Hendry Yanuar(1*)

(1) Chemistry Dept - FMIPA, Cenderawasih State University, Jayapura- Papua
(*) Corresponding Author


Acetylcholinesterase (AchE) is an enzyme, which work on acetylcholine hydrolysis. Some insecticides can inhibit the activities of this enzyme. The purpose of this research was determined the kinetics of the AchE inhibition over the organophosphate and carbamat inhibitors with the methylindoxylacetate (MIA) as a substrate. The AchE extract was obtained from the local honeybee head (100 heads on the 5 mL of phosphate buffer 0.05 M). Based on the preliminary analysis, the volume of the enzyme extract for the reaction rate was 100 mL on 1-5 mL of the substrate. Monocrothopos, Carbophenathion, Baycarb and MIPC were used as inhibitors which the concentration were 0.0018, 0.0030, and 0.0042 mg/mL respectively. The reaction rate were measured by Fluorescence HPLC Monitor (Shimadzu RF 535) at 540 nm, and some computational program were used on data analysis. The result of this research showed that the maximum rate of MIA hydrolysis by AchE  without the presence of inhibitor was 5.16 mL/s and the hydrolysis constant (Km) was 3.49, and the inhibitors did not influence the maximum rate of substrate hydrolysis. It was finally concluded that the kinetics of AchE inhibition on MIA hydrolysis over the organophosphate and carbamat inhibitors was the competitive inhibition.


acetylcholinesterase; inhibition; organophosphate; carbamat

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[1] Falah, Iip Izul, 1994, Activation, Hydrolysis and Ezyme Inhibition Kinetics in Pre and Post Chromathographic Derivation of Organophosphate and Carbamat Pesticides”, Dessertation, GMU Yogyakarta.

[2] Segel, I.H., 1975, ”Enzyme Kinetics”, John Wiley and Sons Inc., New York.

[3] Rossenberry, T.L., Chang, H.W., and Chen, Y.Y., 1972, Purification of Acetylcholinesterase by Affinity Chromathography and Determination of Active Site Stoichiometry, J. Biol. Chem., 247; 1555 – 1565.


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Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

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