Produksi dan Profil Kimia Hidrolisat Protein dari Hasil Samping Pengolahan Udang Segar

https://doi.org/10.22146/jfs.59906

Tatty Yuniarti(1*), Adham Prayudi(2), Lilis Supenti(3), Hendria Suhrawardan(4), Pujo Martosuyono(5)

(1) Politeknik Ahli Usaha Perikanan, Unit Praktik Lapang Komunikasi dan Penyuluh Kampus Bogor, Kota Bogor, Jawa Barat, Indonesia
(2) Politeknik Kelautan dan Perikanan Kota Agung, Lampung, Bandar Lampung, Indonesia
(3) Politeknik Ahli Usaha Perikanan, Unit Praktik Lapang Komunikasi dan Penyuluh Kampus Bogor, Kota Bogor, Jawa Barat, Indonesia
(4) Politeknik Ahli Usaha Perikanan, Unit Praktik Lapang Komunikasi dan Penyuluh Kampus Bogor, Kota Bogor, Jawa Barat, Indonesia
(5) Balai Riset Bioteknologi dan Pengolahan Produk Hasil Perikanan, Jakarta, Indonesia
(*) Corresponding Author

Abstract


Udang merupakan salah satu komoditas hasil perikanan unggulan di Indonesia. Udang diekspor dalam bentuk beku, bentuk olahan, dan bentuk udang segar. Proses pengolahan udang segar menghasilkan hasil samping berupa kepala udang sekitar 68% dan belum dimanfaatkan. Pemanfaatan hasil samping industri pengolahan udang segar adalah pembuatan hidrolisat protein. Penelitian bertujuan untuk menentukan lama waktu hidrolisis optimal dan profil kimia hidrolisat protein dari kepala udang yang diproduksi secara enzimatis. Metode pembuatan hidrolisat kepala udang menggunakan enzim alkalase pada suhu 55°C, konsentrasi enzim 20,000 unit/kg substrat selama 7 jam. Parameter yang diamati adalah derajat hidrolisis (DH), rendemen, analisa proksimat dan asam amino pada bahan baku dan produk hidrolisat udang. Nilai DH kepala udang selama 7 jam adalah 61,33%±3,67. Rendemen hidrolisat kepala udang adalah 79,20%. Kandungan protein bahan baku dan hidrolisat kepala udang 10,52±0,08%; 3,71±0,08%. Bahan baku dan hidrolisat kepala udang mengandung asam amino 21,12% dan 3,33% bb yang didominasi oleh asam amino non esensial seperti asam glutamat (0,5% b/b), dan asam amino esensial leusin (0,30% b/b) dan lisin (0,24% b/b). Kesimpulan penelitian adalah hidrolisat kepala udang berpotensi untuk dimanfaatkan sebagai ingredien bahan pangan yang kaya asam amino.

Keywords


Asam amino; derajat hidrolisis; kepala udang

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References

AOAC. 2005. Association of officiating analytical chemists 18th edition, Washington DC, Method 935.14 and 992.24.

Auwal, S.M., M. Zarei, A. Abdul-Hamid & N. Saari. 2017. Optimization of bromelain-aided production of angiotensin i-converting enzyme inhibitory hydrolysates from stone fish using response surface methodology. Marine Drugs. 15 (4): 104 doi:10.3390/md15040104.

Cahú, T.B., SD. Santos, A. Mendes, C.R. Córdula, S.F. Chavante, L.B. Carvalho, H.B. Nader & R.S. Bezerra. 2012. Recovery of protein, chitin, carotenoids and glycosaminoglycans from pacific white shrimp (Litopenaeus vannamei) processing waste. Process Biochem. 47: 570-577. https://doi.org/10.1016/j.procbio.2011.12.012

Cao, W., C. Zhang, P. Hong, H. Ji, J. Hao & J. Zhang. 2009. Autolysis of shrimp head by gradual temperature and nutritional quality of the resulting hydrolysate. LWT - Food Sci. Technol. 42: 244-249. https://doi.org/10.1016/j.lwt.2008.05.026

Chai, X., W. Wu, C. Chen, X. Duan, H. Yu & X. Liu. 2020. Physical and oxidative stability of chicken oil-in-water emulsion stabilized by chicken protein hydrolysates. Food Sci. Nutr. 8: 371-378. https://doi.org/10.1002/fsn3.1316

