STUDI AWAL KARAKTERISASI GENETIK ALLOZIM Galaxias truttaceus VALENCIENNES (PISCES : GALAXIIDAE) DI BAGIAN BARAT VICTORIA, AUSTRALIA

https://doi.org/10.22146/jfs.8893

Tuty Arisuryanti(1*)

(1) Laboratory of Genetics, Faculty of Biology, Gadjah Mada University
(*) Corresponding Author

Abstract


The purpose of this study was to determine allozyme genetic characters of the spotted galaxid in western Victoria, Australia. Two populations, Barongarook Creek (landlocked population) and Dutton Creek (diadromous population), of the spotted galaxias (Galaxias truttaceus) was investigated on their genetic characters by allozyme electrophoresis methods. From 23 system enzymes and general protein, 39 putative enzyme-coding loci were resolved, 3 of which were polymorphic at Esterase-1 (Est-1), Glycerol-3-phosphate dehydrogenase-2 (Gpdh-2), and Phosphogluconate hydrogenase (6pgd). Fixed allozyme differences were found at Est-1 locus indicating that incipient change in genetic character have been started in the landlocked population.

Keywords


allozyme electrophoresis, Galaxias truttaceus, genetic characterization

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References

Arisuryanti, T. 1999. Molecular Genetic Variation in Galaxias maculatus (Osteichthyes : Galaxiidae). M.Sc. Thesis Deakin University, Victoria, Australia. 31-47.

Arisuryanti, T. 2000. A preliminary study of genetic variation of Galaxias olidus (Slamoniformes : Galaxiidae) in western Victoria, Australia. Biologi 9 (2) : 487-498.

Austin, C.M. and B. Knott. 1996. Systematic of the freshwater crayfish genus Cherax (Decapoda : Parastacidae) in south western Australia : Electrophoretic, morphological and habitat variation. Australian Journal of Zoology 44 : 223-258.

Carvalho, G. R. 1993. Evolutionary aspects of fish distribution: genetic variability and adaptation. Journal of Fish Biology 43 : 53–73.

Hebert, P.D.N. and Beaton, M.J. 1993. Methodologies for allozyme analysis using cellulose acetate electrophoresis. Department of Zoology, University of Guelph. 32 p.

Humpries, P. 1989. Variation in the life history of diadromous and landlocked populations of the spotted galaxias, Galaxias truttaceus Valenciennes, in Tasmania. Australian Journal of Marine and Freshwater Research 40 : 510-518.

Humpries, P. 1990. Morphological variation in diadromous and landlocked populations of the spotted galaxias, Galaxias truttaceus, in Tasmania, south-eastern Australia. Environmental Biology of Fishes 27 : 97-105.

McDowall, R.M.1996. Freshwater fishes of South-eastern Australia. Reed Books. Australia. 77 p.

McDowall, R.M. and R.S. Frankenberg. 1981. The galaxiid fishes of Australia (Salmoniformes : Galaxiidae). Records of the Australian Museum 33 : 443-605.

Merrick, J.R. and G.E. Schmida. 1984. Australian freshwater fishes : Biology and Management. Griffin Press Limited. Australia. 105 p.

Murphy, R.W., J.W.J. Sites, D.G. Buth, C.H. Haufler. 1996. Proteins : Isozyme Electrophoresis. In : Molecular Systematics. Hillis, D.M., C. Moritz and B.K. Mable. Sinauer Associates, Inc., Sunderland. USA. 51-120.

Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89 : 583-590.

Ovenden, J.R. and R.W.G. White. 1990. Mitochondrial and Allozyme Genetics of Incipient Speciation in a Landlocked Population of Galaxias truttaceus (Pisces: Galaxiidae). Genetics 124(3): 701–716.

Ovenden, J.R., R.W.G. White, and M. Adams. 1993. Mitochondrial and allozyme genetics of two Tasmanian galaxiids (Galaxias auratus and Galaxias tanycephalus (Pisces : Galaxiidae) with restricted lacustrine distributions. Heredity 70 : 223-230.

Richardson, B.J., P.R. Baverstock, and M. Adams. 1986. Allozyme electrophoresis. Academic Press. Australia. 377 p.

Shaklee, J.B. and C.P. Keenan. 1986. A practical laboratory guide to the techniques and methodology of electrophoresis and its application to fish fillet identification. Australian CSIRO Marine Laboratory Rep. : 177.

Swofford, D.L. and M.B. Selander. 1981. BIOSYS-1. A fortran program for the comprehensive analysis of electrophoretic data in population genetics and systematics. Journal of Heredity 72 : 281-283.

Ward, R. D., M. Woodwark and D. O. F. Skibinski. 1994. A comparison of genetic diversity levels in marine, freshwater, and anadromous fishes. Journal of Fish Biology 44 : 213–232.

Watts, R.J., A.W. Storey, D.R. Hebbert and D.H.D. Edward. 1995. Genetic and morphological differences between populations of the Western Minnow, Galaxias occidentalis, from two river systems in south-western Australia. Marine and Freshwater Research 46 : 769-777.



DOI: https://doi.org/10.22146/jfs.8893

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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