* Corresponding Author
Skrining pada Berbagai Jenis Umbi dan Pisang sebagai Sumber Difructose Anhyride III (DFA III) Melalui Reaksi Enzimatis
https://doi.org/10.22146/agritech.68463 Diah Ratnaningrum(1*), Een Sri Endah(2), Sabarni Gaffar(3), Sri Pudjiraharti(4)
(1) Pusat Riset Lingkungan dan Teknologi Bersih-Badan Riset Inovasi dan Nasional (BRIN), Jl. Cisitu Sangkuriang, Bandung, Jawa Barat 40135
(2) Pusat Riset Lingkungan dan Teknologi Bersih-Badan Riset Inovasi dan Nasional (BRIN), Jl. Cisitu Sangkuriang, Bandung, Jawa Barat 40135
(3) Program Studi Diploma Analis Kimia, Fakultas Matematika dan Ilmu Pengetahuan Indonesia, Universitas Padjajaran, Kampus Jatinangor, Jl. Jatinangor, Sumedang 45360
(4) Pusat Riset Lingkungan dan Teknologi Bersih-Badan Riset Inovasi dan Nasional (BRIN), Jl. Cisitu Sangkuriang, Bandung, Jawa Barat 40135
(*) Corresponding Author
Abstract
Difructosa Anhyride III (DFA III) dapat diproduksi melalui reaksi enzimatis menggunakan inulin. Inulin dapat diperoleh dari akar/umbi dan batang tanaman. Pada penelitian ini, jenis umbi-umbian dan pisang yang diketahui sebagai salah satu sumber inulin dan akan diproses untuk pembuatan DFA III menggunakan inulin fructotransferase (IFTase) Nonomurae sp. Tujuan penelitian ini adalah melakukan tahapan skrining untuk memperoleh bahan baku sumber inulin dari berbagai jenis jenis umbi dan pisang yang berpotensi sebagai sumber pembuatan DFA III. Metode yang digunakan yaitu melalui reaksi enzimatis menggunakan Inulin fruktotransferase (IFTase). Umbi dan pisang diblansing, dipotong dan diblender dan ditambahkan air panas dengan rasio 1:2. Ekstrak inulin yang dihasilkan diukur secara kualitatif menggunakan metoda kromatografi lapis tipis (KLT) dan metode spektrofotometer. Hasil penelitian menujukkan bahwa umbi jalar mempunyai potensi besar dalam pembentukan DFA III dengan kadar inulin sebesar 7.03%, sedangkan untuk jenis pisang tidak berpotensi dalam pembentukan DFA III, hanya memiliki kandungan inulin saja (20,1%).
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Akram, V. & Navneet, G. (2020). Optimization of inulin production process parameters using response surface methodology. Akram and Garud Future Journal of Pharmaceutical Sciences, 6, 68. https://doi.org/10.1186/s43094-020-00087-1
Anonim (2020). Manfaat Umbi ungu untuk Kesehatan. http://blog.sayuranbox.com/7-manfaat-ubi-ungu-untuk-kesehatan/
Apolinario, A. C. (2017). Extraction, isolation and characterization of inulin from Agave bisalana boles. Ind Crops Prod. Vol 108. pp. 355-362.
Avianty & Fitriyono, A. (2018). Indeks glikemik snack bar ubi jalar kedelai hitam sebagai alternatif makanan selingan penderita diabetes melitus tipe 2. Jurnal Aplikasi Teknologi Pangan, 3(3).
Budiwati, T. A., Diah, R., & Sri, P. (2017). Enzymatic of DFA III from fersh dahlia tubers as raw material. International Symposium on Applied Chemistry (ISAC). AIP Confrence. Proc. 1803, 0200031-1-02003.
Crespo I., Augusto, A., Jessica, T. L., Phoebe, A. R., Agus, B. N. P. & Ritchie, R. (2020). Inulin from several tubers available in Indonesia and the growth of gut microbiota. Indonesian Journal of Life Sciences, 2(1). https://doi.org/10.54250/ijls.v2i1.33
Chi, Z. Chi, T. Zhang, G. Liu, L., & Xue (2009). Inulinase-expressing microorganisms and applications of inulinases. Appl. Microbiol. Biotechnol, 82, 211–220.
Christianingsih, R. & Ivana, T. D. (2015). Kajian kandungan mineral dan asam sianida umbi gadung (Discorea hispida Denn st) pada berbagai Umur Panen. Agro UPV, 6(2). ISSN 1978-2276.
De Bruyn, A., Alvarez, A. P., Sandra, P., & DeLeenheer, L. (1992). Isolation and identification of O-b-D-fructofuranosyl-(2à1)-O-b-D-fructosuranosyl-(2à1)-D-fructose: a product of the enzymic hydrolysis of the inulin from Cichorium intybus. Carbo-hydrate Res, 167992, 235:303
Diesche, Z. & Borenfreund, E. (1951). A new spectrofotometric method for detection and determination of keto sugars and trioses. The Journal of Biological Chemistry, 192(2), 83-587.
