Antimicrobial Substance Produced by Lactobacillus sp. TGR-2 Isolated From Growol

https://doi.org/10.22146/jifnp.47

Titiek F. Djaafaru(1*), Endang S. Rahayu(2), Djoko Wibowo(3), Slamet Sudarmadji(4)

(1) Instalation for Research and Assessment of Agricultural Technology
(2) Gadjah Mada University
(3) Gadjah Mada University
(4) Gadjah Mada University
(*) Corresponding Author

Abstract


Lactic acid bacteria strain TGR-2 was isolated from growol (fermented raw cassava) and then identified as Lactobacillus sp. TGR-2. According to results of the turbidimetric assay, the neutralized supernatant of Lac¬tobacillus sp. TGR-2 were able to inhibit the growth of spoilage and pathogenic bacteria, i.e., Staphylococcus aureus FNCC 0047, Salmonella iyphimurium FNCC 0050, Escherichia coli FNCC 0091, Bacillus cereus FNCC 0057, and Morganella morganii FNCC 0122. The antimicrobial activity of neutralized supernatant of Lactobacillus sp. TGR-2 was stable at room tempera¬ture for 60 min, pH 3-8; heating 98 °C for 30 min, pH 3-8; 121 °C for 15 min, pH 3-8; storage at 4 °C for 21 days, p1-1 6.5. The third fraction obtained from purified of supernatant of Lactobacillus sp. TGR-2 by gel filtra¬tion which possessed the molecular weight 14,000 Dalton has a bactericidal effect on the growth of S. aureus.

Full Text:

PDF


References

Atrich, A., Rekhif, N., Milliere, J. B., and Lefebvre, G. 1993. Detection and characterization of a bacteriocon produced by Lactobacillus plantarum C19. Canadian Journal of Microbioogy. 39: 1173-1179

Abdel-Bar, N., Harris, N. D., and Rill, R, L. 1987. Purification and properties of an antimicrobial substance produced by Lactobacillus bulgaricus. Journal of Food Science. 52: 411-415

Bhunia, A. K., Johnson, M. C., and Ray, B. 1987. Direct detection of an antimicrobial peptide of Pedisoccus acidilactici in sodium dedocyl sulfate-polyacrylamid gel electrophoresis. Journal of Industrial Microbiology. 2: 319-322

Bhunia, A. K., Johnson, M. C., and, Ray, B. 1988. Purification, characterization and microbial spectrum of a bacteriocin produced by Pediococcus acidilactici. J. Applied Bacteriology. 65: 261-268

Branen, A. L., Go, H. C., and Genske, R. P. 1975. Purification and properties of antimicrobial substances produced by Streptococcus diacetilactis and Leuconostoc citrovorum. Journal of Food Science. 40: 446-450

Cintas, L. M., Rodriguez, J. M., Fernandez, M. F., Sletten, K., Nes, I. F., Hernandez, P. E., and Holo, H. 1995. Isolation and characterization of pediocin L50, a new bacteroicin from Pediococcus acidilactici with broad inhibitory spectrum. Applied and Enviromental Microbiology. 61: 2643-2648

Daeschel, M. A. 1989. Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food Technology. 43: 164-167

Eckner, K. F. 1992. Bacteoricin and food applications. Dairy, Food and Environmental Saturation. 4: 204-209

Kojic, M., Svircevic, J., Banina, A., and Topisirovic, L. 1991. Bacteroicin-producing strain of Lactococccus lactis subsp. diacetilactis S50. Applied Environmental Microbiology. 57: 1835-1837

Lewus, C. B., Kaieser, A. and Montville, T. J. 1991. Inhibition of food-borne bacteria isolated from meat. Applied Environmental Microbiology. 57: 1683-1688

Muriana, P. M. and Luchansky, J. B. 1993. Biochemical methods for purification bacteriocin. In "Bacteriocin of lactic acid bacteria" Ed. D.G. Hoover and L. R. Steenson, pp. 41-61. Academic Press, Inc. New York.

Schilinger, U. and Holzapfel, W. H. 1990. Antibacterial activity of carnobacteria. Food Microbiology. 7: 305-310

Schilinger, U. nd Lucke, F. K. 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Applied Environmental Microbiology. 55: 1901-1906.

Scopes, R. K. 1987. "Protein purification: Principles and practice". Springer, Verlag.

Spelhaug, S. R. and Harlander, S. K. 1989. Inhibtion of food-borne bacterial pathogens by bacteriocins from Lactococcus lactis and Pediococcus pentosaceus. Journal of Food Protection. 52: 856-862

Stamer, J. R. 1979. The lactic acid bacteria: Microbes of diversity. Food Technology. 1: 60-65

Stoffles, G., Nissen-Meyer, J., Gudmundsottir, A., Sletten, K., Holo, H., and Nes, I. F. 1992. purification and characterization of new bacteriocin isolated from a Carnobacterium sp. Applied and Environmental Microbiology. 58: 1417-1422



DOI: https://doi.org/10.22146/jifnp.47

Article Metrics

Abstract views : 2647 | views : 1551

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Food and Nutrition Progress (print ISSN 0854-6177; online ISSN 2597-9388) is published by the Indonesian Association of Food Technologists in collaboration with the Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

Indexed by: