* Corresponding Author
The Performance of a Fixed-Bed Anaerobic Bioreactor Using Sulfate-Reducing Bacterial Consortium from Sikidang Crater Sediments
https://doi.org/10.22146/ijc.45164 Andriyanto Andriyanto(1), Wahyu Wilopo(2), Endah Retnaningrum(3*)
(1) Study Program of Biology Education, STKIP YPM Bangko, Jl. Jenderal Sudirman Km. 02 Bangko, Jambi 37311, Indonesia; Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(2) Geological Engineering Department, Faculty of Engineering, Universitas Gadjah Mada, Jl. Grafika No. 2, Bulaksumur, Yogyakarta 55281, Indonesia
(3) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author
Abstract
This research explored the performance of a fixed-bed anaerobic bioreactor system (FBR) using sulfate-reducing bacteria (SRB) from the sediment of Sikidang Crater in Indonesia. Indonesian natural zeolite was used as an inert medium in this bioreactor system. This bioreactor performance was analyzed based on its sulfate reduction efficiency, Cu removal, pH profile, SRB growth, and the changes in mineral composition of the zeolite surface. Based on a batch experiment, the FBR system was operated at 30 °C with a hydraulic retention time (HRT) of 7 days using a zeolite dose of 100 g/L. After its operation, a large amount of SRB (up to 1.5 × 105 cells/mm2) was entrapped and present in the zeolite. This bacterial consortium could reduce sulfate and copper by around 68% and 99.96%, respectively. In addition, the pH value of the bioreactor changed to neutral, which indicated a good performance of the operation. The result of the Energy-Dispersive X-ray (EDX) confirmed that copper removal was caused by the formation of copper-sulfide precipitation. Mapping also revealed that both copper and sulfur were precipitated at the same location.
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[1] Jamal, A., Yadav, H.L., and Pandey, S.S., 2015, Heavy metals from acid mine drainage in coal mines-A case study, Eur. J. Adv. Eng. Technol., 2 (8), 16–20.
[2] Hurtado, C., Viedma, P., and Cotoras, D., 2018, Design of a bioprocess for metal and sulfate removal from acid mine drainage, Hydrometallurgy, 180, 72–77.
[3] Larrson, M., Nosrati, A., Kaur, S., Wagner, J., Baus, U., and Nydén, M., 2017, Copper removal from acid mine drainage-polluted water using glutaraldehyde-polyethyleneimine modified diatomaceous earth particles, Heliyon, 4 (2), e00520.
[4] Park, I., Tabelin, C.B., Jeon, S., Li, X., Seno, K., Ito, M., and Hiroyosi, N., 2019, A review of recent strategies for acid mine drainage prevention and mine tailings recycling, Chemosphere, 219, 588–606.
[5] Najib, T., Solgi, M., Farazmand, A., Heydarian, S.Y., and Nasernejad, B., 2017, Optimization of sulfate removal by sulfate reducing bacteria using response surface methodology and heavy metal removal in a sulfidogenic UASB reactor, J. Environ. Chem. Eng., 5 (4), 3256–3265.
[6] Kumar, A., Bisht, B.S., Josdhi, V.D., and Dhewa, T., 2011, Review on bioremediation of polluted environment: A management tool, Int. J. Environ. Sci., 1 (16), 1079–1093.
[7] Benedetto, J.S., de Almeida, S.K., Gomes, H.A., Vazoller, R.F., and Ladeira, A.C.Q., 2005, Monitoring of sulfate-reducing bacteria in acid water from uranium mines, Min. Eng., 18 (13-14), 1341–1343.
[8] Gadd, G.M., and White, C., 1993, Microbial treatment of metal pollution-A working biotechnology?, Trends Biotechnol., 11 (8), 353–359.
[9] de Aquino, S., Fuess, L.T., and Pires, E.C., 2017, Media arrangement impacts cell growth in anaerobic fixed-bed reactors treating sugarcane vinasse: Structured vs. randomic biomass immobilization, Bioresour. Technol., 235, 219–228.
