Association of Growth Hormone Gen with KUB Chicken Productivity

Prayoga Alfano(1), Depison Depison(2*), Silvia Erina(3)

(1) Animal Science Study Program, Faculty of Animal Science, University of Jambi, Jambi, 36361
(2) Animal Science Study Program, Faculty of Animal Science, University of Jambi, Jambi, 36361
(3) Animal Science Study Program, Faculty of Animal Science, University of Jambi, Jambi, 36361
(*) Corresponding Author


This study aims to investigate the diversity of quantitative characteristics and GH genes, along with the association between GH genes and quantitative characteristics. The research material consisted of 96 KUB chickens aged DOC-2 months and corresponding blood samples. The t-test was utilized to determine differences in body weight, weight gain, and body measurements between male and female KUB chickens, and assess the diversity of the GH gene. T2-Hotelling analysis was employed to compare body measurements between male and female KUB chickens, while principal component analysis was used to identify size and shape characteristics. Male KUB chickens' average body weight, weight gain and body size were significantly (p<0.05) higher than females. The analysis of the growth hormone gene exhibited three genotypes: +/+ (0.51), +/- (0.35), and -/- (0.14). Additionally, two alleles were identified: (+) accounting for 0.68% and (-) accounting for 0.32%. The growth hormone gene MspI of KUB chickens demonstrated polymorphism, with X2 count (2.93) < X2 table 0.05 (3.84). The heterozygosity value in the KUB chicken population was 0.43, and the obtained PIC value was 0.38. The genotype +/+ of the growth hormone gene in KUB chickens was significantly higher (p<0.05) than genotypes +/- and -/-.In conclusion, male KUB chickens exhibited higher body weight, weight gain, and body measurements compared to female KUB chickens. The chest circumference served as the identifier for body size in both male and female KUB chickens, while the length of the upper body and tibia length distinguished the shape characteristics of male and female KUB chickens, respectively. KUB chickens' growth hormone gene MspI was associated with body weight, weight gain, and body measurements, with the (+/+) genotype being the most favorable


Characterization; association; growth hormone (GH) gene; KUB chicken; MspI enzyme.

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Pagala. 2020. Identification of TGF-β2 general diversity and Its association with growth properties in Tolaki chicken. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan 8: 22-29.

Ananda, M. K., P. Sampurna, and T. S. Nindhia. 2020. Estimation of carcass weight of male and female Bali cattle based on body length and chest circumference. Indonesia Medicus Veterinus 9: 512–521.

Andini, A. S. and D. Purwantini. 2019. Identification of quantitative characteristic and association between ACTA-1 gene and body weight in Local chicken. In IOP Conference Series: Earth and Environmental Science 1: 372-375.

Anggraini, A. D., E. B. Khoendori, H. Pramono and D. J. Wahyono. 2017. Polymorphism analysis of the coagulase gene in isolates of methicillinresistant Staphylococcus aureus with aluI restriction site. Health Science Journal of Indonesia 8: 19-24.

Arianto, H., I. Wahyuni, and E. Kurnianto. 2019. Analysis of growth parameters of second generation Red comb and Black comb Kedu chicken in Balai Pembibitan dan Budidaya Ternak non ruminansia satker ayam Maron Kabupaten Temanggung. Jurnal Peternakan Indonesia 21: 10-17.

Azizah, S. N., A, Nuryanto and H. Pramono. 2015. Karakteristik molekuler ikan gurami soang (Osphronemus goramy Lac.) berbeda ukuran menggunakan PCR-RFLP gen Sitoktrom C oksidasi 1. Jurnal Biosfera 32: 185-193.

Batubara, A., S. Elieser, and C. Sumantri. 2016. Study of BMP15 gene polymorphism in Boer, Kacang, and Boerka goats. Jurnal Ilmu Ternak dan Veteriner 21: 224-230.

Botstein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32: 314–331.

Depison, Gushairyanto and D. Irmaya. 2022. Characterization phenotype and genetic distance some of the native chicken strains in jambi province. Iraqi Journal of Agricultural Sciences. 53: 1154-1166

Gaspersz, V. 2006. Teknik Analisis dalam Penelitian Percobaan. Penerbit Tarsito, Bandung.

Gurning, S. D. S., I. N. Wandia and I. G. Soma. 2018. Characteristic of the D8S1100 microsatellite Locus in the population of long tailed Macaca at mount pengsong Lombok. Jurnal Indonesia Medicus Vterinus 7: 540-550

Hidayati, E. Saleh, and T. Aulawi. 2016. Identification of the genetic diversity of BMPR-1B (bone morphogenetic protein receptor IB) in Arabic chicken, Kampung chicken, and laying hens using PCR-RFLP. Anim. J. 13: 1–12.

