Feed Evaluation Based on in Vitro Gas Production of Tropical Forages with Addition of Different Polyethylene Glycol (Peg) Level
Widya Kenshiana Putri(1*), Cuk Tri Noviandi(2), Kustantinah Adiwimarta(3)
(1) Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(2) Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(3) Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(*) Corresponding Author
Abstract
This study aimed to determine the chemical composition and gas production based on fermentation in the rumen of nine types of forage tropical feed commonly used in Indonesia. The forages used in this study were: mahogany leaves (Swietenia mahagoni L. Jacq.), tayuman leaves (Bauhinia purpurea), bamboo leaves (Bambusa arundinaceae), canary leaves (Canarium indicum L.), tea leaves (Camellia sinensis), ketapang leaves (Terminalia catapa L.), lamtoro leaves (Leucaena leucocephala), tehtehan leaves (Duranta repens), and turi leaves (Sesbania grandiflora). Measurements of in vitro gas production were carried out at 10 observation points (2, 4, 6, 8, 12, 16, 24, 36, 48, 72 hour). Tannin activity was measured for each sample at observation points using measurements of gas production divided into three groups with modified levels of polyethylene glycol (PEG), ie samples without PEG (P1); sample + PEG in the amount of 200 mgDM (P2); and PEG + samples of tannins contained in each forage based on literature studies (P3). Data were analyzed for a variance by following the factorial completely randomized design (CRD) pattern. Statistical analyzes were performed on all data by following the general linear procedure in PROC GLM from SAS. The data obtained were analyzed for variance at the 5% significance level. The results of gas production calculations showed that crude protein (CP) from each forage ranges from 5.75 - 22.37% where the highest CP was owned by turi leaves (S. grandiflora). The content of crude fiber (CF) ranged from 5.30 - 20.93%. The most optimal measurement of gas production was in the sample given PEG in the amount of 200mg/kg with a significant difference (P<0.05). The higher of the tannin content contained in the forage, the lower of gas produced. Measurement of tannin content showed that condensed tannin content varied from turi leaves by 0.20% to the highest in mahogany leaves by 8.60%. The addition of 200 mg/100mgDM of PEG optimizes the rate of forage gas production, especially for grass plants (gramineae).
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Abdulrazak S. A. 1995. The effects of supplementing roughage diets with leguminous tree forages on intake, digestion and performance of crossbred cattle in coastal lowland Kenya. PhD Thesis, University of Aberdeen, UK. 217 pp.
AOAC. 2005. Official Methods of Analysis of The Association of Official Analytical Chemists. Published by the Association of Official Analytical Chemists, Maryland, USA.
Barry, T. N., T. R. Manley, and S. J. Duncan. 1986. The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. Site of carbohydrate and protein digestion as influenced by dietary reactive tannin concentration. British Journal of Nutrition 55: 123–137.
Ben Salem, H., I. Ben Salem, and M. S. Ben-Said. 2005. Effect of the level and frequency of PEG supply on intake, digestion, biochemical and clinical parameters by goats given Kermes oak (QuercusCoccifera L.)-based diets. Small Rumin. Res. 56: 127–137.
Besharati, M. and A. Taghizadeh. 2011. Effect of Tannin-Binding Agents (Polyethylene Glycol and Polyvinylpyrrolidone) Supplementation on In Vitro Gas Production Kinetics of Some Grape Yield Byproducts. ISRN. Veterinary Science. 2011: 1 – 8. doi: 10.5402/2011/780540.
Brown, David, and J. W. Ng’ambi. 2017. Effect of polyethylene glycol 4000 supplementation on the performance of yearling male Pedi goats fed dietary mixture levels of Acacia karroo leaf meal and Setaria verticillata grass hay. Trop Anim. Health Prod. 49: 1051–1057.
Chen, X. 1994. Neway Program. International Feed Resources Unit. Rowett Research Institute. Backburn, Aberdeen.
Chen, Ji, S. K. Spear, J. G. Huddleston, and R. D. Rogers. 2005. Polyethylene glycol and solutions of polyethylene glycol as green reaction media. Green. Chem. 7: 64–82.
Christopher, D. L. 2012. Nutritionally related strategies for organic goat production. Small Rumin. Res. 39: 1–10.
Kamal, M. 1994. Nutrisi Ternak I. Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta.
Kustantinah. 2012. Pengukuran Kualitas Pakan Sapi. PT Citra Aji Parama, Yogyakarta.
Kustantinah, E. Indarto, Zuprizal, C. T. Noviandi, N. D. Dono, and F. A. Mukti. 2017. Feed Evaluation Based on Gas Production of Twelve Tropical Feedstuffs. The 7th International Seminar on Tropical Animal Production. Pp.178-184.
Le Bourvellec, C., S. Guyot, and C. M. Renard. 2004. Non-covalent interaction between procyanidins and apple cell wall material. Part I. Effect of some environmental parameters. Bio-chimica et Biophysica Acta 1672: 192–202.
Makkar, H. P. S. 2003. Effects and fate tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research. 49: 241-256.
McManus, J. P., K. G. Davis, J. E. Beart, S. H. Gaffney, T. H. Lilley, and E. Haslam. 1985. Polyphenol interactions. Part I. Introduction; some observations on the reversible complexation of Tannin and cell wall polyphenols with proteins and polysaccharides. Journal Chemical Society Perkin Trans II: 1429–1438.
Menke, K. H. L. Raab, A. SaleweCFi, H. Steingass, D. Fritz and W. Scheneider. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuff from the gas production when they are incubated with rumen liquor. J. Agric. Sci. 93: 217-222.
Reed, J. D. 1995. Nutritional toxicology of tannins and related polyphenols in forage legumes. Journal of Animal Science. 73: 1516-1528.
Silanikove, N., N. Gilboa, Z. Perevolotsky, and Z. Nitsan. 1996. Goats fed tannin-containing leaves do not exhibit toxic syndromes. Small Rumin. Res. 21: 195–201.
Yisehak, K., J. De-Boever, and G. P. J. Janssens. 2013. The effect of supplementing leaves of four tannin-rich plant species with polyethylene glycol on digestibility and zootechnical performance of zebu bulls (Bos indicus). Journal of Animal Physiology and Animal Nutrition 98: 431–438.
DOI: https://doi.org/10.21059/buletinpeternak.v45i1.58433
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