Complex Synthesis of Cis-[Pt(Asc) (NH3)2] and its Effect on Human Breast Cancer MCF-7 Cell in vitro

https://doi.org/10.22146/ijc.35628

Mohammed A. Al-Dolaymi(1*), Khalid F. Al-Rawi(2), Firas T. Al-Shamary(3), Mohammad M.F. Al-Halbosiy(4)

(1) Department of Chemistry, College of Science, University of Anbar, Ramadi, Iraq
(2) Department of Chemistry, College of Science, University of Anbar, Ramadi, Iraq
(3) Department of Chemistry, College of Science, University of Tikrit, Iraq
(4) Biotechnology Research Center, Al-Nahrain University, Iraq
(*) Corresponding Author

Abstract


Bosom malignancy is the most regularly analyzed disease and the imperative reason for growth-related passing among ladies, accounting for 23% of all new tumor cases and 14% of tumor passing's. L-Ascorbic acid, commonly known as vitamin C is well-known in chemistry since long back. It has tremendous medical applications in several diseases. Therefore, in this paper five concentrations of complex cis-[Pt(Asc)(NH3)2] where Asc=L-ascorbic acid derivative on MCF-7 cell line to detect the changes in five cellular parameters (nuclear intensity, mitochondrial membrane potential, valid cell count, cytochrome C, and membrane permeability) after exposure with 24 h are investigated. The results showed that 400 μg/mL has the highest significant effect on the five parameters (nuclear intensity, mitochondrial membrane potential, valid cell count, cytochrome C, and membrane permeability) when compared with Doxorubicin 20 μM (substance used as anti-cancer) which represent the positive control. Also, the 200 μg/mL showed results close to those of the untreated cells which represent the negative control (-ve) with a very few significant differences.

Keywords


breast cancer; vitamin C; cis-platinum; high-content screening

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DOI: https://doi.org/10.22146/ijc.35628

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