Anticancer Activity of Synthesized ZnO and ZnO/AgCl Nanocomposites against Five Human Cancer Cells

https://doi.org/10.22146/ijc.76872

Fattima Al-Zahra Gabar Gassim(1*), Ali Jabbar Jasim Makkawi(2)

(1) Department of Pharmaceuticals, College of Pharmacy, University of Babylon, Hilla 51002, Iraq
(2) Department of Chemistry, College of Science, University of Babylon, Ministry of Education, Hilla 51002, Iraq
(*) Corresponding Author

Abstract


In this work, a refluxing method is used to prepare nanoparticles of zinc oxide (ZnO NPS) and zinc oxide /silver chloride nanocomposites (ZnO/AgCl NCS). The structural properties of nanocrystals are studied by Atomic Force Microscope (AFM), X-Ray Diffraction (XRD), and Field Emission Scanning Electron Microscopy (FE-SEM) to determine the average crystal size, morphology, particle size and average grain size of nanocrystals. The results of anticancer efficiency of ZnO NPs and ZnO/AgCl NCs show cytotoxic activity against five human cancer cells, namely hepatocellular carcinoma, rhabdomyosarcoma (RD), colorectal carcinoma (HCT116), mammary gland (MCF-7), and epidermoid carcinoma (Hep2) compared against doxorubicin. The anticancer mechanism of pure and nanocomposites ZnO are suggested according to the release of Zn+2 and the production of reactive oxidation species (ROS) and the effect of these species on cancer inhibition.

Keywords


ZnO nanoparticles; the anticancer activity of nanoparticles; ZnO/AgCl nanocomposites

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References

[1] Ratan, Z.A., Haidere, M. F., Nurunnabi, M., Shahriar, S.M., Ahammad, A.J.S., Shim, Y.Y., Reaney, M.J.T., and Cho, J.Y., 2020, Green chemistry synthesis of silver nanoparticles and their potential anticancer effects, Cancers, 12 (4), 855.‏

[2] Abdolmaleki, A., Asadi, A., Gurushankar, K., Shayan, T.K., and Sarvestani, F.A., 2021, Importance of nano medicine and new drug therapies for cancer, Adv. Pharm. Bull., 11 (3), 450–457.‏

[3] Hussain, A., Oves, M., Alajmi, M.F., Hussain, I., Amir, S., Ahmed, J., Rehman, M.T., El-Seedi, H.R., and Ali, I., 2019, Biogenesis of ZnO nanoparticles using Pandanus odorifer leaf extract: Anticancer and antimicrobial activities, RSC Adv., 9 (27), 15357–15369.‏

[4] Anjum, S., Hashim, M., Malik, S.A., Khan, M., Lorenzo, J.M., Abbasi, B.H., and Hano, C., 2021, Recent advances in zinc oxide nanoparticles (ZnO NPs) for cancer diagnosis, target drug delivery, and treatment, Cancers, 13 (18), 4570.‏

[5] Wang, J., Gao, S., Wang, S., Xu, Z., and Wei, L., 2018, Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy, Int. J. Nanomed., 13, 3441.

[6] Makkawi, A.J.J., Aysa, N.H., and Gassim, F.A.G., 2019, Anticancer activity of zinc oxide and zinc oxide/cadmium sulfide nanocomposites, Asian J. Pharm. Clin. Res., 12 (2), 535–539.

[7] Sawant, V.J., and Bamane, S.R., 2018, PEG-beta-cyclodextrin functionalized zinc oxide nanoparticles show cell imaging with high drug payload and sustained pH responsive delivery of curcumin in to MCF-7 cells, J. Drug Delivery Sci. Technol., 43, 397–408.‏

[8] Safdar Ali, R., Meng, H., and Li, Z., 2022, Zinc-based metal-organic frameworks in drug delivery, cell imaging, and sensing, Molecules, 27 (1), 100.‏

[9] Xiong, H.M., 2013, ZnO nanoparticles are applied to bioimaging and drug delivery, Adv. Mater., 25 (37), 5329–5335.‏

[10] Hashimoto, H., Tanino, R., Nakamura, M., and Fujita, Y., 2015, Surface treatment of zinc oxide nanoparticles by silica coating and evaluation of their dispersibility and photoluminescent properties, e-J. Surf. Sci. Nanotechnol., 13, 451–454.

