Antifungal Activity of TiO2/Ag Nanoparticles under Visible Light Irradiation

https://doi.org/10.22146/ijc.49150

Nahzim Rahmat(1*), Endang Tri Wahyuni(2), Adhitasari Suratman(3)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract


The doping of TiO2 by Ag(I) from [Ag(S2O3)2]3– contained in radiophotography wastewater by photoreduction method has been performed. TiO2/AgNPs photocatalyst was examined for its activity as an antifungal material for the inhibition of C. albicans in water under visible light irradiation. In the doping process, the weight of TiO2 was varied to obtain TiO2/AgNPs with different amounts of Ag. The TiO2/AgNPs samples were characterized by using FTIR, SRUV, TEM, SEM-EDX, and XRD methods. The antifungal test was carried out by disc diffusion method under visible light irradiation, wherein the amount of Ag-doped on TiO2, the dose of TiO2/AgNPs, and the irradiation time were optimized. The research results indicated that the antifungal activity of TiO2/AgNPs in inhibiting C. albicans has been successfully prepared. The highest inhibition was achieved by using 0.5 g/L of TiO2/AgNPs (I), at 5 h of irradiation time.

Keywords


TiO2/Ag; antifungal; C. albicans; visible light; nanoparticle

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References

[1] Amala, S.E., and Aleru, C.P., 2016, Bacteriological quality of swimming pools water in Port Harcourt Metropolis, Nat. Sci., 8 (3), 79–84.

[2] Brinkman, N.E., Haugland, R.A., Wymer, L.J., Byappanahalli, M., Whitman, R.L., and Vesper, S.J., 2003, Evaluation of a rapid, quantitative real-time PCR method for enumeration of pathogenic Candida cells in water, Appl. Environ. Microbiol., 69 (3), 1775–1782.

[3] Lubbers, J.D., Chauan, S., and Bianchine, J.R., 1982, Controlled clinical evaluations of chlorine dioxide, chlorite, and chlorate in man, Environ. Health Perspect., 46, 57–62.

[4] Cesar, J., Sumita, T.C., Junqueira, J.C., Jorge, A.O.C., and do Rego, M.A., 2012, Antimicrobial effects of ozonated water on the sanitization of dental instruments contaminated with E. coli, S. aureus, C. albicans, or the spores of B. atrophaeus, J. Infect. Public Health, 5 (4), 269–274.

[5] Polo-López, M.I., Castro-Alférez, M., Oller, I., and Fernández-Ibáñez, P., 2014, Assessment of solar photo-Fenton, photocatalysis, and H2O2 for removal of phytopathogen fungi spores in synthetic and real effluents of urban wastewater, Chem. Eng. J., 257, 122–130.

[6] Tatlıdil, I., Sökmen, M., Breen, C., Clegg, F., Buruk, C.K., and Bacaksiz, E., 2011, Degradation of Candida albicans on TiO2 and Ag-TiO2 thin films prepared by sol–gel and nanosuspensions, J. Sol-Gel Sci. Technol., 60 (1), 23–32.

[7] Wahyuni, E.T., Roto, R., and Prameswari, M., 2019, Antibacterial activity of TiO2/Ag-nanoparticle under visible light, Mater. Sci. Forum, 948, 33–42.

[8] Wahyuni, E.T., Roto, R., Novarita, D., Suwondo, K.P., and Kuswandi, B., 2019, Preparation of TiO2/AgNPs by photodeposition method using Ag(I) present in radiophotography wastewater and their antibacterial activity in visible light irradiation, J. Environ. Chem. Eng., 7 (4), 103178.

[9] Abbad, S., Guergouri, K., Gazaout, S., Djebabra, S., Zertal, A., Barille, R., and Zaabat, M., 2020, Effect of silver doping on the photocatalytic activity of TiO2 nanopowders synthesized by the sol-gel route, J. Environ. Chem. Eng., 8 (3), 103718.

[10] Huang, M., Tso, E., Datye, A.K., Prairie, M.R., and Stange, B.M., 1996, Removal of silver in photographic processing wastewater by TiO2-based photocatalysis, Environ. Sci. Technol., 30 (10), 3084–3088.

