Cover Image

Genotyping of rice (Oryza sativa L.) plants according to their root distribution pattern and their tolerance to drought

https://doi.org/10.22146/ipas.84677

Adin Novitasari(1), Indrastuti Apri Rumanti(2), Rina Hapsari Wening(3*), Damanhuri Damanhuri(4)

(1) Faculty of Agriculture, Universitas Brawijaya
(2) Pusat Riset Tanaman Pangan, BRIN
(3) Pusat Standarisasi Instrumen Tanaman Pangan
(4) Faculty of Agriculture, Universitas Brawijaya
(*) Corresponding Author

Abstract


Drought condition is one of the major problems in producing rice in Indonesia. In plant breeding, selection is the main step to create superior varieties. An indicator of drought stress-tolerant rice varieties is the root distribution pattern because it describes the behavior of the roots in searching for water for photosynthesis and metabolism processes. One of the morphological traits related to drought tolerance in rice is root growth and development. This study aimed to determine the morphological and anatomical characteristics of drought-tolerant rice roots and identify drought-tolerant genotypes. The experiments were arranged in a factorial randomized block design with four replications. The first factor was genotype, consisting of ten genotypes. The second factor was drought stress, consisting of two environments without drought and with drought stress. Drought was given only in the vegetative phase, i.e., 1–14 days after planting. WINDEX analysis was performed to determine rice drought tolerance and identify drought-tolerant genotypes. The results showed that three out of the ten tested genotypes had higher WINDEX values, namely BP30411f (7.62), B13983-KA-6-3 (7.99), and BP29790d-PWK-2-SKI-1-3 (9.25). Based on the root distribution pattern, plants with longer primary root lengths, more seminal roots, longer seminal root lengths, and high root angles were predicted to be drought tolerant characteristics. Selection of these characters could be used in future rice breeding programs to obtain plants with superior genotypes.

Keywords


Drought tolerance, meta-xylem, rice, root distribution, WINDEX analysis

Full Text:

PDF


References

Abdullah, A. A., Ammar, M. H., and Badawi, A. T. (2010). Screening rice genotypes for drought resistance in Egypt. Journal of Plant Breeding and Crop Science, 2(7), pp. 205–215.

Anosheh, H.P., Saed‐Moucheshi, A., Pakniyat, H., and Pessarakli, M. (2016). Stomatal Responses to Drought. Water Stress and Crop Plants: A Sustainable Approach. 1st ed. USA: John Wiley & Sons, Ltd., pp, 24–40.

Arve, L.E., Terfa, M.T., Gislerod, H.R., Olsen, J.E., and Torre, S. (2013). High Relative Air Humidity And Continuous Light Reduce Stomata Functionality by Affecting the ABA Regulation in Rose Leaves. Plant Cell Environmental, 36, pp. 382–392.

Banoc, M.D., Yamauchi, A., Kamoshita, A., Wade, L.J., and Pardales, J.R. (2000). Dry matter production and root system development of rice cultivars under fluctuating soil moisture. Plant.Prod.Sci., 3(2), pp. 197–207.

Christopher, J., Christopher, M., Jennings, R., Jones, S., Fletcher, S., and Borrell, A. (2013). QTL for root angle and number in a population developed from bread wheats (Triticum aestivum) with contrasting adaptation to water-limited environments. Theory Appl Genet, 126, pp. 1563–1574.

Comas, L.H., Mueller, K.E., Taylor, L.L., Midford, P.E., Callahan, H.S., and Beerling, D.J. (2012). Evolutionary Patterns and Biogeochemical Significance of Angiosperm Root Traits. International Journal of Plant Science, 173, pp. 584–595.

Fang, Y., and Lizhong, X. (2015). General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular and Molecular Life Sciences, 72(4), pp. 673–689.

Ghosh, D., and Xu, J. (2014). Abiotic stress responses in plant roots: A proteomics perspective. Front Plant Science, 5(6), pp. 1–13.