Endah, E. S., Meli, F., Rini, M., Diah, R., Puspita, L., & Sri, P. (2019). Optimization of medium composition for inulin fructotransferase (IFTase) production by Nonomuraea sp. IOP Conf. Series: Earth and Environmental Science, 439 (1) 012043. http://doi.10.1088/1755-1315/439/1/012043
Fadjriani, Fadly, Y., Tantu, & Asriani, H. (2018). Kajian penggunaan umbi bunga dahlia (Dahlia viriabilis) dalam produksi kerupuk ikan lele (Clarias batruchus) fungsional. Mitra Sains, 6(1), 61-71. ISSN 2302-2027
Glinsman, W., Kritchevsky, D., Prosky, L. & Roberfroid, M. (2001). Inulin and oligofructose as dietary fiber: a review of the evidence. Critical Reviews in Food Science and Nutrition, 41(5), 353–362.
Gao, H., Shaobo, Huang, Tao, D., Qiaosong, Y., & Ganjun, Y. (2016). Analysis of resistant starch degradation in postharvest ripening of two banana cultivars: Focus on starch structure and amylases. Postharvest Biology and Technology, 119.
Haraguchi, K. (2011). Two types of inulin fructotransferases. Materials 4, 1543-1547; http://doi:10.3390/ma4091543
Hiroshi, H., Onoshima, S., & Nakagawa. (2010). Difructose anhydride III promotes iron absorption in the rat large intestine. Chie Citation Nutrition, 26(1), 120-127. https://doi.org/10.1016/j.nut.2009.05.02
Hilman, A., Harmayani, E., & Cahyanto, M. N. (2018). Inulin extraction and characterization of fresh and chip gembili (Dioscoreae esculenta) extract by ultrasound-assisted extraction. In Proceeding of the Iternational Conference of Science, Technology Engineering. Environmental and Ramilication Resercher (ICOSTEER)-Researcher in Industry 4.0, pages 47-53. DOI: 10.5220/0010084000470053
Harepa, W. & Usman, P. (2017). Evaluasi tingkat kematangan buah terhadap mutu tepung pisang kepok yang dihasilkan. Jom Paferta, 4(2), 1-12.
Indarto & Murinto (2017). Deteksi kematangan buah pisang berdasarkan fitur warna citra kulit pisang menggunakan metode transformasi ruang warna HIS. Juita, 5(1).
Kaur, N. & Gupta, A. K. (2002). Applications of inulin oligofructose in health and nutrition. Journal of Biosciences 27, 703-714.
Kikuchi, H. (2009). Industrial production of difructose anhydride III (DFA III) from crude inulin extracted from chicory roots using Arthrobacter sp. H65-7 fructosyl transferase. J. Biosci Bioeng 107, 262–265
Kierstan, M. P. K. J. (1980). Production of Syrup From Inulin, Process Biochem.
Kosasih, W., S, Pudjiraharti, Diah, R. & Sri, P. (2015). Preparation of inulin from dahlia tubers. International Symposium on Applied Chemistry. Procedia Chemistry 16, 190–194.
Minamida, K., Kaneko, M., Ohashi, M., Sujaya, I. N., Sone T., Wada M., Yokota A., Asano, K., & Tomita, F. (2005). Effects of difructose anhydride III (DFA III) administration on bile acids and growth of DFA III-Assimilating bacterium Ruminococcus productus on rat intestine. J. Biosci. Bioeng 99, 548–554.
Mitamura, R., Hara, H., Aoyama, Y. & Chiji, H. (2002). Supplemental feeding of difructose anhydride III restores calcium absorption impaired by ovariectomy in rats. J Nutr 132, 3387–3393
Minamida, K., Shiga, K., Sujaya, I. N., Sone, T., Yokota, A., Hara, H., Asano, K., & Tomita, F. (2005). Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota. Journal of Bioscience and Bioengineering, 99(3), 230-236. https://doi.org/10.1263/jbb.99.230
Nines, K. R. (1999). Inulin and Oligofructose: What Are They? Journal of Nutrition. Suppl., 129, 1402S-14604S.
Petkove, N. T., Sherova, G., & Denev, P. P. (2018). Characterization of inulin from dahlia tubers isolated by microwave and ultrasound - assisted extraction. International Food Research Journal, 25(5), 1876-1884.
Pudjiraharti, S., Dasli, N., Diah, R., & Endah, E. S. (2016). Preparation od DFA III From Dahlia (Dahlia sp) Tubers by Nonomurae sp. Inulin Fructotransferase. Teknologi Indonesia, 39(1), 22-28.
Pudjiraharti, S., Ohtani, M., Takano, N., Abe, A., Lisdiyanti, P., Tanaka, M., Sone T., & Asano, K. (2014). Nonomuraea sp. ID06-A0189 inulin fructotransferase (DFA III-forming): gene cloning, characterization and conservation among other Nonomuraea species. The Journal of Antibiotics, 67, 137–141.
Pudjiraharti, S. (2015). Pengembangan Produk Nutrasetikal Kombinasi DFA III - Ekstrak Jahe untuk Osteoporosis. Laporan Kegiatan Unggulan LIPI.
