SRY Gene Marker Differences in Native and Crossbreed Cattle

Tety Hartatik; Dwi Ahmad Priyadi; Ali Agus; Sigit Bintara; I Gede Suparta Budisatria; Panjono Panjono; Budi Prasetyo Widyobroto; Yudi Adinata

doi:10.21059/buletinpeternak.v42i3.29940

SRY Gene Marker Differences in Native and Crossbreed Cattle

https://doi.org/10.21059/buletinpeternak.v42i3.29940

Tety Hartatik^(1*), Dwi Ahmad Priyadi⁽²⁾, Ali Agus⁽³⁾, Sigit Bintara⁽⁴⁾, I Gede Suparta Budisatria⁽⁵⁾, Panjono Panjono⁽⁶⁾, Budi Prasetyo Widyobroto⁽⁷⁾, Yudi Adinata⁽⁸⁾

(1) Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(2) Technology of Processing Livestock Product, Politeknik Negeri Banyuwangi. Jawa Timur, 68461, Indonesia
(3) Departement of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(4) Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(5) Departement of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada. Yogyakarta, 55281, Indonesia
(6) Departement of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada. Yogyakarta, 55281, Indonesia
(7) Departement of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada. Yogyakarta, 55281, Indonesia
(8) Beef Cattle Research Station, Grati, Pasuruan, 6714, Indonesia
(*) Corresponding Author

Abstract

This study focused on the promoter region of the SRY gene with 1,281 bp DNA fragments, including 5'UTR, CAAT signal, and TATA box. Genomic samples of 19 cattle were obtained from Wagyu-BX (n = 2), BX (n = 5), Simmental (n = 2), Limousin (n = 2), Ongole (n = 2), Madura (n = 2), Bali (n = 2), Nellore (n = 1), and Hereford (n = 1). Two flanking primers (forward and reverse) were used for polymerase chain reaction (PCR). The PCR products were then sequenced by using a two-way primer. The obtained sequences were aligned with clustalW software to determine the differences in the nucleotide base arrangement which compiled the promoter region of the SRY gene. The cattle crossbreeding was done as an effort to improve the genetic variations and qualities. The SRY gene is a marker gene inherited from the male side (bull), so the SRY gene is expected to be used as a marker to monitor the crossbreeding. The monitoring of the crossbreed cattle is an initial effort to increase the genetic variations and enhance the genetic qualities without threatening the germplasm purity. The results of this study showed that the overall sample is monomorphic, except for Bali and Nellore cattle. Further research is needed by expanding the analysis area of the SRY gene and increasing the number of samples.

Keywords

Genetics marker; PCR; Promoter region; Sequencing; Sex-determining region Y (SRY)

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DOI: https://doi.org/10.21059/buletinpeternak.v42i3.29940