Different Flushing Frequency on Blood Metabolites Profile of Ewes and Their Lambs at Pre-Weaning Period

https://doi.org/10.21059/buletinpeternak.v48i3.93637

Dilla Mareistia Fassah(1), Mila Taniasari(2), Fanny Rahmasari Daeli(3), Didid Diapari(4), Dewi Apri Astuti(5), Lilis Khotijah(6*)

(1) Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680
(2) Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680
(3) Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680
(4) Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680
(5) Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680
(6) Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680
(*) Corresponding Author

Abstract


This study determined the effects of different flushing frequency on performance and blood metabolite profile of ewes and their suckling lambs at pre-weaning period. Twelve multiparous Garut ewes (2 years-old, BW 30.06 ± 6.20 kg) and 18 lambs born to experimental ewes (lambing weight 2.49 ± 0.56 kg) were used in this study. Ewes were randomly assigned into four treatment groups in a complete block design, namely: without flushing (T0: control), flushing at the beginning of mating (T1: 2 weeks before and after mating), two times flushing (T2: T1 + 4 weeks flushing at mid-gestation), and three times flushing (T3: T2 + 2 weeks flushing at before and after parturition). Three times flushing increased ((p<0.05) the crude fat intake, while different flushing frequencies did not affect (p>0.05) dry matter intake and intakes of crude protein, crude fiber, nitrogen-free extract, and total digestible nutrients of ewes at the pre-weaning period. Different flushing frequencies did not change (p>0.05) the productive performances of ewes during the pre-weaning period. Flushing application improved (p<0.05) the average daily gain of pre-weaning lambs at 14 days, but it did not affect the weaning weight of lambs. Two times flushing showed no pre-weaning mortality rate (p<0.05). Flushing application tended to decrease (p=0.08) blood plasma triglyceride of ewes at 21- days, while two times flushing frequency tended to increase blood plasma cholesterol (p=0.05) and triglyceride (p=0.08) of lambs at 21 days. In conclusion, increased flushing frequency supports ewes and their twin lamb growth performance and blood metabolite profile at the pre-weaning period

Keywords


Blood metabolites, Flushing frequency, Growth, Performance, Pre-weaning

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References

Abecia, J. A., S. Luis, and F. Canto. 2021. Implanting melatonin at lambing enhances lamb growth and maintains high fat content in milk. Veterinary Research Communications 45: 181–188. https://doi.org/10.1007/s11259-021-09799-y

Abuzahra, M., D. Wijayanti, M. H. Effendi, I. Mustofa, and L. A. Eid. 2024. Estimate the effect of non-genetic factors on the reproductive traits of Afec-Assaf strain in Bani Naim Farm, Palestine. Jurnal Ilmu Ternak dan Veteriner 29: 9–15. https://doi.org/10.14334/jitv.v29i1.3213

Ahmed, S. 2017. Pre and post-natal nutrition of ewes on the performances of native Bengal Ewes and their lambs. Animal and Veterinary Sciences 5: 33. https://doi.org/10.11648/j.avs.20170502.12

Antunović, Z., M. Šperanda, J. Novoselec, M., Didara, B. Mioč, Ž. Klir, and D. Samac. 2017. Blood metabolic profile and acid-base balance of dairy goats and their kids during lactation. Croatia Veterinarski Arhiv 87: 43–55.

AOAC. 2019. Official Methods of Analysis of AOAC International 21st edn. G. W. Latimer (ed). AOAC International, Washington DC.

