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Isolation and Testing of Potato Plant Endophytic Fungi as Inhibitors of Fusarium oxysporum Growth In Vitro
https://doi.org/10.22146/jpti.108020 Susiana Purwantisari(1*), Afni Diva Runnavilla(2), Sri Pujiyanto(3), Siti Nur Jannah(4), Nintya Setiari(5)
(1) Department of Biology, Faculty of Science and Mathematics, Diponegoro University Jln. Prof. Soedarto SH., Semarang, Central Java 50275 Indonesia
(2) Department of Biology, Faculty of Science and Mathematics, Diponegoro University Jln. Prof. Soedarto SH., Semarang, Central Java 50275 Indonesia
(3) Department of Biology, Faculty of Science and Mathematics, Diponegoro University Jln. Prof. Soedarto SH., Semarang, Central Java 50275 Indonesia
(4) Department of Biology, Faculty of Science and Mathematics, Diponegoro University Jln. Prof. Soedarto SH., Semarang, Central Java 50275 Indonesia
(5) Department of Biology, Faculty of Science and Mathematics, Diponegoro University Jln. Prof. Soedarto SH., Semarang, Central Java 50275 Indonesia
(*) Corresponding Author
Abstract
Potato (Solanum tuberosum) crop failure is a common problem for farmers. One of the main causes is Fusarium disease, caused by Fusarium oxysporum. One method to inhibit the pathogen is to use endophytic fungi from potato plants. The purpose of this study was to isolate endophytic fungi from potato plants and to test their potential as inhibitors of F. oxysporum growth. The research included isolation, morphology identification, pathogenity test, and antagonism assay of the endophytic fungi. The experiment used a Completely Randomized Design (CRD). The results of the pathogenicity test showed that F. oxysporum could infect potato plants by showing fusarium wilt symptoms. Isolation results potato plants endophytic fungi obtained eight isolates, which five isolates were from the genus Aspergillus, two isolates from the genus Penicillium, and one isolate from the genus Mucor. The results of the antagonism test showed that the eight isolates were antagonistic against F. oxysporum. Six isolates showed high inhibition (60–77%), while two isolates showed moderate inhibition (43–58%). Eight isolates showed antagonistic mechanisms by competiting for space and nutrients as well as showing antibiosis activity.
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References
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DOI: https://doi.org/10.22146/jpti.108020
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