Effect of Extender and Cooling Rate on the Quality of Frozen Thawed Semen of Bali Bull (Bos Sondaicus)

Anna Farhana; Zaituni Udin; Jaswandi Jaswandi; Riyan Nugroho Aji

doi:10.21059/buletinpeternak.v42i4.35173

Effect of Extender and Cooling Rate on the Quality of Frozen Thawed Semen of Bali Bull (Bos Sondaicus)

https://doi.org/10.21059/buletinpeternak.v42i4.35173

Anna Farhana^(1*), Zaituni Udin⁽²⁾, Jaswandi Jaswandi⁽³⁾, Riyan Nugroho Aji⁽⁴⁾

(1) Laboratory of Animal Reproductive Physiology, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(2) Laboratory of Production, Department of Animal Production,Faculty of Animal Science, University of Andalas, Padang, 25163, Indonesia
(3) Laboratory of Production, Department of Animal Production,Faculty of Animal Science, University of Andalas, Padang, 25163, Indonesia
(4) Laboratory of Animal Reproductive Physiology, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(*) Corresponding Author

Abstract

The purpose of this study was to observe the effect of extender and cooling rate on the quality of frozen thawed semen of Bali bull (Bos sondaicus). The experiment was conducted at Laboratory of Animal Biotechnology, Faculty of Animal Husbandry, Andalas University. A completely randomized design of factorial 2 x 3, 2 different extenders and 3 cooling rates with 3 bulls as the replicate. Data were analyzed using analysis of variance (ANOVA). The first factors was extender such as tris citrate egg yolk (TCEY) and tris citrate soy milk (TCSM). The second factors was the cooling rate such as: 15ºC/min, 10ºC/min and 5ºC/min. Variables were: motility, viability, abnormality, and membrane integrity. The results of treatment of TCEY extender on the average of motility 42.67±5.17%, viability of 53.78±3.79%, abnormality of 18.89±1.07%, and membrane integrity of 35.44±3.01%. The effect of cooling rate of 10°C /min has the highest semen motility of 44.35±5.28%, viability of 57.17±1.18%, abnormality of 17.84 ± 0.23 % and membrane integrity of 36.83±2.12%. The interaction between extender and cooling rate was founded significant different (P<0.05). Otherwise of extender and cooling rate on post-thawed of motility, viability and membrane integrity, had no difference (P>0.05) on semen abnormality. It can be concluded that tris-citrate egg yolk (TCEY) extender with cooling rate of 10ºC / min were the best semen motility, viability, and membrane integrity of spermatozoa.

Keywords

Bali bull; Cooling rate; Extender; Frozen semen

Full Text:

PDF

References

Ahmad, M., R. Nasrullah, H. Riaz, A. Sattar, and N. Ahmad. 2014. Changes in motility,morphology, plasma membrane and acrosome integrity during stage of cryopreservation of buck sperm. J. S. Afr. Assoc. 85: 1−4.

Aji, R. N., Panjono, A. Agus, B. P. Widyobroto, T. Hartatik, I. G. S. Budisatria, Ismaya, dan S. Bintara. 2017. Kinerja reproduksi sapi betina Sumba Ongole yang diinseminasi dengan semen beku sapi jantan Belgian Blue. Buletin Peternakan 41: 379−384.

Anton, M., F. Nau, and Y. Nys. 2005. Bioactive egg components and their potential uses. Paper : The XI th European Symposium on the quality of eggs and egg products, Doorwert. The Netherlands.

Arifiantini, R. I., T. L. Yusuf, and D. Yanti. 2005. Kaji banding kualitas sperma beku sapi Friesiean Holstein menggunakan pengencer dari berbagai balai inseminasi buatan di Indonesia. J. Anim. Prod. 7 : 168−176.

Belala, R., L. Briand-Amirat, L. Vinciguerra, D. Tainturier, R. Kaidi, C. Thorin, S.
Michaud, M. Anton, and D. Bencharif. 2016. Effect of equilibration time on
the motility and functional integrity of canine spermatozoa frozen in three
different extenders. Res. Vet. Sci. 106: 66–73.

Campbell, J. R., K. L. Campbell, and M. D. Kenealy. 2003. Anatomy and physiology of reproduction and related technologies in farm mammals In: Animal Science 4^th edn. New York, Mc Graw-Hill. pp.251–268.

Feradis. 2010. Bioteknologi Reproduksi pada Ternak. Penerbit Alfabeta Bandung Anggota Ikatan Penerbit Indonesia, Bandung.

Hafez, E. S. E. 2008. Preservation and cryopreservation of gamet and embryos in reproduction farm animal. Hafez E.S.E. and B. Hafez (eds) 7^th edn. Lippincott Williams & Wilkins. Marryland, USA. pp. 82–95.

Hendri, Z. Udin, dan Jaswandi. 2004. Bioteknolgi Reproduksi Ternak. Fakultas Peternakan, Universitas Andalas, Padang.

Ismaya. 2014. Bioteknologi inseminasi buatan pada sapi dan kerbau. Gadjah Mada University Press, Yogyakarta.

Mardiansyah, E. Yuliani, dan S. Prasetyo. 2016. Respon tingkah laku birahi, service per conception, non return rate, conception rate pada sapi Bali dara dan induk yang disinkronisasi birahi dengan hormon progesteron. Jurnal Ilmu dan Teknologi Peternakan Indonesia 2: 134–143.

Park, C. S. and V. G. Pursel. 1993. Effect of freezing rate on boar sperm frozen in maxi-straw. J. Anim. Sci. 61: 400–441.

Susilawati, T. 2013. Pedoman Inseminasi Buatan pada Ternak. Universitas Brawijaya (UB) Press, Malang.

Swelum, A. A., H. A. Mansour, A. A. Elsayed, and H. A. Amer. 2011. Comparing ethylene glycol with glycerol for cryopreservation of buffalo bull sperma in egg-yolk containing extenders. Theriogenology 76: 833–842.

Thun, R., M. Hurtado, and F. Janett. 2002. Comparison of Biociphos-plus and Tris-egg yolk extender for cryopreservatin of bull sperma. Theriogenology 57: 1087-1094.

Toelihere, M. R. 1993. Inseminasi Buatan pada Ternak. Penerbit Angkasa, Cetakan ke-3, Bandung.

Udin, Z., Hendri, dan Jaswandi. 2013. Optimalisasi Pemanfaatan Sumber Daya Lokal Sapi Pesisir Melalui Penerapan Teknik Kriopreservasi Sperma dan Manipulasi Reproduksi. Univ. Andalas. Padang. Hal. 15–45.

Umar, S. dan M. Maharani. 2005. Pengaruh berbagai waktu ekuilibrasi terhadap daya tahan sperma sapi Limousin dan uji kebuntingan. Jurnal Agribisnis Peternakan 1: 17−21.

DOI: https://doi.org/10.21059/buletinpeternak.v42i4.35173