Astuti, A., R. Rochijan, B. P. Widyobroto, and C. T. Noviandi. 2022. Nutrient status, hematological and blood metabolite profile of mid-lactating dairy cows during wet and dry seasons raised under Indonesian tropical environmental conditions. Journal of Animal Behaviour and Biometeorology 10: 1–6. https://doi.org/10.31893/ jabb.22007

Averós, X., I. Granado-Tajada, J. Arranz, I. Beltrán de Heredia, L. González, R. Ruiz, A. García-Rodríguez, and R. Atxaerandio. 2022. Pre-partum supplementation with polyunsaturated fatty acids on colostrum characteristics and lamb immunity and behavior after a mild post-weaning aversive handling period. Animals 12: https://doi.org/10.3390/ani12141780

Behrendt, R., J. E. Hocking Edwards, D. Gordon, M. Hyder, M. Kelly, F. Cameron, J. Byron, M. Raeside, G. Kearney, and A. N. Thompson. 2019. Offering maternal composite ewes higher levels of nutrition from mid-pregnancy to lambing results in predictable increases in birthweight, survival, and weaning weight of their lambs. Animal Production Science 59: 1906–1922. https://doi.org/10.1071/AN18505

Bianchi, A. E., T. Zortea, C. J. Cazzarotto, G. Machado, L. G. Pellegrini, N. S. P. Dos Santos Richards, M. D. Baldissera, A. S. Da Silva, A. C. Galvão, and V. D. P. Macedo. 2018. Addition of palm oil in diet of dairy ewes reduces saturated fatty acid and increases unsaturated fatty acids in milk. Acta Scientiae Veterinariae 46: 10. https://doi.org/10.22456/1679-9216.89180

Chay-Canul, A. J., E. Aguilar-Urquizo, G. M. Parra-Bracamonte, Á. T. Piñeiro-Vazquez, J. R. Sanginés-García, J. G. Magaña-Monforte, R. A. García-Herrera, and N. López-Villalobos. 2019. Ewe and lamb pre-weaning performance of Pelibuey and Katahdin hair sheep breeds under humid tropical conditions. Italian Journal of Animal Science 18: 850–857. https://doi.org/10. 1080/1828051X.2019.1599305

Coleman, D. N., K. D. Murphy, and A. E. Relling. 2018. Prepartum fatty acid supplementation in sheep. II. Supplementation of eicosapentaenoic acid and docosahexaenoic acid during late gestation alters the fatty acid profile of plasma, colostrum, milk and adipose tissue, and increases lipogenic gene expression of adipose tissue1. Journal of Animal Science 96: 1181–1204. https://doi.org/10.1093/ jas/skx013

Conte, G., T. Giordani, A. Vangelisti, A. Serra, M. Pauselli, A. Cavallini, and M. Mele. 2021. Transcriptome adaptation of the ovine mammary gland to dietary supplementation of extruded linseed. Animals 11: 2707. https://doi.org/10.3390/ani11092707

Cordero, M. O., C. A. M. Herrera, J. M. V. García, C. A. Stewart, C. A. R. Nieto, A. E. O. Alfaro, I. W. Purvis, V. C. Reyes, H. A. L. Rangel, and G. B. Martin. 2019. Pregnancy and litter size, but not lamb sex, affect feed intake and wool production by merino-type ewes. Animals 9: 214. https://doi.org/10. 3390/ani9050214

Countouris, M. E., C. Holzman, A. D. Althouse, G. G. Snyder, E. Barinas-Mitchell, S. E. Reis, and J. M. Catov. 2020. Lactation and maternal subclinical atherosclerosis among women with and without a history of hypertensive disorders of pregnancy. Journal of Women’s Health 29: 789–798. https://doi.org/10.1089/jwh.2019.7863

Dari D. W., M. Astawan, N. Wulandari, and H. Suseno. 2017. Karakteristik minyak ikan sardin (Sardinella sp.) Hasil pemurnian bertingkat. Jurnal Pengolahan Hasil Perikanan Indonesia 20: 455–467.