Pudjiraharti, S., Takesue, N., Katayama, T., Lisdiyanti, P., Hanafi, M., Tanaka, M., Sone, T., & Asano, K. (2011). Actinomycete Nonomuraea sp. isolated from Indonesian soil is a new producer of inulin fructotransferase. J. Biosciences and Bioeneering, 111, 671-674. https://doi.org/10.1016/j.jbiosc.2011.02.001
Pratiwi, I. Y. & Oki, K. (2019). Kandungan gizi, beta karoten dan antioksidan pada tepung pisang tongka langit (Musa troglodytarum L.). Agritech, 39(1), 48-53.
Roberfroid, M. B. (2007). Prebiotics: the concept revisited. Journal Nutr., 137, 830-837.
Rawat, K. M. (2015). Use of water properties in food technology: a global view. International Journal of Food Properties, 17, 1034–1054. https://doi.org/10.1080/10942912.2011.650339
Richana, N. (2019). Manfaat Umbi-umbian Indonesia. Penerbit Nuansa Cendikia. Bandung. Edisi cetakan II. ISBN 978-602-8394—90-1
Soaib, M., Shehzad, A., Omar, M., Rakha, A., Raza, H., Sharif, H. R. & Niazi, S. (2016). Inulin: properties, health benefits and food applications. Carbohydrate Polymers, 147, 444-454.
Singh, P. K. & Gill (2006). Production of inulinases: Recent advan ces, Food Technol. Biotechnol, 44, 151–162.
Saito, K. & Fusao, T. (2000). Difructose anhydrides: their mass-production and physiological functions. Biosci. Biotechnol.Biochem, 64(7), 1321-1327.
Suzuki, T., Hara, H., Kasai, T. & Tomita, F. (1998). Effects of difructose anhydride III on calcium absorption in small and large intestines of rats. Biosci Biotechnol Biochem, 62, 837–841.
Sabda, M., Wulaningtyas, H. S., Ondikeleuw, M., & Baliadi, Y. (2019). Karakterisasi potensi gembili (Dioscoreaesculenta L.) lokal asal Papua sebagai alternatif bahan pangan pokok. Buletin Plasma Nutfah, 25(1), 25–32.
Teferra, T. F. (2019). Direct and indirect actions of inulin as prebiotic polysaccharide: a review. CPQ Nutrition, 3, 6.
Vijayaraghavan, K., Ambika, D. Y. V., & Sowdamini, N. S. (2009). Trends in inulinase production – A review, Crit. Rev. Biotechnol, 29, 67–77. https://doi.org/ 10.1080/07388550802685389
Vilhalva, D. A. A., Cortelazzo, A. L., Carvalho, A. L. M. A. M., & Figueiredo-Ribeiro, R. D. C. L. (2011). Histochemistry and ultrastructure of campuloclinium chlorolepis (Asteraceae) tuberous roots accumulating fructan: evidences of functions other than reserve carbohydrate. Australian Journal of Botany, 59(1), 46–52. https://doi.org/10.1071/BT10126
Vergauwen, R., Laere, A. V., & Van., den Ende, W. V. (2003). Properties of fructan: fructan 1-fructosyltransferases from chicory and globe thistle, two asteracean plants storing greatly different types of inulin. Plant Physiology, 133, 391–401. Https://doi.org/10.1104/pp.103.026807
Wang, X. & Gibson, G. R. (1993). Effects on in vitro fermentation of oligofructose and inulin by bacteria growing in the human large instenstine. Journal of Applied Bacteriology, 75(4), 373-380.
Widowati, S., Sunarti., T. C. & Zaharani, A. (2005). Ekstraksi, karakterisasi dan kajian potensi prebiotik inulin dari umbi dahlia (Dahlia pinnata L). Seminar Rutin Puslitbang Tanaman Pangan. Bogor, 16 Juni 2005.
Waisnawi, P. A. G., Yusasrini, N. L. A., & Ina, P. T. (2019). Pengaruh perbandingan tepung suweg (Amorphophallus campanulatus) dan tepung kacang hijau (Vigna radiate) terhadap karakteristik cookies. Jurnal Ilmu dan Teknologi Pangan, 8(1), 48-56.
Winarti, S., Harmayai, E., & Nurismanto, R. (2011). Extraction of inulin from various yam tubers (Dioscorea spp). In 12th ASEAN Food Conference. Bangkok: BITEC.
Yanti, I. G., Miranda, A., Anni, F., & Budhi, O. (2019). Simple determination of inulin polymerization degree average from dahlia tubers using spectrofotometer. International Journal of Research and Review.
Yudhistira, B., Siswanti, & Luwidharto, J. C. N. (2020). The effect of solvent ratio and precipitation time on isolation of inulin from white sweet potato (Ipomoea batatas L.). The 5th International Seminar on Agribusiness 2019 IOP Conf. Series: Earth and Environmental Science 518. 012009 IOP Publishing. https://doi.org/10.1088/1755-1315/518/1/012009
DOI: https://doi.org/10.22146/agritech.68463
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