Uji Sitotoksik Kapang Laut Perairan Nipah Terhadap Artemia salina Leach

https://doi.org/10.22146/jfs.28

Elrade Rofaani(1*), Reni Nur'aini Adiati(2)

(1) Pusat Teknologi Farmasi dan Medika Badan Pengkajian dan Penerapan Teknologi (BPPT)
(2) Departemen Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam-Institut Sains dan Teknologi Nasional
(*) Corresponding Author

Abstract


Test on cytotoxic activity of marine fungi from Nipah waters have been conducted. Each solate was cultured on malt extract liquid for 15 days, and extracted by using ethyl acetate to both pellet and supernatant. Brine Shrimp Lethality Test (BSLT) was applied for cytotoxic test against Artemia salina Leach at concentrations of 10; 100; 500; and 1000 μg/ml. Cytotoxic activity showed that the percentage mortality of the brine shrimp was obtained by 1000 μg/ml at 75-100%, and the LC50 of each extract was varied from 117.21 to 531.65 μg/ml. Furthermore, the lowest LC50 was showed by sample with code number NA 14.3 at 117.21 μg/ml.

Keywords


antioxidant, BSLT, LC50, marine fungi

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References

Anonim. 2004. Ekspedisi potential reports (POTRETS) pulau nipah. http://tisda.bppt.go.id/nipah/nipah-geologi.html. Diakses tanggal 21 Desember 2004.

Lay, B.W. dan S. Hastowo. 1992. Mikrobiologi. CV Rajawali. Jakarta. 376 p.

Carballo, J.L., Z.L. Hernandez-Inda, P. Perez , and M. D. Garcia-Gravales. 2002. Methodology article: a comparison between two brine shrimp assays to detect in vitro citotoxicity in marine natural products. 1472-6750. BMC Biotechnology. Mexico. http://www. Biomedcentral.com/1472-6750/2/17. Diakses tanggal 20 Februari 2006.

Csuros, M. and C. Csuros. 1999. Microbiological examination of water and wastewater. Lewis Publishers. Boca Raton London New York Washington, D.C. 70-72.

Finney, D.L. 1971. Probit analysis. 3rd Edition, Canbridge University Press. London. 333 p.

Hostettmann, K. 1991. Methods in plant biochemistry assays for bioactivity. Institute of Pharmacognosy and Phytochemistry. University of Lausanne. Switzerland. London. 6:8-33.

Judoamijojo, M. 1992. Teknologi fermentasi. PAU Bioteknologi Institut Pertanian Bogor. Bogor. 272-273.

Kohlmeyer, J. and E. Kohlmeyer. 1979. Marine mycology-the higher fungi. Academic Press. New York. 690 p.

Meyer, B.N. et al. 1982. Brine shrimp: a convenient general bioassay for active plant constituent. Planta Medica. Braunschweig. 31-34.

Raquel, C.J. 2002. Isolation and structure elucidation of bioactive secondary metabolites from marine sponges and sponge-derived fungi. Dissertation of Doctoral. Wurzburg University. Germany. 9-90.

Scheuer, P.J. 1987. Biorganic marine chemistry. Vol. I. Spinger-Verlag, Berlin Heidelberg. 20-34.

Panggabean, M.G.L. 1984. Teknik penetasan dan pemanenan Artemia salina. Oseana. Jakarta: IX(2):1-4.



DOI: https://doi.org/10.22146/jfs.28

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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