Douh, M., A. Hicher, A. Saddek, and C. Aissaoui. 2019. Lambing and mortality rate in Ouled-Djellal sheeps in Tebessa region-Algeria. Iraqi Journal of Veterinary Sciences 32: 267–273. https://doi.org/10.33899/ijvs.2019.153863

Dove, H., M. Freer, and J. Z. Foot. 2018. The nutrition of grazing ewes during pregnancy and lactation: Relationships between herbage, supplement and milk intakes, and ewe and lamb liveweight and body composition. Animal Production Science 58: 1253–1270. https://doi.org/10. 1071/AN16541

Farrag, B. 2019. Productive characteristics and reproductive responses to estrus synchronization and flushing in abou-delik ewes grazing in arid rangelands in halaieb - shalateen - abouramad triangle of Egypt. Journal of World’s Poultry Research 9: 201–210. https://doi.org/10.36380/scil. 2019.wvj26

Farrag, B., K. A. El-Bahrawy, H. A. Shedeed, and M. A.-H. El-Rayes. 2024. Effect of protected fatty acid supplementation on ovarian activity, reproductive hormone profiles and reproduction of Barki ewes under semiarid conditions. Archives Animal Breeding 67: 111–122. https://doi.org/10.5194/aab-67-111-2024

Flinn, T., D. O. Kleemann, A. M. Swinbourne, J. M. Kelly, A. C. Weaver, S. K. Walker, K. L. Gatford, K. L. Kind, and W. H. E. J. van Wettere. 2020. Neonatal lamb mortality: major risk factors and the potential ameliorative role of melatonin. Journal of Animal Science and Biotechnology 11: 107. https://doi.org/10.1186/s40104-020-00510-w

Getahun, D., T. Alemneh, D. Akeberegn, M. Getabalew, and D. Zewdie. 2019. Urea metabolism and recycling in ruminants. Biomedical Journal of Scientific & Technical Research 20: 14790-14796. https://doi.org/10.26717/BJSTR.2019.20.003401

Ghaffari, M. H., J. A. R. MacPherson, H. Berends, and M. A. Steele. 2017. Diurnal variation of NMR based blood metabolites in calves fed a high plane of milk replacer: a pilot study. BMC Veterinary Research 13: 271. https://doi.org/10.1186/s12917-017-1185-2

Goldansaz, S. A., A. C. Guo, T. Sajed, M. A. Steele, G. S. Plastow, and D. S. Wishart. 2017. Livestock metabolomics and the livestock metabolome: A systematic review. PLoS ONE 12: e0177675. https://doi.org/10. 1371/journal.pone.0177675

Harjanti, W. A., D. W. Harjanti, P. Sambodho, and S. A. B. Santoso. 2017. Pengaruh suplementasi baking soda dalam pakan terhadap urea darah dan urea susu sapi perah laktasi. Jurnal Peternakan Indonesia 19: 65. https://doi.org/10.25077/jpi.19.2.65-71.2017

Hernández, J., J. L. Benedito, and C. Castillo. 2020. Relevance of the study of metabolic profiles in sheep and goat flock. Present and future: A review. Spanish Journal of Agricultural Research 18: 1–14. https://doi.org/10.5424/sjar/2020183-14627

Huang, G., J. Wang, K. Liu, F. Wang, N. Zheng, S. Zhao, X. Qu, J. Yu, Y. Zhang, and J. Wang. 2022. Effect of flaxseed supplementation on milk and plasma fatty acid composition and plasma parameters of Holstein Dairy cows. Animals 12: 1898. https://doi.org/10. 3390/ani12151898

Kaneko, J., J. Harvey, and M. L. Bruss. 2008. Clinical Biochemistry of Domestic Animals 6th edn. Academic Press, Burlington.

Kenyon, P. R., F. J. Roca Fraga, S. Blumer, and A. N. Thompson. 2019. Triplet lambs and their dams – a review of current knowledge and management systems. New Zealand Journal of Agricultural Research 62: 399–437. https://doi.org/10.1080/00288233. 2019.1616568

Khayat, S., H. Fanaei, and A. Ghanbarzehi. 2017. Minerals in pregnancy and lactation: A review article. Journal of Clinical and Diagnostic Research 11: QE01–QE05. https://doi.org/10.7860/JCDR/2017/28485.10626

Khotijah, L., N. Arofah, K. Erlangga, S. H. Wijaya, D. Diapari, K. Komalasari, and D. A. Astuti. 2022. Reproductive performance of ewes, fed flushing diet at different management feeding program. Pakistan Journal of Biological Science 25: 827-834. https://doi.org/10.3923/pjbs.2022.827.834.

