Simultaneous HPLC Analysis of Asiaticoside and α-Mangostin in Nanoethosomal Carriers

Pedro Anugerah Aswan; Baitha Palanggatan Maggadani; Wilzar Fachri; Raditya Iswandana

doi:10.22146/ijc.111150

Simultaneous HPLC Analysis of Asiaticoside and α-Mangostin in Nanoethosomal Carriers

https://doi.org/10.22146/ijc.111150

Pedro Anugerah Aswan⁽¹⁾, Baitha Palanggatan Maggadani⁽²⁾, Wilzar Fachri⁽³⁾, Raditya Iswandana^(4*)

(1) Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok 16424, Indonesia
(2) Laboratory of Pharmaceutical-Medicinal Chemistry and Bioanalysis, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok 16424, Indonesia; Bioanalysis in Biological Matrices Research Cluster, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok 16424, Indonesia
(3) Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6, Jakarta 10430, Indonesia
(4) Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok 16424, Indonesia; Drug Delivery System Research Cluster, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok 16424, Indonesia
(*) Corresponding Author

Abstract

The development of robust analytical methods is essential for quality control of nanoethosomal formulations containing asiaticoside and α-mangostin, bioactive compounds with anti-inflammatory and antioxidant properties. Simultaneous quantification within nanocarriers is challenging and requires validated techniques. This study established a precise HPLC method, in accordance with ICH Q2(R2) guidelines, for the concurrent determination of both compounds. The technique demonstrated excellent linearity (R² > 0.999), precision (%RSD < 2%), and accuracy (95–105% recovery). The nanoethosomal system also achieved high encapsulation efficiencies of 94.76% for asiaticoside and 99.27% for α-mangostin. This validated method provides a reliable tool for standardizing these complex nano-formulations, supporting their further development and clinical translation.

Keywords

HPLC; validation; asiaticoside; α-mangostin; nanoethosome

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DOI: https://doi.org/10.22146/ijc.111150