Fraksinasi Enzim Lipase dari Endosperm Kelapa dengan Metode Salting Out

https://doi.org/10.22146/agritech.9532

Moh. Su'i(1*), Suprihana Suprihana(2)

(1) Fakultas Pertanian, Universitas Widyagama Malang, Jl. Borobudur No. 25, Malang 65128
(2) Fakultas Pertanian, Universitas Widyagama Malang, Jl. Borobudur No. 25, Malang 65128
(*) Corresponding Author

Abstract


This research learns about fractionation of lipases activity from coconut endosperm by using ammonium sulphate of 0–15%; 15-30 %, 30–45 %, 45–60 %, 60–75 % and 75–90 %. The results showed that the fractions of 0–15% ; 30–45 %, 45–60 % and 60–75 % have lipase activity. Meanwhile, the highest activity was fractions of 60-75%. fractions of 15-30% and 75-90%  have no lipase enzym activity. Molecule weigh of lipase enzyme was 72 kDa.

ABSTRAK

Penelitian ini mempelajari fraksinasi enzim lipase dari endosperm kelapa menggunakan ammonium sulfat. fraksinasi dilakukan dengan variasi konsentrasi ammonium sulfat 0–15% ; 15-30%; 30–45 %, 45–60 %, 60–75 % dan 75–90 %. Hasil penelitian menunjukkan bahwa enzim lipase terdapat pada fraksi 0–15% ; 30–45 %, 45–60 % dan fraksi 60–75 % dengan aktivitas enzim tertinggi pada fraksi 60-75%. Sedangkan fraksi 15-30% dan 75-90% tidak ada enzim lipase. Berat molekul enzim lipase pada semua fraksi 72 kDa.


Keywords


Lipases; endosperm; coconut; fractionation; ammonium sulphate

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DOI: https://doi.org/10.22146/agritech.9532

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Agritech (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.


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