[10] Kousi, P., Remoundaki, E., Hatzikioseyian, A., Battaglia-Brunet, F., Joulian, C., Kousteni, V., and Tsezos, M., 2011, Metal precipitation in an ethanol-fed, fixed-bed sulphate-reducing bioreactor, J. Hazard. Mater., 189 (3), 677–684.
[11] Pandey, S., and Sarkar, S., 2017, Anaerobic treatment of wastewater using a two-stage packed-bed reactor containing polyvinyl alcohol gel beads as biofilm carrier, J. Environ. Chem. Eng., 5 (2), 1575–1585.
[12] Kumar, G., and Buitrón, G., 2017, Fermentative biohydrogen production in fixed bed reactors using ceramic and polyethylene carriers as supporting material, Energy Procedia, 142, 743–748.
[13] Muri, P., Marinšek-Logar, R., Djinović, P., and Pintar, A., 2018, Influence of support materials on continuous hydrogen production in anaerobic packed-bed reactor with immobilized hydrogen producing bacteria at acidic condition, Enzyme Microb. Technol., 111, 87–96.
[14] Zheng, H., Li, D., Stanislaus, M.S., Zhang, N., Zhu, Q., Hu, X., and Yang, Y., 2015, Development of a bio-zeolite fixed-bed bioreactor for mitigating ammonia inhibition of anaerobic digestion with extremely high ammonium concentration livestock waste, Chem. Eng. J., 280, 106–114.
[15] Chen, W.S., Tsai, C.Y., Chen, S.Y., Sung, S., and Lin, J.G., 2019, Treatment of campus domestic wastewater using ambient-temperature anaerobic fluidized membrane bioreactors with zeolites as carriers, Int. Biodeterior. Biodegrad., 136, 49–54.
[16] Retnaningrum, E., and Wilopo, W., 2017, Removal of sulphate and manganese on synthetic wastewater in sulphate reducing bioreactor using Indonesian natural zeolite, Indones. J. Chem., 17 (2), 203–210.
[17] Encina, P.A.G., and Hidalgo, M.D., 2005, Influence of substrate feed patterns on biofilm development in anaerobic fluidized bed reactors (AFBR), Process Biochem., 40 (7), 2509–2516.
[18] Sheoran, A.S., Sheoran, V., and Choudhary, R.P., 2010, Bioremediation of acid-rock drainage by sulphate-reducing prokaryotes: A review, Miner. Eng., 23 (14), 1073–1100.
[19] Zhang, M., Wang, H., and Han, X., 2016, Preparation of metal-resistant immobilized sulfate reducing bacteria beads for acid mine drainage treatment, Chemosphere, 154, 215–223.
[20] Ahmad, M., Liu, S., Mahmood, N., Mahmood, A., Ali, M., Zheng, M., and Ni, J., 2017, Effects of porous carrier size on biofilm development, microbial distribution and nitrogen removal in microaerobic bioreactors, Bioresour. Technol., 234, 360–369.
[21] Wijesinghe, D.T.N., Dassanayake, K.B., Scales, P.J., Sommer, S.G., and Chen, D., 2018, Effect of Australian zeolite on methane production and ammonium removal during anaerobic digestion of swine manure, J. Environ. Chem. Eng., 6 (1), 1233–1241.
[22] Kaksonen, A.H., Plumb, J.J., Robertson, W.J., Vanhanen, M.R., Franzman, P.D., and Puhakka, J.A., 2006, The performance, kinetics and microbiology of sulfidogenic fluidized-bed treatment of acidic metal- and sulfate-containing wastewater, Hydrometallurgy, 83 (1-4), 204–213.
[23] Gupta, G.N., Srivastava, S., Khare, S.K., and Prakash, V., 2014, Extremophiles: An overview of microorganism from extreme environment, Int. J. Agric. Environ. Biotechnol., 7 (2), 371–380.
[24] Kolmert, Å., Wikström, P., and Hallberg, K.B., 2000, A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures, J. Microbiol. Methods, 41 (3), 179–184.
[25] Postgate, J.R., 1984, The Sulphate Reducing Bacteria, 2nd Ed., University Press, Cambridge, UK, 20–30.
[26] Cardell, C., and Guerra, I., 2016, An overview of emerging hyphenated SEM-EDX and Raman spectroscopy systems: Applications in life, environmental and materials sciences, TrAC, Trends Anal. Chem., 77, 156–166.
[27] Cabrera, G., Pérez, R., Gómez, J.M., Abalos, A., and Cantero, D., 2006, Toxic effects of dissolved heavy metals on Desulfovibrio vulgaris and Desulfovibrio sp. strains, J. Hazard. Mater., 135 (1-3), 40–46.
[28] Chen, J., Wang, R., Wang, X., Chen, Z., Feng, X., and Qin, M., 2019, Response of nitrification performance and microbial community structure in sequencing biofilm batch reactors filled with different zeolite and alkalinity ratio, Bioresour. Technol., 273, 487–495.
[29] Song, Z., Zhang, X., Ngo, H.H., Guo, W., Song, P., Zhang, Y., Wen, H., and Guo, J., 2019, Zeolite powder based polyurethane sponges as biocarriers in moving bed biofilm reactor for improving nitrogen removal of municipal wastewater, Sci. Total Environ., 651 (1), 1078–1086.
[30] Li, R., Liu, D., Zhang, Y., Zhou, J., Tsang, Y.F., Liu, Z., Duan, N., and Zhang, Y., 2019, Improved methane production and energy recovery of post hydrothermal liquefaction waste water via integration of zeolite adsorption and anaerobic digestion, Sci. Total Environ., 651 (1), 61–69.
[31] Janyasuthiwong, S., Rene, E.R., Esposito, G., and Lens, P.N.L., 2015, Effect of pH on Cu, Ni and Zn removal by biogenic sulfide precipitation in an inversed fluidized bed bioreactor, Hydrometallurgy, 158, 94–100.
[32] Welch, K., Cai, Y., and Strømme, M.A., 2012, Method for quantitative determination of biofilm viability, J. Funct. Biomater., 3 (2), 418–431.
[33] Retnaningrum, E., and Wilopo, W., 2016, Performance and bacterial composition of anodic biofilms in microbial fuel cell using dairy wastewater, AIP Conf. Proc., 1744 (1), 020018.
[34] Martins, M.M., Faleirob, L., Barros, R.J., Veríssimo, A.R., Barreiros, M.A., and Costa, C.M., 2009, Characterization and activity studies of highly heavy metal resistant sulphate reducing bacteria to be used in acid mine drainage decontamination, J. Hazard. Mater, 166 (2-3), 706–713.
[35] Miran, W., Jang, J., Nawaz, M., Shahzad, A., Jeong, S.E., Jeon, C.O., and Lee, D.S., 2017, Mixed sulfate-reducing bacteria-enriched microbial fuel cells for the treatment of wastewater containing copper, Chemosphere, 189, 134–142.
[36] Raj, K.K., Sardar, U.S., Bhargavi, E., Devi, I., Bhunia, B., and Tiwari, O.N., 2018, Advances in exopolysaccharides based bioremediation of heavy metals in soil and water: A critical review, Carbohydr. Polym., 199, 353–364.
[37] Bratkova, S., Koumanova, B., and Beschkov, V., 2013, Biological treatment of mining wastewaters by fixed-bed bioreactors at high organic loading, Bioresour. Technol., 137, 409–413.
[38] Hullesbusch, E.D., Zandvoort, M.H., and Lens, P.N.L., 2003, Metal immobilisation by biofilms: Mechanisms and analytical tools, Rev. Environ. Sci. Biotechnol, 2 (1), 9–33.
[39] White, C., and Gad, G.M., 2000, Copper accumulation by sulfate-reducing bacterial biofilms, FEMS Microbiol. Lett., 183 (2), 313–318.
[40] Kiran, M.G., Pakshirajan, K., and Das, G., 2017, Heavy metal removal from multicomponent system by sulfate reducing bacteria: Mechanism and cell surface characterization, J. Hazard. Mater., 324, 62–70.
[41] Chang, J.C., 1993, “Solubility product constants” in CRC Hand Book of Chemistry and Physics, Eds. Lide, D.R., CRC Press, Boca Raton, 8–39.
DOI: https://doi.org/10.22146/ijc.45164
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