* Corresponding Author
Production of N-acetylglucosamine from Semi Purified Chitinase of Mucor circinelloides that Immobilized by using Agar
https://doi.org/10.22146/jfs.45886 Lucia Crysanthy Soedirga(1*), Hardoko Hardoko(2), Natasha Vania Widianto(3)
(1) Program Studi Teknologi Pangan, Fakultas Sains dan Teknologi Universitas Pelita Harapan, Karawaci-Tangerang
(2) Program Studi Teknologi Pangan, Fakultas Sains dan Teknologi Universitas Pelita Harapan, Karawaci-Tangerang
(3) Program Studi Teknologi Pangan, Fakultas Sains dan Teknologi Universitas Pelita Harapan, Karawaci-Tangerang
(*) Corresponding Author
Abstract
Hydrolysis of chitin into N-acetylglucosamine (NAG) can be done enzymatically by using chitinase enzyme that obtained from the fermentation with chitinolytic molds. Mucor circinelloides is one example of chitinolytic mold that can produce semi purified chitinase enzyme during the fermentation of chitin from black tiger shrimp (Penaeus monodon) shell. The semi purified chitinase enzyme that obtained in this research will be immobilized into the agar polymer to the extent of its stability during NAG production. This research was aimed to investigated the best concentration of agar (3,4,5, and 6%) and the best amount of added enzyme (0.2; 0.4; 0.6; 0.8; and 1 mL) toward the production of NAG. The result showed 0.6 ml of semi purified chitinase that immobilized into 3% of agar can produced NAG within the concentration 1111.667 ppm. Moreover, this enzyme considerably stable before and after immobilization within the value of 4.78 U/mL.
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Agustina, S., I.M.D. Swantara & I.N. Suartha. 2015. Isolasi kitin, karakterisasi, dan sintesis kitosan dari kulit udang. Jurnal Kimia 9. (2): 271-278
Badan Standarisasi Nasional [BSN]. 2013. SNI 7948:2013. “Kitin: Syarat Mutu dan Pengolahan.” Badan Standarisasi Nasional, Jakarta
Febrianto, R. 2018. Optimum condition for N-Acetylglucosamine production from tiger shrimp (Penaus monodon) shell using extracellular crude chirinase enzyme from Mucor circinelloides. Skripsi, Universitas Pelita Harapan, Tangerang
Hamed, I., F. Özogul & J.M. Regenstein. 2016. Industrial applications of crustacean by-products (chitin, chitosan, and chitooligosaccharides): A review. Trends in Food Science & Technology. 48: 40-50
Herowati, R. 2014. Obat dan suplemen untuk osteoarthritis. Jurnal Pharmacy. 11 (1): 40-48
Ishmayana, S., Alfitri, D. Sadiah, D.R. Saadah & S. Agus. 2012. Kinerja fermentasi ragi Saccharomyces cerevisiae pada media VHG dengan variasi konsentrasi ekstrak ragi sebagai sumber nitrogen untuk produksi bioetanol. Seminar Nasional Kimia dan Pendidikan Kimia (312-317)
Jenifer, S., J. Jeyasree, Kezia, D. Laveena & K. Manikandan. 2014. Purification and characterization of chininase from Tricodherma viride N9 and its antifungal against phytopathogenic fungi. World Journal of Pharmacy Sciences. 3 (12): 1604-1611
Kementrian Kelautan dan Perikanan (KKP). 2017. MEA Centre. Sektor Kelautan dan Perikanan
Li, J., F. Yao, Y. Yin & K. De Yao. 2016. From chitin to chitosan. In Chitosan-Based Hydrogels. CRC Press. 7-43
Minteer, S.D. 2017. Enzyme Stabilization and Immobilization. New York, NY: Springer New York
Prakash, J., R.K. Gupta, X.X. Priyanka & V.C. Kalia. 2018. Bioprocessing of biodiesel industry effluent by immobilized bacteria to produce value-added products. Applied Biochemistry and Biotechnology. 185 (1): 179-190
Prakash, O.N & Jaiswal. 2011. Immobilization of a thermostable α-Amylase on agarose and agar matrices and its application in starch stain removal. World Applied Science Journal. 13: 572–577
Rahmansyah, M., & Sudiana, M. 2003. Optimasi analisis amilase dan glukanase yang diekstrak dari miselium Pleurotus ostreatus dengan asam 3,5 dinitrosalisilat. Jurnal Penelitian Hayati 9 (1) : 7-12
Teja, E. 2018. Optimasi Produksi N-Asetilglukosamin dari Kulit Udang Windu dengan Menggunakan Enzim Kitinase Intraseluler Semi Murni Providencia stuartii. Skripsi. Universitas Pelita Harapan, Tangerang
Thirumavalavan, M & J.F. Lee. 2015. A short review on chitosan membrane for biomolecules immobilization. Journal of Molecular and Genetic Medicine. 9 (178): 1747-0862
Veronica. 2018. Isolasi dan Identifikasi Kapang Penghidrolisis Kitin yang Diisolasi dari Kitin Kulit Udang Windu (Penaus monodon). Skripsi, Universitas Pelita Harapan
Waltam, D.R. 2009. Demineralisasi dan Deproteinasi Kulit Udang Secara Kontinyu pada Tahapan Ekstraksi Kitin Secara Biologis. Skripsi, Universitas Indonesia, Depok
Younes, I & Rinaudo. 2015. Chitin and chitosan preparation from marine sources. Structure, Properties and Application. Marine Drugs. 13: 1133-1174
Zdarta, J., L. Klapiszewski, M. Wysokowski, M. Norman, A, Kołodziejczak-Radzimska, D. Moszyński & T. Jesionowski. 2015. Chitin-lignin material as a novel matrix for enzyme immobilization. Marine Drugs. 13 (4): 2424-2446 DOI: https://doi.org/10.22146/jfs.45886
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