Inhibitive Determination of Hg(II) in Aqueous Solution Using Urease Amperometric Biosensor

Dhony Hermanto; Bambang Kuswandi; Dwi Siswanta; Mudasir Mudasir

doi:10.22146/ijc.40617

Inhibitive Determination of Hg(II) in Aqueous Solution Using Urease Amperometric Biosensor

https://doi.org/10.22146/ijc.40617

Dhony Hermanto⁽¹⁾, Bambang Kuswandi⁽²⁾, Dwi Siswanta⁽³⁾, Mudasir Mudasir^(4*)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Mataram, Jl. Majapahit No. 62, Mataram 83125, West Nusa Tenggara, Indonesia
(2) Chemo and Biosensor Group, Faculty of Pharmacy, University of Jember, Jl. Kalimantan No. 37, Jember 68121, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract

An amperometric biosensor for the indirect determination of Hg(II) has been developed based on inhibition of urease (EC 3.5.1.5) immobilized into alginate–chitosan polyelectrolyte complexes membrane. The biosensor response was monitored by following the reduction peak of hydrolyzed urea at around -0.15 V. The amperometric biosensor has a dynamic range 40–90 ppb Hg(II) with limit of detection of 66.45 ppb toward Hg(II) ions, repeatability (CV) value of 0.86% and only Ag(I) as the main potential interference. The sensor shows a stable and reproducible response for more than 2 weeks when it stored dry at 4 °C. The analytical results of Hg(II)-spiked water sample showed a good agreement with those obtained by atomic absorption spectrometry method, suggesting that the developed method may be applied in the determination of Hg(II) in the water samples.

Keywords

amperometric biosensor; urease; alginate–chitosan; inhibition; Hg(II) ion

Full Text:

Full Text PDF

References

[1] Majid, S., El Rhazi, M., Amine, A., and Brett, C.M.A., 2002, An amperometric method for the determination of trace mercury(II) by the formation of complexes with l-tyrosine, Anal. Chim. Acta, 464 (1), 123–133.

[2] Jan, A.T., Azam, M., Siddiqui, K., Ali, A., and Choi, I., 2015, Heavy metals and human health: Mechanistic insight into toxicity and counter defense system of antioxidants, Int. J. Mol. Sci., 16 (12), 29592–29630.

[3] Mahurpawar, M., 2015, Effects of heavy metals on human health, Int. J. Res. Granthaalayah, 530, 1–7.

[4] Teaf, C.M., and Garber, M., 2012, Mercury exposure considerations: Evaluating the chemical form and activities of the individual, Proceedings of the Annual International Conference on Soils, Sediments, Water and Energy, 17 (5), 25–43.

[5] Nordberg, G., Jin, T., Leffler, P., Svensson, M., Zhou, T., and Nordberg, M., 2000, Metallothioneins and diseases with special reference to cadmium poisoning, Analusis, 28 (5), 396–400.

[6] Szkoda, J., Żmudzki, J., and Grzebalska, A., 2006, Determination of total mercury in biological material by atomic absorption spectrometry method, Bull. Vet. Inst. Pulawy, 50, 363–366.

[7] Nixon, D.E., Burritt, M.F., and Moyer, T.P., 1999, The determination of mercury in whole blood and urine by inductively coupled plasma mass spectrometry, S Spectrochim. Acta, Part B, 54 (8), 1141–1153.

[8] Pujol, L., Evrard, D., Groenen-Serrano, K., Freyssinier, M., Ruffien-Cizsak, A., and Gros, P., 2014, Electrochemical sensors and devices for heavy metals assay in water: the French groups’ contribution, Front. Chem., 2, 19.

[9] Elsebai, A.B., Ghica, M.E., Abbas, M.N., and Brett, C.M.A., 2017, Catalase based hydrogen peroxide biosensor for mercury determination by inhibition measurements, J. Hazard. Mater., 340, 344–350.

[10] Somerset, V., Leaner, J., Mason, R., Iwuoha, E., and Morrin, A., 2010, Determination of inorganic mercury using a polyaniline and polyaniline-methylene blue coated screen-printed carbon electrode, Int. J. Environ. Anal. Chem., 90 (9), 671–685.

[11] Turdean, G.L., 2011, Design and development of biosensors for the detection of heavy metal toxicity, Int. J. Electrochem., 2011, 343125.

[12] Domínguez-Renedo, O., Alonso-Lomillo, M.A., Ferreira-Gonçalves, L., and Arcos-Martínez, M.J., 2009, Development of urease based amperometric biosensors for the inhibitive determination of Hg(II), Talanta, 79 (5), 1306–1310.

[13] Kuswandi, B., 2003, Simple optical fiber biosensor based on the immobilized enzyme for monitoring of trace heavy metal ions, Anal. Bioanal. Chem., 376 (7), 1104–1110.

[14] Martinez-Neira, R., Kekic, M., Nicolau, D., and dos Remedios, C.G., 2005, A novel biosensor for mercuric ions based on motor proteins, Biosens. Bioelectron., 20 (7), 1428–1432.

[15] Yabuki, S., 2011, Polyelectrolyte complex membranes for immobilizing biomolecules, and their applications to bio-analysis, Anal. Sci., 27 (7), 695.

[16] Kulig, D., Zimoch-Korzycka, A., Jarmoluk, A., and Marycz, K., 2016, Study on alginate-chitosan complex formed with different polymers ratio, Polymers, 8 (5), 167.

[17] Kuswandi, B., 2008, Affinity biosensor based on a screen-printed electrode modified with DNA for genotoxic compounds detection, Indones. J. Chem., 8 (2), 125–129.

[18] Kuswandi, B., and Mascini, M., 2005, Enzyme inhibition based biosensors for environmental monitoring, Curr. Enzym. Inhib., 1 (3), 207–221.

[19] Singh, M., Verma, N., Garg, A.K., and Redhu, N., 2008, Urea biosensors, Sens. Actuators, B, 134, 345–351.

[20] Engelking, L.R., 2015, “Enzyme Kinetics” in Veterinary Physiological Chemistry, 3^rd ed., Academic Press, 32–38.

[21] Azmi, N.E., Abdullah, J., Ahmad, M., Sidek, H., Heng, L.Y., and Rahman, S.A., 2012, An optical-based biosensor for the determination of ammonium in an aqueous environment, Am. J. Anal. Chem., 3, 364–370.

DOI: https://doi.org/10.22146/ijc.40617