Sinbiotik Ekstrak Ubi Ungu dan Probiotik Lokal pada Yogurt: Kesehatan Pencernaan, Hematologi, dan Sistem Imun

https://doi.org/10.22146/agritech.29718

Agustina Intan Niken Tari(1*), Catur Budi Handayani(2), Sri Hartati(3)

(1) Program Studi Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Veteran Bangun Nusantara, Jl. Letjens S. Humardani, Jombor No. 1 Sukokarjo 57521
(2) Program Studi Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Veteran Bangun Nusantara, Jl. Letjens S. Humardani, Jombor No. 1 Sukokarjo 57521
(3) Program Studi Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Veteran Bangun Nusantara, Jl. Letjens S. Humardani, Jombor No. 1 Sukokarjo 57521
(*) Corresponding Author

Abstract


The bacteria presence in the gastrointestinal tract is one of the important factors determining the support for body health among others. Therefore, it is essential to maintain a population balance, possibly achieved by consuming local probiotic-based sinbiotic yoghurt. The purpose of this study, therefore, was to evaluate the effectiveness of purple sweet potato extracts and local probiotics supplemented in yogurt by a healthy profile. Furthermore, the Health parameters evaluated include gastrointestinal (faecal water), hematology (number of erythosit, leukocyte, hemoglobin and hemtocrit) and immune system (antibody titer) in Spraque dawley albino mice. This study was performed using a Completely Randomized Design on 30 rats divided into 6 groups.  Therefore, those in group K- were administered distilled water from day 1st -21st, YTP =  yogurt without probiotics from day 1st -21st, YDP = probiotic yogurt from day  1st- 21st,  YTP+E = yogurt without probiotic from day 1st- 21st, interspersed EPEC on day 8th – 14th, YDP+E = yogurt from day1st- 21st, interspersed EPEC on day  8th-14th, K + = water from day 1st- 21persed EPEC on day 8th – 14th, YDP+E = probiotic yogurt from day1st- 21st, then fed EPEC on day 8th- 14th. The results showed the effectiveness of probiotic yogurt treatment with purple sweet potato extract supplementation in healthy gastrointestinal (faecal water), hematology (number of erythrocyte, leukocyte, hemoglobin and hematocrit) and immune system (titre antibodies). This phenomenon results from infection by EPEC (Enteropathogenic Eshericchia coli), which is responsible for diarrhea. Furthermore, the YDP treatment possessed a water content characterized by 48.42% feces, while the number of erythrocytes, leucocytes, haemoglobin and hematocrit were 8,58x106/μl, 14,15 x106/μl, 13.98 g/dL and 47,67 mL%, respectively. Moreover, 6,42 log of antibody titers was also determined.

Keywords


Digestive; hematology; immune; synbiotik; yogurt

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References

Aboderin, F. I., & Oyetayo, V. O. (2006). Haematological studies of rats fed different doses of probiotic, Lactobacillus plantarum, isolated from fermenting corn slurry. Pakistan Journal of Nutrition, 5 (2), 102–105. https://doi.org/10.3923/pjn.2006.102.105

Adib, A., H. Wahid, M., Sudarmono, P., & S. Surono, I. (2013). Lactobacillus plantarum pada Feses Individu Sehat yang Mengkonsumsi Lactobacillus plantarum IS-10506 dari Dadih. Jurnal Teknologi Dan Industri Pangan, 24(2), 154–161. https://doi.org/10.6066/jtip.2013.24.2.154

Astawan, M., Wresdiyati, T., Arief, I. I., & Suhesti, E. (2011). Gambaran hematologi tikus putih (Rattus norvegicus) yang diinfeksi Escherichia coli enteropatogenik dan diberikan probiotik. Media Peternakan, 34(1), 7–13. https://doi.org/10.5398/medpet.2011.34.1.7

Douglas JW, Wardrop KJ. (2010). Schalm’s Veterinary Hematology. 6th ed. Wiley-Blackwell. P 852-887.

Dwyana, Z. (2017). Aktivitas Imunoglobin Mencit Jantan Pasca Pemberian Isolat Probiotik Dari Susu Kerbau. Jurnal Ilmu Alam Dan Lingkungan, 8(2). https://doi.org/10.20956/jal.v8i16.2986

Haryono, R. (2012). Keperawatan medikal bedah sistem pencernaan. Yogyakarta: Gosyen Publishing

Jacobi, C. A., Schulz, C., & Malfertheiner, P. (2011). Treating critically ill patients with probiotics: Beneficial or dangerous? Gut Pathogens, 3(1), 2. https://doi.org/10.1186/1757-4749-3-2

Jepi, 2011.Uji Efek Isolat Bakteri Probiotik dari Susu Kerbau Terhadap Aktivitas Imunoglobulin G (IgG) Mencit (Mus musculus) Jantan. Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Hasanuddin. Makasar

Lorenzo, J. M., Munekata, P. E., Dominguez, R., Pateiro, M., Saraiva, J. A., & Franco, D. (2018). Main groups of microorganisms of relevance for food safety and stability: General aspects and overall description. In Innovative technologies for food preservation: Inactivation of spoilage and pathogenic microorganisms. https://doi.org/10.1016/B978-0-12-811031-7.00003-0

Nuraida, L., Palupi, N. S., Bastomi, R. R., Priscilia, D., & Nurjanah, S. (2012). Evaluation of Probiotics Properties of Lactic Acid Bacteria Isolated From Breast Milk and Their Potency As Starter Culture. International Journal of Food, Nutrition and Public Health, 5(1), 33–60. Retrieved from http://www.worldsustainable.org