Kosasih, W., R. T. Rosmalina, C. Risdian, S. Priatni, W. Kosasih, and E. Saepudin. 2021. Production of omega-3 fatty acids by enzymatic hydrolysis from lemuru fish by-products. Sains Malaysiana 50: 2271–2282. https://doi.org/10.17576/jsm-2021-5008-11

Martins, A. S., D. M. Polizel, G. B. Oliveira, J. P. R. Barroso, M. V. de C. Ferraz Jr., A. A. Miszura, M. Baggio, E. M. Ferreira, and A. V. Pires. 2022. Narasin improves ewe milk yield efficiency and may affect performance of lambs. Scientia Agricola 79: 1-8. https://doi.org/10.1590/1678-992x-2020-0334

Mostafa, A. and M. Farghal. 2022. Effect of flushing with energy or protein sources on the reproductive performance in Ossimi ewes. Journal of Veterinary Medical Research 29: 38-42. https://doi.org/10.21608/jvmr. 2022.133773.1053

Nguyen, Q., H. Le, D. Nguyen, P. Nish, J. Otto, B. Malau-Aduli, P. Nichols, and A. E. O. Malau-Aduli. 2018. Supplementing dairy ewes grazing low quality pastures with plant-derived and rumen-protected oils containing eicosapentaenoic acid and docosahexaenoic acid pellets increases body condition score and milk, fat, and protein yields. Animals 8: 241. https://doi.org/10.3390/ani8120241

Nguyen, Q. V., H. Van Le, D. V. Nguyen, B. S. Malau-Aduli, P. D. Nichols, and A. E. O. Malau-Aduli. 2019. Enhancement of dairy sheep cheese eating quality with increased n-3 long-chain polyunsaturated fatty acids. Journal of Dairy Science 102: 211–222. https://doi.org/10.3168/jds.2018-15215

Nickles, K. R., L. Hamer, D. N. Coleman, and A. E. Relling. 2019. Supplementation with eicosapentaenoic and docosahexaenoic acids in late gestation in ewes changes adipose tissue gene expression in the ewe and growth and plasma concentration of ghrelin in the offspring. J. Anim. Sci. 97: 2631–2643. https://doi.org/10.1093/jas/ skz141

Novilla, A., P. Nursidika, and W. Mahargyani. 2017. Komposisi asam lemak minyak kelapa murni (virgin coconut oil) yang berpotensi sebagai anti kandidiasis. Educhemia 2: 161-173. https://doi.org/10.30870/ educhemia.v2i2.1447

Nugroho, P., K. G. Wiryawan, W. Manalu, and D. A. Astuti. 2020. Blood hematology profile at postpartum in Ettawa grade does fed with different fatty acid flushing diets during the late gestation period and different litter sizes. E3S Web of Conferences 151: 01002. https://doi.org/10.1051/e3sconf/ 202015101002

Nurlatifah, A., L. Khotijah, R. I. Arifiantini, M. S. Maidin, and D. A. Astuti. 2022. Colostrum quality of ewe fed flushing diet containing epa and dha associated with lamb performance. Tropical Animal Science Journal 45: 348–355. https://doi.org/10. 5398/tasj.2022.45.3.348

Nurlatifah, A., L. Khotijah, R. I. Arifiantini, M. S. Maidin, D. A. Astuti, and H. Herdis. 2023. Immunity and behaviour of lambs born from ewes fed a flushing diet containing EPA and DHA. Jurnal Ilmu Ternak dan Veteriner 28: 159–168. https://doi.org/10.14334/jitv. v28i3.3110

