IN VITRO INHIBITION OF CELERY (Apium graveolens L.) EXTRACT ON THE ACTIVITY OF XANTHINE OXIDASE AND DETERMINATION OF ITS ACTIVE COMPOUND

https://doi.org/10.22146/ijc.21338

Dyah Iswantini(1*), Tuti Hayati Ramdhani(2), Latifah K Darusman(3)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, 16680
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, 16680
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, 16680
(*) Corresponding Author

Abstract


The objective of this study was to determine the inhibition effect of celery extracts toward xanthine oxidase by in vitro method, and its active compounds. Roots and herb of celery were extracted using water and ethanol solvents. Results indicated that the herbal ethanol extract had the highest inhibition effect (91.40%) at 1400 ppm. The components contained in the herbal ethanol extract were then separated by column chromatography using the best eluent (chloroform : ethyl acetate at 7:3). All of the fractions had inhibition effect greater than 50%. The fraction number 4 was the one with the highest inhibition effect followed by fraction 5 with inhibition percentage of both fractions at 200 ppm were 88.62 and 85.44%, respectively. The analysis of the ultraviolet spectrum of fraction 4 showed the presence of π-π* transition which was resulted by the aromatic C=C, -OH, and C-O chromophores, and also showing the n-σ* transition which was given by -C=O chromophore. The infrared spectrum analysis indicated the presence of aromatic -C=C, -OH, and C=O functional groups. Based on the phytochemical assay and both instrumental spectrums, it was thought that the active compounds of fraction 4 and 5 were in the flavonoid group.

Keywords


Celery (Apium graveolens L.); xanthine oxidase; inhibition; in vitro; active compound

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References

[1] Chang, W.S., Lee, Y.J., Lu, F.J., and Chiang, H.C., 1993, Anticancer Res., 13, 6A, 2165–2170.

[2] Hatano, T., Yashuhara, T., Fukuda, T., Noro, T., and Okuda, T., 1989, Chem. Pharm. Bull., 37, 11, 3005–3009.

[3] Chang, W.S., and Chiang, H.C., 1995, Anticancer Res., 15, 5B, 1969–1974.

[4] Sheu, S.Y., and Chiang, H.C., 1996, Anticancer Res., 16, 1, 311–316.

[5] Lewis, A.S., Murphy, L., Calla, C., Fleary, M., and Purcell, S., 1984, J. Biol. Chem., 259, 1, 12–15.

[6] Havsteen, B., 1983, Biochem. Pharmacol., 32, 7, 1141–1148.

[7] Lio, M., Yoshioka, A., Imayoshi, Y., Koriyama, C., and Moriyama, A., 1984, Agric. Biol. Chem., 48, 6, 1559–1563.

[8] Pirouzpanah, S., Rashidi, M.R., Delazar, A., Razavieh S.V., and Hamidi, A.A., 2009, Iranian J. Pharm. Sci., 5, 3, 163–170.

[9] Nguyen, M.T., Awale, S., Tezuka, Y., Tran, Q.L., Watanabe, H., and Kadota, S., 2004, Biol. Pharm. Bull., 27, 9, 1414–1421.

[10] Kolarovic, J., Popovic, M., Mikov, M., Mitic, R., and Gvozdenovic, L., 2009, Molecules, 14, 4, 1627–1638.

[11] Soedibyo, M., 1998, Alam Sumber Kesehatan, Balai Pustaka, Jakarta.

[12] Harborne, J.B., 1987, Phytochemical Methods, Chapman and Hall, London.

[13] Finney D., 1971, Probit Analysis, Cambrige: Cambrige University Press.

[14] Tamta, H., Kalra, S., and Mukhopadhyay, A.K., 2005, J. Enzyme Inhib. Med. Chem., 20, 317–324.

[15] Cos, P., Ying, L., Calomme, M., Hu, J.P., Cimanga, K., Poel, B.V., Pieters, L., Vlietinck, A.J., and Berghe, D.V., 1998, J. Nat. Prod., 61, 71–76.

[16] Meyer, B.N., Ferigin, N.R., Putnan, J.E., Jacobsen, L.B., Nichols, D.E., and McLaughlin, J.L., 1982, Planta Med., 45, 5, 31–34.

[17] Apaya, K.L., and Chichioco-Hernandez C.L., 2011, J. Med. Plant. Res., 5, 2, 289–292.

[18] Noro, T., Oda, Y., Miyase, T., Ueno, A., and Fukushima, S., 1983, Chem. Pharm. Bull., 31, 3984–3987.

[19] Schemeda-Hirschmannm, G., Loyola, J.I., Sierra, J., Retamal, R., and Rohdriguez, J., 1992, Phytother. Res., 6, 4, 184–188.

[20] Lin, C-M., Chen, C-S., Chen, C-T., Liang, Y-C., and Lin, J-K., 2002, Biochem. Biophys. Res. Commun., 294, 1, 167–172.

[21] Ozyürek, M., Bektaşoğlu, B., Güçlü, K., and Apak, R., 2009, Anal. Chim. Acta, 636, 42–50.

[22] Nessa, Fazilatun, Ismail, Zhari, Mohamed, Nornisah., 2010, Pharm. Biol, 48, 12, 1405–1412.

[23] Silva, M.P., Mira, L., Lima, J., Manso, C.F., 1996, Environ. Toxicol. Pharmacol., 1, 4, 279–284.



DOI: https://doi.org/10.22146/ijc.21338

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