Hutami, R., H. Bisyri, Sukarno, H. Nuraini, and R. Ranasasmita. 2018. DNA Extraction from Raw meat for analysis with the Loop-Mediated Isothermal Amplification (LAMP) Method. Jurnal Agroindustri Halal 4: 209-216.

Irmaya, D., Depison and Gurhairiyanto. 2021. Quantitative characteristic of indonesian native chickens at the age of 4 months. Livestock and Animal Research 19: 108-119.

Mardiah, T., Depison, and E. Adiyanto. 2021. Phenotype diversity and gene myostatin (MSTN) of Bangkok chicken using PCR-RFLP. Buletin Peternakan 45: 233-240

Mayora, W. I., S. Tantalo, K. Nova, and R. Sutrisna. 2018. Performance of the starter on KUB chicken rationing with different crude protein. Jurnal Riset dan Inovasi Peternakan 2: 26-31.

Mazurowski, A., A. Frieske, D. Kokoszyński, S. Mroczkowski, Z. Bernacki, and A. Wilkanowska. 2015. Examination of growth hormone (GH) gene polymorphism and its association with body weight and selected body dimensions in ducks. Folia Biologica (Kraków) 63: 43-50.

Miraj, N. N., C. Sumantri, S. Murtini, and N. Ulupi. 2022. Polymorphisms of the BG1 gene as a candidate for disease resistance characterizing genes in IPB-D2 prospective chicken lines. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan 10: 144-151.

Muzani, A. B., C. Brahmantiyo, Sumantri, and A. Tapyadi. 2005. Estimation of genetic distance in Cihateup, Cirebon and Mojosari ducks. Livestock Media. 28: 109-116.

Nei, M. and S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford University Press. New York.

Pagala, M. A., A. Selamet, B. Rusli, and H. Has. 2018. Phenotypic and genotypic characteristics of the GH (growth hormone) gene in Tolaki chickens. Journal of Tropical Animal Husbandry Science and Technology 5: 1-4.

Prawira, R., Depison, Gushariyanto, and S. Erina. 2021. The relationship between egg morphology with egg weight and DOC weight with body weight of Kampung F1. Jurnal Ilmu Peternakan Terapan 5: 19-30.

Putra, A., Rukmiasih, and R. Afnan. 2015. Percentage and carcass quality of Cihateup-Alabio (CA) ducks at different slaughter ages. Department of Animal Production Science and Technology 3: 27- 32.

Puteri, G. A., B. Utomo, and R. Darsono. 2019. Growth hormone (Gh) gene profile of Madura cattle cross breeding Limousin cattle With PCR RFLP Method. Ovozoa 8: 43-48.

Putri, A. B. S. R. N., Gushairiyanto, and Depison. 2020. Body Weight and Morphometric Characteristics of Some Local Chicken Strain. Journal of Tropical Animal Scince and Technology (JITRO) 7: 256-263.

Rahmadhan, D., R. Sari, and P. Apridamayanti. 2019. Pengaruh suhu annealing terhadap amplifikasi gen tem menggunakan primer dengan %gc rendah. Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN 4: 1-7

Sari, M., Depison, Gushariyanto, and E. Wiyanto. 2021. The Relationship Between Egg Weight with Hatching Weight and Hatching Weight with Body Weight G1 of Merawang Chicken Aged up to 4 Months. Jurnal Peternakan 18: 147-159.

Sihombing, T. I. E., N. Wandia and I. G. Soma. 2019. Polymorphism of microsatellite D2S1368 locus in long tail Monkey population in puncak Mundi Temple, Nusa Penida island, Klungkung, Bali. Jurnal Indonesia Medicus Veterinus 8: 806-814.

Terryana, B. T., K. Nugroho, Reflinur, K. Mulya, N. Dewi and P. Lestari. 2017. Genotypic and phenotypic diversities of 48 Introduced Soybean accessions originated from China. Jurnal Agro Biogen 13: 1-16.

Urfa, S., H. Indijani, and W. Tanwiriah. 2017. Growth curve model of Kampung Unggul Balitnak (KUB) Chicken. Jurnal Ilmu Ternak 17: 59–66.

Utama, M., Depison, Gushairiyanto, and H. Ediyanto. 2022. Comparison of germination, hatchability, and quantitative characteristics of KUB chickens and Kampung chickens (G1). Journal of Tropical Animal Science and Technology 9: 95-103.

Wang, J. L. Raskin, D. C. Samuels, Y. Shyr, and Y. Guo. 2015. Genome measures used for quality control are dependent on gene function and ancestry. Bioinformatics 31: 318–323.

Zurriyati, Y., D. Sisriyenni, N. E. Deni, and Dahono. 2020. Productivity performance of balitbangtan superior local chicken in Kampar District Riau Province. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner 3: 502-511.


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