Taguchi-Assisted of Ligninase Production by  Phanerochaete chrysosporium  ITB Isolate Biofilm

Siti Mutmainah; Roswanira Abdul Wahab; Eli Hendrik Sanjaya; Evi Susanti

doi:10.22146/ijc.103401

Taguchi-Assisted of Ligninase Production by Phanerochaete chrysosporium ITB Isolate Biofilm

https://doi.org/10.22146/ijc.103401

Siti Mutmainah⁽¹⁾, Roswanira Abdul Wahab⁽²⁾, Eli Hendrik Sanjaya⁽³⁾, Evi Susanti^(4*)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang 65145, Indonesia
(2) Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang 65145, Indonesia; Biotechnology Study Program, Department of Applied Science, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang 65145, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang 65145, Indonesia; Biotechnology Study Program, Department of Applied Science, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang 65145, Indonesia
(*) Corresponding Author

Abstract

The Phanerochaete chrysosporium ITB isolate can form a biofilm when grown on a support material, in which the attached cells are in a sessile state, which allows the cells to thrive better compared to other conditions. This study uses the Taguchi model to explore the ligninase (LiP, MnP, and laccase) production by P. chrysosporium ITB isolates immobilized on polypropylene (PP). This research was carried out in a laboratory experimental manner, which consisted of stages that included the preparation of spore suspension and optimization of conditions for the production of ligninase using Taguchi. The results showed that lignin peroxidase (LiP) and laccase (420.1 and 78.69 U/mg) enzymes were optimally produced using an inoculum size of 5 × 10³ spore/mL, with a growth duration of 6 d at pH 3 and required 0.4 g/mL of PP to yield a 0.36% biofilm weight. The optimal production of MnP (410.23 U/mg) warranted an inoculum size of 5 × 10² spore/mL with incubation of 4 d at pH 5, and immobilization on 0.20 g/mL PP, to give a final biofilm weight of 0.09%. These results indicate a potential LiP and laccase production by the biofilm of P. chrysosporium on the polypropylene as supporting materials.

Keywords

biofilm; P. chrysosporium ITB isolate; ligninase; enzyme specific activity; Taguchi model

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DOI: https://doi.org/10.22146/ijc.103401