Dey, S.S & K.C. Dora. 2014. Optimization of the production of shrimp waste protein hydrolysate using microbial proteases adopting response surface methodology. J Food Sci Technol. 51: 16-24. https://doi.org/10.1007/s13197-011-0455-4

Dhanabalan, V., M. Xavier, L.N. Murthy, K.K. Asha, A.K. Balangea & B.B. Nayak. 2020. Evaluation of physicochemical and functional properties of spray-dried protein hydrolysate from non-penaeid shrimp (Acetes indicus). J. Sci. Food Agric. 100: 50-58. https://doi.org/10.1002/jsfa.9992

Guo, X., X. Han, Y. He, H. Du & Z. Tan. 2014. Optimization of enzymatic hydrolysis for preparation of shrimp flavor precursor using response surface methodology. J. Food Qual. 37: 229-236. https://doi.org/10.1111/jfq.12091

Kandra, P & M.M. Challa., 2012. Efficient use of shrimp waste: Present and future trends. Appl. Microbiol Biotechnol. 17-29. https://doi.org/10.1007/s00253-011-3651-2

Kirimura, J., A. Shimizu, A. Kimizuka, T. Ninomiya & N. Katsuya. 1969. Contribution of peptides and amino acids to the taste of foods. J. Agric. Food Chem. 17: 689-695. https://doi.org/https://doi.org/10.1021/jf60164a031

Klomklao, S & S. Benjakul. 2018. Protein hydrolysates prepared from the viscera of skipjack tuna (Katsuwonus pelmamis): Antioxidative activity and functional properties. Turkish J. Fish. Aquat. Sci. 18: 69-79. https://doi.org/10.4194/1303-2712-v18

Limam, Z., S. Sadok & A. El. Abed. 2008. Enzymatic hydrolysis of shrimp head waste: functional and biochemical properties. Food Biotechnol. 22: 37-41. https://doi.org/10.1080/08905430802458461

Mackay, J.A & A. Chilkot. 2010. Temperature sensitive peptides: Engineering hyperthermia - directed therapeutics. International Journal Hyperth. 24. https://doi.org/10.1080/02656730802149570.

Madende, M & M. Hayes. 2020. Fish by-product use as biostimulants: An overview of the current state of the art , including relevant legislation and regulations within the eu and USA. Molecules 25: 21-20.

Manninen, A.H. 2009. Protein hydrolysates in sports nutrition. Nutr. Metab. (Lond). 6: 1-5. https://doi.org/10.1186/1743-7075-6-38

Marcet, I., C. Álvarez, B. Paredes & M. Díaz. 2016. The use of sub-critical water hydrolysis for the recovery of peptides and free amino acids from food processing wastes . Review of sources and main parameters. Waste Manag. 49: 364371. https://doi.org/10.1016/j.wasman.2016.01.009

Martosuyono, P., Y.N. Fawzya, G. Patantis & S. Sugiyono, 2019. Enzymatic production of fish protein hydrolysates in a pilot plant scale. Squalen Bull. Mar. Fish. Postharvest Biotechnol. 14: 85-92.

Mashari, S., R. Nurmalina & S. Suharno, 2019. Dinamika daya saing ekspor udang beku dan olahan Indonesia di pasar internasional. J. Agribisnis Indones. 7: 37–52.

Mccarthy, A.L., Y.C.O. Callaghan & N.M.O. Brien. 2013. Protein hydrolysates from agricultural crops-bioactivity and potential for functional food development. Agriculture. 3: 112-130. https://doi.org/10.3390/agriculture3010112

Mirzah, Filawati, 2014. Pengolahan Limbah Udang untuk Memperoleh Bahan Pakan Sumber Protein Hewani Pengganti Tepung Ika. J. Peternak. Indones. 15, 52–61.

Mizani, M., M. Aminlari & M. Khodabandeh. 2005. An effective method for producing a nutritive protein extract powder from shrimp-head waste. Food Sci. Technol. Int. 11: 49-54. https://doi.org/10.1177/1082013205051271

Nurdiawati, A., C. Suherman, Y. Maxiselly, M. Ali, A. Bayu & A. Purwoko. 2019. Liquid feather protein hydrolysate as a potential fertilizer to increase growth and yield of patchouli (Pogostemon cablin Benth) and mung bean (Vigna radiata). Int. J. Recycl. Org. Waste Agric. 8: 221-232. https://doi.org/10.1007/s40093-019-0245-y