[11] Khatami, M., Varma, R.S., Zafarnia, N., Yaghoobi, H., Sarani, M., and Kumar, V.G., 2018, Applications of green synthesized Ag, ZnO and Ag/ZnO nanoparticles for making clinical antimicrobial wound-healing bandages, Sustainable Chem. Pharm., 10, 9–15.‏

[12] Ng, C.T., Yong, L.Q., Hande, M.P., Ong, C.N., Yu, L.E., Bay, B.H., and Baeg, G.H., 2017, Zinc oxide nanoparticles exhibit cytotoxicity and genotoxicity through oxidative stress responses in human lung fibroblasts and Drosophila melanogaster, Int. J. Nanomed., 12, 1621–1637.

[13] Chica, A., Gatti, G., Moden, B., Marchese, L., and Iglesia, E., 2006, Selective catalytic oxidation of organosulfur compounds with tert‐butyl hydroperoxide, Chem. - Eur. J., 12 (7), 1960–1967.‏

[14] Aysa, N.H., Al-Maamori, M.H., and Al-Maamori, N.A.A., 2017, Preparation and Surface Modification of Zinc Oxide Nanoparticles, J. Univ. Babylon Pure Appl. Sci., 25 (2), 497–503.‏

[15] Pirhashemi, M., and Habibi-Yangjeh, A., 2014, Preparation of AgCl–ZnO nanocomposites as highly efficient visible-light photocatalysts in water by a one-pot refluxing method, J. Alloys Compd., 601, 1–8.‏

[16] Xiao, F., Xu, T., Lu, B., and Liu, R., 2020, Guidelines for antioxidant assays for food components, Food Front., 1 (1), 60–69.‏

[17] Kavitha, R., 2018, Antidiabetic and enzymatic antioxidant potential from ethanolic extracts of leaf and fruit of Trichosanthes dioica and leaf of Clitoria ternatea on diabetic rats induced by streptozotocin, Asian J. Pharm. Clin. Res., 11 (5), 233–239.‏

[18] Bazant, P., Kuritka, I., Munster, L., and Kalina, L., 2015, Microwave solvothermal decoration of the cellulose surface by nanostructured hybrid Ag/ZnO particles: A joint XPS, XRD and SEM study, Cellulose, 22 (2), 1275–1293.‏

[19] Ai, L., Zhang, C., and Jiang, J., 2013, Hierarchical porous AgCl@Ag hollow architectures: Self-templating synthesis and highly enhanced visible light photocatalytic activity, Appl. Catal., B, 142-143, 744–751.‏

[20] Gabar Gassim, F.A.Z., Makkaw, A.J., and Aysa, N.H., 2021, Removal of mercury(II) in aqueous solution by using ZnO and ZnO/CdS nanoparticles as photocatalysts, Iran. J. Catal., 11 (4), 397–403.‏

[21] Savaloni, H., and Savari, R., 2018, Nano-structural variations of ZnO:N thin films as a function of deposition angle and annealing conditions: XRD, AFM, FESEM and EDS analyses, Mater. Chem. Phys., 214, 402–420.‏

[22] Weiss, C., McLoughlin, P., and Cathcart, H., 2015, Characterisation of dry powder inhaler formulations using atomic force microscopy, Int. J. Pharm., 494 (1), 393–407.

[23] Fan, M., Han, Y., Gao, S., Yan, H., Cao, L., Li, Z., Liang, X.J., and Zhang, J., 2020, Ultrasmall gold nanoparticles in cancer diagnosis and therapy, Theranostics, 10 (11), 4944–4957.‏‏

[24] Diebold, L., and Chandel, N.S., 2016, Mitochondrial ROS regulation of proliferating cells, Free Radical Biol. Med., 100, 86–93.‏

[25] Moghimipour, E., Rezaei, M., Ramezani, Z., Kouchak, M., Amini, M., Angali, K.A., Dorkoosh, F.A., and Handali, S., 2018, Transferrin targeted liposomal 5-fluorouracil induced apoptosis via mitochondria signaling pathway in cancer cells, Life Sci., 194, 104–110.‏

[26] Guo, C., Sun, L., Chen, X., and Zhang, D., 2013, Oxidative stress, mitochondrial damage, and neurodegenerative diseases, Neural Regener. Res., 8 (21), 2003–2014.‏

[27] Jiang, J., Pi, J., and Cai, J., 2018, The advancing of zinc oxide nanoparticles for biomedical applications, Bioinorg. Chem. Appl., 2018, 1062562.‏

[28] Pimpliskar, P.V., Motekar, S.C., Umarji, G.G., Lee, W., and Arbuj, S.S., 2019, Synthesis of silver-loaded ZnO nanorods and their enhanced photocatalytic activity and photoconductivity study, Photochem. Photobiol. Sci., 18 (6), 1503–1511.‏‏



DOI: https://doi.org/10.22146/ijc.76872

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