Nurliyani, ., Julia, M., Harmayani, E., Ikawati, M., & Baliarti, E. (2015). Potency of Lactobacillus plantarum Dad-13 and Sweet Potato (Ipomoea batatas) Fiber as Immunomodulator in Rats Infected With Salmonella Typhimurium. Journal of Food Research, 4(3), 1. https://doi.org/10.5539/jfr.v4n3p1

Oppegård, C., Kjos, M., Veening, J. W., Nissen-Meyer, J., & Kristensen, T. (2016). A putative amino acid transporter determines sensitivity to the two-peptide bacteriocin plantaricin JK. MicrobiologyOpen, 5(4), 700–708. https://doi.org/10.1002/mbo3.363

Rahayu, E.S., Yogeswara, A., Utami, T., Suparmo. (2011). Indigenous Probiotic Strains of Indonesia and their application for fermented food. The 12th Asean food Conference. BITEC Bangn, Bangkok, Thailand 16-18 June 2011.

Rahayu, E. S., Yogeswara, A., Mariyatun, Windiarti, L., Utami, T., & Watanabe, K. (2016). Molecular characteristics of indigenous probiotic strains from Indonesia. International Journal of Probiotics and Prebiotics, 11(2), 109–116.

Riswanto. (2013). Pemeriksaan Laboratorium Hematologi. Alfamedia. Yogyakarta

Sarwono, J dan Hendra Nur Salim. (2017). Prosedur-prosedur Populer Statistik untuk Analisis Data Riset Skripsi. Gaya Media. Yogyakarta

S, Sukarni., & Sukmono, T. (2019). Kajian Penggunaan Ciprofloxacin terhadap Histologi Insang dan Hati Ikan Botia (Botia macracanthus, Bleeker) yang Diinfeksi Bakteri Aeromonas hydrophila. Biospecies, 12(1), 55–67. https://doi.org/10.22437/biospecies.v12i1.6597

Spehlmann, M. E., Dann, S. M., Hruz, P., Hanson, E., McCole, D. F., & Eckmann, L. (2009). CXCR2-Dependent Mucosal Neutrophil Influx Protects against Colitis-Associated Diarrhea Caused by an Attaching/Effacing Lesion-Forming Bacterial Pathogen. The Journal of Immunology, 183(5), 3332–3343. https://doi.org/10.4049/jimmunol.0900600

Sumaryati, B. T., Utami, T., & Suparmo. (2009). Pengaruh Infeksi Escherichia coli dan Pemberian Lactobacillus plantarum Dad 13 terhadap Mikrobiota Feses Tikus Wistar. Agritech, 29(4), 165–170. https://doi.org/https://doi.org/10.22146/

Tari, A. I.N., Catur Budi Handayani dan Ali Mursyid Wahyu Muyono. (2013). Bakteri Probiotik terhadap Mutu Yogurt Ekstrak Ubi Jalar Ungu dan Pemanfaatannya sebagai Pencegah Diare dan Imunomodulator. Laporan Penelitian Hibah Bersaing. Universitas Veteran Bangun Nusantara Sukoharjo.

Tari, A.I.N, Catur Budi Handayani, S. (2016). Potensi Probiotik Indigenous Lactobacillus plantarum Dad 13 pada Yogurt dengan Suplementasi Ekstrak Ubi Jalar Ungu untuk Penurun Diare. Agritech, 36(1), 7–14. https://doi.org/https://doi.org/10.22146/agritech.10677

Tari, A.I.N, Catur Budi Handayani dan Sudarmi. (2017). Probiotik Lokal untuk Kesehatan Saluran Pencernaan, Sistem Kekebalan Tubuh pada Minuman Fermentasi Laktat. Laporan Penelitian Produk Terapan. Universitas Veteran Bangun Nusantara Sukoharjo.

Utami, T., Djaafar, T. F., & Rahayu, E. S. (2014). Growth of Lactobacillus paracasei SNP-2 in Peanut Milk and Its Survival in Fermented Peanut Milk Drink During Storage. Indonesian Food and Nutrition Progress, 13(1), 11–16. https://doi.org/https://doi.org/10.22146/jifnp.116

Varma, P., Dinesh, K. R., Menon, K. K., & Biswas, R. (2010). Lactobacillus Fermentum Isolated from Human Colonic Mucosal Biopsy Inhibits the Growth and Adhesion of Enteric and Foodborne Pathogens. Journal of Food Science, 75 (9). https://doi.org/10.1111/j.1750-3841.2010.01818.x

World Health Organization (WHO). (2010). Pakistan: IDP hosting and crisis affected districts, Khyber, Pakhtunkhwa, week 21, 22-28 May 2010. WHO: Weekly Morbidity and Mortality Report.

Walsham, A. D. S., MacKenzie, D. A., Cook, V., Wemyss-Holden, S., Hews, C. L., Juge, N., & Schüller, S. (2016). Lactobacillus reuteri inhibition of enteropathogenic Escherichia coli adherence to human intestinal epithelium. Frontiers in Microbiology, 7(MAR), 1–10. https://doi.org/10.3389/fmicb.2016.00244

Zhou, Y. K., Qin, H. L., Zhang, M., Shen, T. Y., Chen, H. Q., Ma, Y. L., Liu, Z. H. (2012). Effects of lactobacillus plantarum on gut barrier function in experimental obstructive jaundice. World Journal of Gastroenterology, 18(30), 3977–3991. https://doi.org/10.3748/wjg.v18.i30.3977



DOI: https://doi.org/10.22146/agritech.29718

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