemed University), Karnal, Haryana, India.

Mahesh, M. S. and M. Mohini. 2013. Biological treatment of crop residues for ruminant feeding: a review. Afr. J. Biotechnol. 12: 4221-4231. https://doi.org/10.5897/AJB2012.2940.

Martinez, M. E., M. J. Ranilla, M. L. Tejido, C. Saro and M. D. Carro. 2010. Comparison of fermentation of diets of variable composition and microbial populations in the rumen of sheep and Rusitec fermenters. I. Digestibility, fermentation parameters, and efficiency of microbial protein synthesis. J. Dairy Sci. 93: 3684–3698. https://doi.org/10.3168/jds.2009-2933.

Menke, K. H., L. Raab, A. Salewski, H. Steingass, D. Fritz and W. Schneider. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor. J. Agric. Sci. 93: 217-222.

Mulyana, N., T. R. D. Larasati, Nurhasni dan M. Ningrum. 2015. Peningkatan aktivias enzim selulase dan produksi glukosa melalui fermenasi substrat jerami padi dengan fungi Aspergillus niger yang dipapar sinar gamma. Jurnal Ilmiah Aplikasi Isotop dan Radiasi. 11: 13-26.

Okoroigwe, E. C., C. M. Saffron and P. D. Kamdem. 2014. Characterization of palm kernel shell for materials reinforcement and water treatment. JCEMS 5: 1-6. https://doi.org/10.5897/JCEMS2014.0172.

Ørskov, E. R. and I. Mcdonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to the rate of passage. J. Agric. Sci. Camb. 92: 499-503.

Puastuti, W., D. Yulistiani dan I. W. R. Susana. 2014. Evaluasi nilai nutrisi bungkil inti sawit yang difermentasi dengan kapang sebagai sumber protein ruminansia. JITV 19: 143-151.

Rahman, M. M., M. Lourenco, H. A. Hassim, J. J. P. Boars, A. S. M. Sonnenberg, J. W. Cone, J. De Boever and V. Fievez. 2011. Improving ruminal degradability of oil palm fronds using white rot fungi. Anim. Feed. Sci. Technol. 169:157-166. doi: https://doi.org/10.1016/j.anifeedsci.2011.06.014.

Saka, S., V. Munusamy, M. Shibata, Y. Tono and H. Miyafuji. 2008. Chemical constituents of the different anatomical parts of the oil palm (Elaeis guineensis) for their sustainable utilization. JSPS-VXX group seminar, Natural Resources and Energy Environment 24-25 November 2008, Kyoto, Japan, 19-34.

Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedures of Statistics, 2nd ed., McGraw-Hill., New York.

Van Soest, P. J. 1982. Nutritional Ecology of Ruminant. Ruminant Metabolism, Nutritional Strategies, The Cellulolytic Fermentation and The Chemistry of Forages and Plant Fibers. Cornell University.

Wahyono, T. 2015. Evaluasi Fermentabilitas Ransum Kerbau yang Mengandung Sorgum dengan Pendekatan In Sacco, In Vitro dan RUSITEC. Thesis. Institut Pertanian Bogor.

Wahyono, T., D. A. Astuti, K. G. Wiryawan and I. Sugoro. 2015. Methane production and rumen fermentation characteristics of buffalo ration containing sorghum silage with rumen simulation technique (RUSITEC). The 6th International Seminar on Tropical Animal Production, October 20-22, Yogyakarta, Indonesia, 138-142.

Wajizah, S., Samadi, Y. Usman dan E. Mariana. 2015. Evaluasi nilai nutrisi dan kecernaan in vitro pelepah kelapa sawit (oil palm fronds) yang difermentasi menggunakan Aspergillus niger dengan penambahan sumber karbohidrat yang berbeda. Agripet. 15: 13-19.

Zahari, W. M., A. R. Alimon and H. K. Wong. 2012. Utilization of oil palm co-products as feeds for livestock in Malaysia. In Biofuel Co-Products as Livestock Feed Opportunities and Challenges. Food and Agriculture Organization of The United Nations, Rome, Italy, 243-302.

Wanapat, M., S. Kang and K. Phesatcha. 2013. Enhancing buffalo production efficiency through rumen manipulation and nutrition. Buffalo Bull. 32: 258-275.



DOI: https://doi.org/10.21059/buletinpeternak.v42i1.27706

Article Metrics

Abstract views : 2668 | views : 2614

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

   
 
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.