Skrining pada Berbagai Jenis Umbi dan Pisang sebagai Sumber Difructose Anhyride III (DFA III) Melalui Reaksi Enzimatis

https://doi.org/10.22146/agritech.68463

Diah Ratnaningrum(1*), Een Sri Endah(2), Sabarni Gaffar(3), Sri Pudjiraharti(4)

(1) Pusat Riset Lingkungan dan Teknologi Bersih-Badan Riset Inovasi dan Nasional (BRIN), Jl. Cisitu Sangkuriang, Bandung, Jawa Barat 40135
(2) Pusat Riset Lingkungan dan Teknologi Bersih-Badan Riset Inovasi dan Nasional (BRIN), Jl. Cisitu Sangkuriang, Bandung, Jawa Barat 40135
(3) Program Studi Diploma Analis Kimia, Fakultas Matematika dan Ilmu Pengetahuan Indonesia, Universitas Padjajaran, Kampus Jatinangor, Jl. Jatinangor, Sumedang 45360
(4) Pusat Riset Lingkungan dan Teknologi Bersih-Badan Riset Inovasi dan Nasional (BRIN), Jl. Cisitu Sangkuriang, Bandung, Jawa Barat 40135
(*) Corresponding Author

Abstract


Difructosa Anhyride III (DFA III) dapat diproduksi melalui reaksi enzimatis menggunakan inulin. Inulin dapat diperoleh dari akar/umbi dan batang tanaman. Pada penelitian ini, jenis umbi-umbian dan pisang yang diketahui sebagai salah satu sumber inulin dan akan diproses untuk pembuatan DFA III menggunakan inulin fructotransferase (IFTase) Nonomurae sp. Tujuan penelitian ini adalah melakukan tahapan skrining untuk memperoleh bahan baku sumber inulin dari berbagai jenis jenis umbi dan pisang yang berpotensi sebagai sumber pembuatan DFA III. Metode yang digunakan yaitu melalui reaksi enzimatis menggunakan Inulin fruktotransferase (IFTase). Umbi dan pisang diblansing, dipotong dan diblender dan ditambahkan air panas dengan rasio 1:2. Ekstrak inulin yang dihasilkan diukur secara kualitatif menggunakan metoda kromatografi lapis tipis (KLT) dan metode spektrofotometer. Hasil penelitian menujukkan bahwa umbi jalar mempunyai potensi besar dalam pembentukan DFA III dengan kadar inulin sebesar 7.03%, sedangkan untuk jenis pisang tidak berpotensi dalam pembentukan DFA III, hanya memiliki kandungan inulin saja (20,1%).


Keywords


DFA III; inulin, inulin fructotransferase; KLT; Nonomurae sp.

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DOI: https://doi.org/10.22146/agritech.68463

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