Nurlatifah, A., L. Khotijah, K. Komalasari, and D. A. Astuti. 2020. The effect of flushing with fatty acid supplementation in ewes ration on folliculogenesis. IOP Conference Series: Earth and Environmental Science 411: 012036. https://doi.org/10.1088/1755-1315/411/1/012036

Opsomer, G., M. Van Eetvelde, M. Kamal, and A. Van Soom. 2017. Epidemiological evidence for metabolic programming in dairy cattle. Reproduction, Fertility and Development, 29: 52–57. https://doi.org/10.1071/ RD16410

Oscarsson, J. and E. Hurt-Camejo. 2017. Omega-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid and their mechanisms of action on apolipoprotein B-containing lipoproteins in humans: a review. Lipids in Health and Disease 16: 149. https://doi.org/10.1186/s12944-017-0541-3

Parente, M. O. M., I. Susin, C. P. Nolli, E. M. Ferreira, R. S. Gentil, D. M. Polizel, A. V. Pires, S. P. Alves, and R. J. B. Bessa. 2018. Effects of supplementation with vegetable oils, including castor oil, on milk production of ewes and on growth of their lambs. J. Anim. Sci. 96: 354-363. https://doi.org/10.1093/jas/skx015

Peng, Z., C. Zhang, L. Yan, Y. Zhang, Z. Yang, J. Wang, and C. Song. 2020. EPA is more effective than DHA to improve depression-like behavior, glia cell dysfunction and hippcampal apoptosis signaling in a chronic stress-induced rat model of depression. Int. J. Mol. Sci. 21: 1769. https://doi.org/10.3390/ijms21051769

Pesántez-Pacheco, J. L., A. Heras-Molina, L. Torres-Rovira, M. V. Sanz-Fernández, C. García-Contreras, M. Vázquez-Gómez, P. Feyjoo, E. Cáceres, M. Frías-Mateo, F. Hernández, P. Martínez-Ros, J. V. González-Martin, A. González-Bulnes, and S. Astiz. 2019a. Influence of maternal factors (Weight, body condition, parity, and pregnancy rank) on plasma metabolites of dairy ewes and their lambs. Animals 9: 1–19. https://doi.org/10.3390/ani9040122

Pesántez-Pacheco, J. L., A. Heras-Molina, L. Torres-Rovira, M. V. Sanz-Fernández, C. García-Contreras, M. Vázquez-Gómez, P. Feyjoo, E. Cáceres, M. Frías-Mateo, F. Hernández, P. Martínez-Ros, J. V. González-Martin, A. González-Bulnes, and S. Astiz. 2019b. Maternal metabolic demands caused by pregnancy and lactation: Association with productivity and offspring phenotype in high-yielding dairy ewes. Animals 9: https://doi.org/10. 3390/ani9060295

Rathod, P., S. S. Chaudhary, V. K. Singh, T. D. Manat, A. B. Joshi, and B. S. Siddhapara. 2024. Biochemical analytes during different stages of lactation in Surti buffaloes. Int. J. Adv. Biochem. Res. 8: 330–333. https://doi.org/10.33545/26174693.2024.v8.i5Se.1183

Ridler, A. L., K. J. Flay, P. R. Kenyon, H. T. Blair, R. A. Corner-Thomas, and E. J. Pettigrew. 2022. Factors associated with mortality of lambs born to ewe hoggets. Animals 12: https://doi.org/10.3390/ani12030319

Rizzo, F. A., A. C. Fluck, J. Schafhäuser Junior, R. B. Scheibler, J. L. Nörnberg, D. P. deVargas, L. A. Lourenço, A. P. B. de Souza, and J. L. S. da Silva. 2024. Sunflower oil supplementation in the diets of lactating cows: productive and nutritional performance. Semina: Ciências Agrárias 44: 329–344. https://doi.org/10.5433/1679-0359.2024v44n2p329

