Analisis Kromatografi Cair Kinerja Tinggi untuk Penetapan Kadar Asam Galat, Kafein dan Epigalokatekin Galat pada Beberapa Produk Teh Celup

https://doi.org/10.22146/agritech.9578

Yohanes Martono(1*), Sudibyo Martono(2)

(1) Program Studi Kimia, Fakultas Sains dan Matematika, Universitas Kristen Satya Wacana, Gedung Y Jalan Diponegoro 52-60, Salatiga 50733
(2) Bagian Kimia Farmasi, Fakultas Farmasi, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(*) Corresponding Author

Abstract


A rapid and accurate High Performance Liquid Chromatography (HPLC) method for determination of gallic acid, caffein, and epigallocatechin gallate (EGCG) with an isocratic mobile phase elution was developed. HPLC parameters consisted of a C18 reversed-phase column (Eurosphere C18, 250 × 4.6 mm i.d., 5μm), mobile phase composed of 0.1% ortho phosporic acid : water : acetonitrile : methanol (14 : 7 : 3 : 1 v/v/v/v) pH = 4.00, flow rate 1.2 mL/min and was detected by UV detector at 280 nm. The validation of this method was confirmed from selectivity, linearity, accuracy and precision, limit of detection and limit of quantification. The method developed has proved to comply with validation requirements and has been applied to analyze various tea bag products (green and black tea). In green tea, the results showed that the highest amount of constituent was EGCG followed by caffein and gallic acid, whereas in black tea, EGCG can not be measured.

 

ABSTRAK

Metode Kromatografi Cair Kinerja Tinggi (KCKT) yang cepat dan akurat untuk penetapan kadar asam galat, kafein dan epigalokatekin galat (EGCG) dengan sistem elusi fase gerak secara isokratik telah dikembangkan. Sistem KCKT terdiri atas kolom fase terbalik C18 (Eurosphere C18, 250 × 4,6 mm i.d., 5μm), fase gerak campuran asam orto-fosfat 0,1% : air : asetonitril : metanol (14 : 7 : 3 : 1 v/v/v/v) pH = 4,00 dengan kecepatan alir 1,2 mL/min serta dideteksi pada UV 280 nm. Validasi metode ini dikonfirmasi terhadap selektivitas, linearitas, akurasi, presisi, batas deteksi dan batas kuantitasi. Metode yang dikembangkan dapat memenuhi syarat-syarat validasi metode dan telah digunakan untuk menganalisis berbagai produk teh celup (teh hijau dan hitam). Pada teh hijau, hasilnya menunjukkan bahwa kandungan terbesar adalah EGCG diikuti kafein dan asam galat sedangkan pada teh hitam, EGCG tidak terkuantitasi.


Keywords


HPLC isocratic; gallic acid; caffein; epigallocatechin gallate

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DOI: https://doi.org/10.22146/agritech.9578

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agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.


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