Henry, A., Cal, A.J., Batoto, T.C., Torres, R.O., and Serraj, R. (2012). Root attributes affecting water uptake of rice (Oryza sativa L,) under drought. Journal Experiment Botany, 63, pp. 4751–4763.

Jafari, S., Garmdareh, S.E.H., and Azadegan, B. (2019). Effects of drought stress on morphological, physiological, and biochemical characteristics of stock plant (Matthiola incana L.). Science Horticultura, 253, pp. 128–133.

Kim, Y.H., Chung, Y.S., Lee, E., Tripathi, P., Heo, S., and Kim, K.H. (2020). Root Response to Drought Stress in Rice (Oryza sativa L.). International Journal Molecular Science, 21(1513), pp. 1–22.

Kurniasih, B., and Wulandhany, F. (2009). Leaf rolling, crown and root growth of several upland rice varieties under different water stress conditions. Agrivita, 31, pp. 118–128.

Limbongan, Y. (2008). Genetic analysis and selection of superior genotypes of lowland rice (Oryza sativa L.) for adaptation to highland ecosystems. Disertation. Institut Pertanian Bogor.

Man, D., Bao, Y.X., and Han, L.B. (2011). Drought tolerance associate with proline and hormone metabolism in two tall fescue cultivars. Horticultura Science, 46(7), pp. 1027–1032.

Nahar, S., Jyotirmay, K., Lingaraj, S., and Bhaben, T. (2016). Morphophysiological and molecular effects of drought stress in rice. Annals of Plant Sciences, 5(9), pp. 1409–1416.

Rangappa, R.B., Jena, K., and Jagadish, K. (2016). Exploring rice and sorghum roots-can root anatomical modification increase drought tolerance in rice?. Phoenix Convention Center North, Room 225 B, Phoenix AZ.

Sanoh, Y., Takai, T., Yoshinaga, S., Nakano, H., Kojima, M., Sakakibara, H., Kondo, M., and Uga, Y. (2014). Deep Rooting Conferred by DEEPER ROOTING 1 enhances rice yield in paddy fields. Scientific Reports, 4, 5553.

Silitonga, S.T. (2004). Management and utilization of rice germplasm in Indonesia. Buletin Plasma Nutfah, 10(2).

Sujinah and Jamil, A. (2016). The Response mechanism of rice plants drought stress and tolerant varieties. Iptek Tanaman Pangan, 11(1).

Taiz, L., and Zeiger, E. (2002). Plant Physiology – Third edition. Massachussetts: Sinaue associaties Inc, 609.

Tubur, H.W., Chozin, M.A., Santosa, E., and Junaedi, A. (2012). Agronomic response of rice varieties to periods of drought in paddy fields. Jurnal Agron, Indonesia, 40(3), pp. 167–173.

Uga, Y., Sugimoto, K., Ogawa, S., Rane, J., Ishitani, M., Hara, N., Kitomi, Y., Inukai, Y., Ono, K., and Kanno, N. (2013). Control of root system architecture by DEEPER ROOTING 1 increases rice yield under drought conditions. Nature Genetics, 45, pp. 1097–1102.

Wasson, A.P., Richards, R.A., Chatrath, R., Misra, S.C., Prasad, S.V., Rebetzke, G.J., Kirkegaard, J.A., Christopher, J., and Watt, M. (2012). Traits and selection strategies to improve root systems and water uptake in water-limited wheat crops. Journal Experiment Botany, 63, pp. 3485–3498.

Wening, R.H. and Untung, S. (2021). Study of rice root distribution patterns for breeding of drought tolerant varieties. IOP Conference Series: Earth and Environmental Science, 911(1), 012013.



DOI: https://doi.org/10.22146/ipas.84677

Article Metrics

Abstract views : 956 | views : 587

Refbacks

  • There are currently no refbacks.


Bookmark and Share



Ilmu Pertanian (Agricultural Science) ISSN 0126-4214 (print), ISSN 2527-7162 (online) is published by Faculty of Agriculture Universitas Gadjah Mada collaboration with Perhimpunan Sarjana Pertanian Indonesia (PISPI) and licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

web analytics View My Stats