Mutation Technique for Increasing the Production of Antibacteria Lactobacillus plantarum TGR-2

https://doi.org/10.22146/jifnp.71

Sebastian Margino(1*), Sri Winarti(2), Retno Indrati(3), Endang S. Rahayu(4)

(1) Gadjah Mada University
(2) Gadjah Mada University
(3) Gadjah Mada University
(4) Gadjah Mada University
(*) Corresponding Author

Abstract


Production of antibacteria of Lactobacillus plantarum TGR-2 was carried out by mutation technique using UV radiation and chemical mutagenic agent (acrydine orange (AO), ethyl methane sulfonate (EMS), and N-methyl-N-nitro sulfonate nitrosoguanidine (NTG). As a marker was applied chloramphenicol for 10 ppm. Total 214 mutants were obtained from all of the treatments and then screened based on their activities
against Staphylococcus aureus FNCC 0047 as
bacterial indicator, using agar diffusion and turbidimetric assay techniques. Results showed that 24 mutants could produce higher amount of antibacteria and two of them, NTG and acrydine orange treatments, had higher stability than others. Characterization of two mutants, determination of biochemistry traits and optimization of antibacterial (bacteriocin) production were done. The product showed that UV-15, EMS-10, AO-6 and NTG-21 mutants could produce antibacterial substance 3,90, 4.40, 3.40 and 4.17 AU/ml for 16, 12, 12, and 16 hours incubation, respectively, compared to Lactobacillus plantarum TGR-2 which produced 1.07 UA/ ml for 24 hours incubation.

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References

Adams, R.L.P., 1986. The biochemistry of the nucleic acids. Chapman and Hall, University Press, Cambridge.

Angus, B.L., J.A.M. Fyte and R.E.W. Hancock, 1987. Mapping and characterizing of two antibiotic supersusceptibility in Pseudomonas aeroginosa. J. Gen Micro. 133: 2905–2914.

Chasy, B.M. 1987. Prospect for genetic manipulation of lactobacilli. FEMS Microbiol. Rev. 46: 297–312.

Daeschel, M.A., 1989. Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food Technology. 43: 164–167.

Djaafar, T.F., E.S. Rahayu, D. Wibowo, and S. Sudarmadji. 1996. Antimicrobial substance produced by Lactobacillus sp. TGR-2 isolated from growol. Indonesian Food and Nutrition Progress 3 (2): 29-34.

Eckner, K.F. 1992. Bacteriocin and food applications. Dairy, Food and Environmental Sanitation. 4 : 204-209.

Eisenstadt, E., B.C. Carlton and B.J. Brown. 1994. Gene Mutation. In : Methods for General and Molecular Bacteriology (ed. P. Gerhardt) American Society for Microbiology, Washington, D.C.

Franklin, T.J. and G.A. Snow. 1989. Biochemistry of Antimicrobial Action. Fourth edition. Chapman and Hall. New York.

Gichner, T. and J. Veleminsky. 1988. Mechanism of inhibition of N-nitroso compounds-induced mutagenicity. Mutation Research. 202 : 205-211.

Gilliland, S.E. 1985. Bacterial Starter Cultures for Foods. CRC Press, Inc. Boca Raton, Fla.

Holzapfel, W.H. and B.J.B. Wood. 1995. Lactic acid bacteria in contemporary perspective. In : The Genera of Lactic Acid Bacteria (Ed B.J.B. Wood and W.H. Holzapfel), Blackie Academic and Professional, London.

Rahayu, E.S., T.F. Djaafar, D. Wibowo, S. Sudarmadji. 1996. Lactic acid bacteria from indigenous fermented foods and their antimicrobial activity. Indonesian Food and Nutrition Progress 3 (2) : 21-28.

Spelhaug, S.R. and S.K. Harlander. 1989. Inhibition of foodborne bacterial pathogens by bacteriocin from Lactococcus lactis and Pediococcus pentosaceus. J. Food Protect. 52 (12) 856-862.



DOI: https://doi.org/10.22146/jifnp.71

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Indonesian Food and Nutrition Progress (print ISSN 0854-6177; online ISSN 2597-9388) is published by the Indonesian Association of Food Technologists in collaboration with the Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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