[11] Sontakke, S., Mohan, C., Modak, J., and Madras, G., 2012, Visible light photocatalytic inactivation of Escherichia coli with combustion synthesized TiO2, Chem. Eng. J., 189-190, 101–107.

[12] Pelaez, M., Nolan, N.T., Pillai, S.C., Seery, M.K., Falaras, P., Kontos, A.G., Dunlop, P.S.M., Hamilton, J.W.J., Byrne, J.A., O’shea, K., Entezari, M.H., and Dionysiou, D.D., 2012, Review on the visible light active titanium dioxide photocatalysts for environmental applications, Appl. Catal., B, 125, 331–349.

[13] Suwarnkar, M.B., Dhabbe, R.S., Kadam, A.N., and Garadkar, K.M., 2014, Enhanced photocatalytic activity of Ag doped TiO2 nanoparticles synthesized by a microwave assisted method, Ceram. Int., 40 (4), 5489–5496.

[14] Kernazhitsky, L., Shymanovska, V., Gavrilko, T., Naumov, V., Fedorenko, L., Kshnyakin, V., and Baran, J., 2014, Room temperature photoluminescence of anatase and rutile TiO2 powders, J. Lumin., 146, 199–204.

[15] Lenzi, G.G., Fávero, C.V.B., Colpini, L.M.S., Bernabe, H., Baesso, M.L., Specchia, S., and Santo, O.A.A., 2011, Photocatalytic reduction of Hg(II) on TiO2 and Ag/TiO2 prepared by the sol–gel and impregnation methods, Desalination, 270 (1-3), 241–247.

[16] Susanthy, D., Santosa, S.J., and Kunarti, E.S., 2020, Antibacterial activity of silver nanoparticles capped by p-aminobenzoic acid on Escherichia coli and Staphylococcus aureus, Indones. J. Chem., 20 (1), 182–189.

[17] Susanthy, D., Santosa, S.J., and Kunarti, E.S., 2018, The synthesis and stability study of silver nanoparticles prepared using p-aminobenzoic acid as reducing and stabilizing agent, Indones. J. Chem., 18 (3), 421–427.

[18] Roto, R., Marcelina, M., Aprilita, N.H., Mudasir, M., Natsir, T.A., and Mellisani, B., 2017, Investigation on the effect of addition of Fe3+ ion into the colloidal AgNPs in PVA solution and understanding its reaction mechanism, Indones. J. Chem., 17 (3), 439–445.

[19] Roto, R., Rasydta, H.P., Suratman, A., and Aprilita, N.H., 2018, Effect of reducing agents on physical and chemical properties of silver nanoparticles, Indones. J. Chem., 18 (4), 614–620.

[20] Zhao, Y., Sun, L., Xi, M., Feng, Q., Jiang, C., and Fong, H., 2014, Electrospun TiO2 nanofelt surface-decorated with Ag nanoparticles as sensitive and UV-cleanable substrate for surface enhanced Raman scattering, ACS Appl. Mater. Interfaces, 6 (8), 5759–5767.

[21] Albiter, E., Valenzuela, M.A., Alfaro, S., Valvelde-Aguilar, G., and Martinez-Pallares, F.M., 2015, Photocatalytic deposition of Ag nanoparticles on TiO2: Metal precursor effect on the structural and photoactivity properties, J. Saudi Chem. Soc., 19 (5), 563–573.

[22] Flanagan, J.N., and Steck, T.R., 2017, The relationship between agar thickness and antimicrobial susceptibility testing, Indian J. Microbiol., 57 (4), 503–506.

[23] Fujii, G., Chang, J.E., Coley, T., and Steere, B., 1997, The formation of amphotericin B ion channels in lipid bilayers, Biochemistry, 36 (16), 4959–4968.

[24] Mei, S., Wang, H., Wang, W., Tong, L, Pan, H., Ruan, C., Ma, Q., Liu, M., Yang, H., Zhang, L., Cheng, Y., Zhang, Y., Zhao, L., and Chu, P.K., 2014, Antibacterial effects and biocompatibility of titanium surfaces with graded silver incorporation in titania nanotubes, Biomaterials, 35, 4255–4265.



DOI: https://doi.org/10.22146/ijc.49150

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