Petrova, I., I. Tolstorebrov & T. Magne. 2018. Production of fish protein hydrolysates step by step: Technological aspects, equipment used, major energy costs and methods of their minimizing. Int. Aquat. Res. 10: 223-241. https://doi.org/10.1007/s40071-018-0207-4

Randriamahatodya, Z., K.S.B. Syllaa, H.T.M. Nguyena, C. Donnay-Morenoa, L. Razanamparany, N. Bourgougnon & J.P. Bergéa. 2011. Proteolysis of shrimp by-products (Peaneus monodon) from Madagascar. J. Food. 9: 220-228. https://doi.org/http://dx.doi.org/ 10.1080/19476337.2010.518250

Rao, Q., A.K. Kamdar & T.P. Labuza. 2016. Storage stability of food protein hydrolysates: A review. Crit. Rev. Food Sci. Nutr. 56: 1169-1193. https://doi.org/10.1080/10408398.2012.758085

Rutherfurd, S.M. 2010. Methodology for determining degree of hydrolysis of proteins in hydrolysates: A review. J. AOAC Int. 93: 1515–1522.

Ruttanapornvareesakul, Y., M. Ikeda, K. Hara, K. Osako, O., Kongpun & Y. Nozaki. 2005. Effect of shrimp head protein hydrolysates on the state of water and denaturation of fish myofibrils during dehydration. Fish. Sci. 71: 220-228.

Seniman, M.S., S.M. Yusop & A.S. Babji. 2014. Production of enzymatic protein hydrolysates from freshwater catfish (Clarias batrachus), in: AIP Conference Proceedings. 323-328. https://doi.org/10.1063/1.4895216

Silva, C.P., R.S. da, Bezerra, A.C.O.dos. Santos, J.B.M.C.R.O.B.de.Castro & L.B.C. Junior. 2017. Biological value of shrimp protein hydrolysate by-product produced by autolysis. Food Sci. Technol. 80. 456-461. https://doi.org/10.1016/j.lwt.2017.03.008

Silva, M.R. 2013. Degree of hydrolysis and peptide profile of whey proteins using pancreatin. Brazilian Soc. Food Nutr. 38: 278-290. https://doi.org/http://dx.doi.org/10.4322/nutrire.2013.026 Degree

Singh, S.M., R.B. Siddhnath, A. Aziz, N. Verma & B.B. Chriwatkar. 2018. Shrimp waste powder: Potential as protein supplement. Int. J. Pure Appl. Biosci. 6. 401-406. https://doi.org/DOI: http://dx.doi.org/10.18782/2320-7051.7141

Sukkhown, P., K. Jangchud & Y. Lorjaroenphon. 2018. Food Hydrocolloids flavored-functional protein hydrolysates from enzymatic hydrolysis of dried squid by-products : Effect of drying method. Food Hydrocoll. 76. 103-112. https://doi.org/10.1016/j.foodhyd.2017.01.026

Thi, H., T. Vy, T.T. Truc & N. Van. Muoi. 2018. Optimization of protein hydrolysis conditions from shrimp head meat (Litopenaeus vannamei) using commercial alcalase and flavourzyme enzymes. Can Tho Univ. J. Sci. 54. 16-25. https://doi.org/10.22144/ctu.jsi.2018.090

Veeranjaneyulu, K., K.C. Dora & B. Koteswar. 2013. Research article a study on recovery of protein hydrolysate from industrial shrimp waste and its nutritional status. Int. J. Curr. Res. 5. 3656-3661.

Walker J.M., 1983. Protein and Peptide Sequence Determination, in: Walker J.M., Gaastra, W. (Eds.), Techniques in Molecular Biology. Springer, Dordrecht., pp. 87-112. https://doi.org/s://doi.org/10.1007/978-94-011-6563-1_5

Wisuthiphaet, N., S. Klinchan & S. Kongruang. 2016. Fish protein hydrolysate production by acid and enzimatic hydrolysis. KMUTNB Int. J. Appl. Sci. Technol. 9: 261-270. https://doi.org/DOI: 10.14416/j.ijast.2016.11.004

Wisuthiphaet, N & S. Kongruang. 2015. Production of Fish protein hydrolysates by acid and enzymatic hydrolysis. J. Med. Bioeng. 4: 466-470. https://doi.org/10.12720/jomb.4.6.466-470



DOI: https://doi.org/10.22146/jfs.59906

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