Roche, J. R., C. R. Burke, M. A. Crookenden, A. Heiser, J. L. Loor, S. Meier, M. D. Mitchell, C. V. C. Phyn, and S. A. Turner. 2018. Fertility and the transition dairy cow. Reproduction, Fertility and Development 30: 85–100. https://doi.org/10.1071/ RD17412

Rosales Nieto, C. A., M. B. Ferguson, C. A. Macleay, J. R. Briegel, D. A. Wood, G. B. Martin, R. Bencini, and A. N. Thompson. 2018. Milk production and composition, and progeny performance in young ewes with high merit for rapid growth and muscle and fat accumulation. Animal 12: 2292–2299. https://doi.org/10.1017/S1751731118000307

Sarvinda, T. D., S. Bintara, I. G. P. Budisatria, Kustantinah, and E. Baliarti. 2022. The effect of flushing premating with spirulina platensis supplementation on ewes postpartum estrus. Buletin Peternakan 46: 31-35. https://doi.org/10.21059/buletin peternak.v46i1.67523

Silva, J. V. V., S. Ganesan, H. K. J. P. Wickramasinghe, N. Stepanchenko, C. A. Kaya, D. C. Beitz, and J. A. D. R. N. Appuhamy. 2022. Effects of branched-chain amino acids on glucose uptake and lactose synthesis rates in bovine mammary epithelial cells and lactating mammary tissue slices. Journal of Dairy Science 105: 1717–1730. https://doi.org/10.3168/jds. 2021-20950

Sudarman, A., H. Fatmiati, and L. Khotijah. 2019. Formulasi susu pengganti dan evaluasi pengaruhnya terhadap performa anak domba kembar. Jurnal Sain Peternakan Indonesia 14: 228–236. https://doi.org/ 10.31186/jspi.id.14.3.228-236

Tharwat, L., A.-E. R. A.-E. M. Abd-El Raheem, and A. E.-S. A. Mohamed. 2023. Effect of transition period in buffalo cows on some biochemical parameters. SVU-International Journal of Veterinary Sciences 6: 46–62. https://doi.org/10.21608/svu.2023.170430.1234

Varanis, L. F. M., E. B. Schultz, K. A. Oliveira, L. F. Sousa, W. F. G. da Cruz, and G. de-L. M. Júnior. 2021. Serum biochemical reference ranges for lambs from birth to 1 year of age in the tropics. Semina: Ciéncias Agrárias 42: 1725-1740. https://doi.org/10.14393/BJ-v37n0a2021-47695

Varanis, L. F. M., K. A, Oliveira, C. M. Araújo, W. F. G. da Cruz, and G. de-L. M. Júnior. 2021b. Serum biochemical reference ranges for pregnant sheep. Bioscience Journal 37: e37036. https://doi.org/10. 14393/BJ-v37n0a2021-47695

Wang, X., Y. Wang, Q. Wang, C. Dai, J. Li, P. Huang, Y. Li, X. Ding, J. Huang, T. Hussain, and H. Yang. 2023. Effect of dietary protein on growth performance, and serum biochemical index in late pregnant Hu ewes and their offspring. Animal Biotechnology 34: 97–105. https://doi.org/10.1080/ 10495398.2021.1939042

Wardeh, M. F. 1981. Models for estimating energy and protein utilization for feeds [Dissertation, Utah State University]. https://doi.org/https://doi.org/10.26076/9026-5aad. Accessed 20 September 2020.

Whatford, L., B. D. Chivers, M. Rowe, and N. Blackie. 2023. A survey of the current farming practices and perceptions on adopting orphan lambs in the United Kingdom: How do “ewe” do it?. Ruminants 3: 468–482. https://doi.org/10.3390/ ruminants3040038

Zdorovieva, E., G. Boryaev, E. Kistanova, A. Nosov, Y. Fedorov, and N. Semigodov. 2019. Protected fat in the diet of lactating ewes affects milk composition, lamb body weight and their biochemical parameters. Bulgarian Journal of Agricultural Science 25: 1277-1280.



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