Analytical Method Development for the Determination of Α-Endosulfan and Bifenthrin Pesticide Residues in Tea

https://doi.org/10.22146/ijc.21262

Dyah Styarini(1*), Andreas Andreas(2), Y. Susanto Ridwan(3), Retno Yusiasih(4)

(1) Division of Analytical Chemistry and Standard, Research Center for Chemistry, Indonesian Institute of Sciences, Komplek Puspiptek, Serpong, Tangerang, Banten 15314
(2) Division of Analytical Chemistry and Standard, Research Center for Chemistry, Indonesian Institute of Sciences, Jl. Cisitu-Sangkuriang, Bandung 40135
(3) Division of Analytical Chemistry and Standard, Research Center for Chemistry, Indonesian Institute of Sciences, Jl. Cisitu-Sangkuriang, Bandung 40135
(4) Division of Analytical Chemistry and Standard, Research Center for Chemistry, Indonesian Institute of Sciences, Jl. Cisitu-Sangkuriang, Bandung 40135
(*) Corresponding Author

Abstract


The development of analytical method for the determination of α-endosulfan and bifenthrin residues in tea has been done. The complex matrices and also the pigment were the challenge in doing quantification of the pesticide residues in tea matrices. In order to get appropriate analysis method for the determination of pesticide residues in tea, the modification was done in the analytical method for the determination of organochlorine multiresidue in non fat matrices: seasoning and spicy that is published by Directorate General of Food Crops, Directorate of Food Plant Protection. The modification was done particularly in clean-up step to remove the interferences from the extract of tea matrices such as the pigment that usually interfere the measurement with Gas Chromatography (GC). The result showed that the MDL value for both analytes were 0.5 ng/g that were much lower than MRLs. The percent recovery obtained from the method was 78.58 and 90.19% for α-endosulfan and bifenthrin, respectively. The precision of the analysis method for both analytes were good since the % RSD values were below than the Horwitz’s value that was 19.18% at spiking level concentration of 300 ng/g.

Keywords


α-endosulfan; bifenthrin; GC-ECD; tea; pesticide

Full Text:

Full Text PDF


References

[1] Dini Jamia Rayati, 2008, Warta Pusat Penelitian Teh dan Kina, 19 (1-3), 27–56.

[2] Peraturan Pemerintah RI No. 6 Tahun 1995 tentang Perlindungan tanaman dan keputusan bersama menteri kesehatan dan menteri pertanian tentang Batas maksimum Residu Pestisida pada hasil Pertanian.

[3] Aybar-Muñoz, J., Fernández-González, E., García-Ayuso, L.E., González-Casado, A., and Cuadros-Rodríguez, L., 2005, Chromatographia, 61 (9-10), 505–513.

[4] Li, H-P., Li, G-C., and Jen, J-F., 2004, J. Chin. Chem. Soc., 51 (3), 531–542.

[5] Huang, Z., Li, Y., Chen, B., and Yao, S., 2007, J. Chromatogr. B, 853 (1-2), 154–162.

[6] Yang, X., Xu, D.C., Qiu, J.W., Zhang, H., Zhang, Y.C., Dong, A.J., Ma, Y., and Wang, J., 2009, Chem. Pap., 63 (1), 39–46.

[7] Eurachem Guide, 1998, The Fitness for Purpose of Analytical Methods: A laboratory guide to Method Validation and Related Topics.

[8] Guidelines for validation of analytical methods for non-agricultural pesticide active ingredients and products, http://www.hse.gov.uk/biocides/copr/pdfs/validation.pdf

[9] Metoda Pengujian Residu Pestisida Dalam Hasil Pertanian, Direktorat Jenderal Tanaman Pangan Direktorat Perlindungan Tanaman, 2006.

[10] López-Mesas, M., Crespi, M., Brach, J., and Mullender, J.P., 2000, J. Chromatogr. Sci., 38 (12), 551–555.

[11] Frenich, A.G., J.L. Vidal, J.L., M. Frias, M.M., Ollea-Serrano F., and Olea, N., 2000, J. Mass Spectrom., 35 (8), 967–975.

[12] Szepesy, L., 2000, Chromatographia Suppl., 51 (1), S-98–S-99.

[13] Chang, K.H., 2011, Health Environ. J., 2 (1), 38–43.

[14] Fenoll, J., Helin, P., Lopez, J., Gonzalez, A., and Flores, P., 2007, J. AOAC Int., 90 (6), 1670–1676.

[15] Kujawski, M.W., and Namieśnik, J., 2011, Food Control, 22 (6), 914–919.

[16] Tiryaki, O., 2006, Accredit. Qual. Assur., 11 (10), 506–513.

[17] Horwitz, W., Kamps, L.R., and Boyer, K.W., 1980, J. Assoc. Off. Anal. Chem., 63 (6), 1344–1354.

[18] Thompson, M., 2004, The amazing Horwitz function, amc Technical Brief No.17, Royal Society of Chemistry.

[19] Thompson, M., 2005, What is Proficiency testing? Guide for end-users of chemical data, amc background paper No. 2, The Royal Society of Chemistry.

[20] Gilbert J., and Patey, A.L., 1998, Pure Appl. Chem., 70 (12), 2309–2312.

[21] Dayarathna, D., Thirimanna, C., Mubarak, A., Mackay, L., Rogerson, J., Tang, H., Feng, J., Sin, D.W.M., and Wong, Y-C., 2013, Food Res. Int., 53 (2), 931–937.

[22] Hegazy, R., Mohamed, M.I., and Abu-Sinna, A., 2010, MĀPAN-J. Metrol. Soc. India, 25 (2), 107–113.

[23] Mann I., and Brookman, B., 2011, Selection, Use and Interpretation of Proficiency Testing (PT) Schemes, 2nd ed., Eurachem.

[24] Thompson, M., Ellison, S.L.R., and Wood, R., 2006, Pure Appl. Chem., 78 (1), 145–196.

[25] Hund, E., Massart, D.L., and Smeyers-Verbeke, J., 2000, Anal. Chim. Acta, 423 (2), 145–165.



DOI: https://doi.org/10.22146/ijc.21262

Article Metrics

Abstract views : 1606 | views : 1946


Copyright (c) 2014 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.