Enhancing the Quality of Ready-to-Drink Robusta Coffee through Enzymatic Decaffeination, Cold Brew Techniques, and Sterilization

https://doi.org/10.22146/agritech.103822

Rahma Widya Puspita(1), Chusnul Hidayat(2*), Supriyadi Supriyadi(3)

(1) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281
(2) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281
(3) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281; Center of Excellence for Indonesian Gastronomy, Universitas Gadjah Mada, Jl. Flora No 1 Bulaksumur, Yogyakarta 55281
(*) Corresponding Author

Abstract


Cold brew coffee has been reported to possess distinct sensory characteristics compared to hot brew. Therefore, this study aims to enhance the flavor of decaffeinated robusta cold brew coffee as a specialty coffee. The decaffeination process (using bromelain enzyme from pineapple), cold brew (immersion method), and sterilization techniques were used to examine the sensory qualities and physicochemical characteristics (color, pH, TDS, caffeine, chlorogenic acid/CGA, trigonelline, and volatile compounds) of decaffeinated robusta cold brew coffee and ready-to-drink decaffeinated robusta cold brew coffee. Medium-roasted robusta coffee was used in 2 different brewing methods, namely cold brew (T= 15 °C, 20 °C, 25 °C; t= 8, 10, 12 h) and hot brew as a control. The sensory analysis was conducted by trained panelists from Indonesian Coffee and Cocoa Research Institute using cupping technique. The Specialty Coffee American Association (SCAA) method was used to determine the flavor profile of a fine robusta cupping form.The results showed that the highest overall sensory score was obtained by decaffeinated cold brew robusta at 20°C for 8 hours. It contained 0.081 mg/100 g caffeine, 0.024 mg/100 g CGA, and 0.048 mg/100 g trigonellineSpecialty coffee could be defined as ready-to-drink cold brew coffee brewed at 20 °C for 10 hours, 25 °C for 8 hours, and 25 °C for 10 hours. Notes, such as brown sugar, honey, caramel, chocolate, and herbal, were enhanced by sterilization, while hot brew coffee presented a less favorable profile, scoring only 78.25.

Keywords


Robusta; decaffeinated coffee; cold brew; sterilization; specialty coffee

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References

Acquaticci, L., Angeloni, S., Cela, N., Galgano, F., Vittori, S., Caprioli, G., & Condelli, N. (2023). Impact of coffee species, post-harvesting treatments and roasting conditions on coffee quality and safety related compounds. Food Control, 149, 109714. https://doi.org/10.1016/j.foodcont.2023.109714

Afriliana, A., Harada, H., Khotijah, P. Q., Jayus, -, & Giyarto, -. (2018). Fermented Technology of Robusta Coffee Beans (Canephora Coffee) With Kefir Milk to Produce Specialty Coffee. Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018). Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018), Yogyakarta, Indonesia. https://doi.org/10.2991/fanres-18.2018.61

Angeloni, G., Guerrini, L., Masella, P., Bellumori, M., Daluiso, S., Parenti, A., & Innocenti, M. (2019). What kind of coffee do you drink? An investigation on effects of eight different extraction methods. Food Research International, 116, 1327–1335. https://doi.org/10.1016/j.foodres.2018.10.022

Awwad, S., Issa, R., Alnsour, L., Albals, D., & Al-Momani, I. (2021). Quantification of Caffeine and Chlorogenic Acid in Green and Roasted Coffee Samples Using HPLC-DAD and Evaluation of the Effect of Degree of Roasting on Their Levels. Molecules, 26(24), 7502. https://doi.org/10.3390/molecules26247502

Badmos, S., Lee, S.-H., & Kuhnert, N. (2019). Comparison and quantification of chlorogenic acids for differentiation of green Robusta and Arabica coffee beans. Food Research International, 126, 108544. https://doi.org/10.1016/j.foodres.2019.108544

Baggenstoss, J., Poisson, L., Kaegi, R., Perren, R., & Escher, F. (2008). Coffee Roasting and Aroma Formation: Application of Different Time−Temperature Conditions. Journal of Agricultural and Food Chemistry, 56(14), 5836–5846. https://doi.org/10.1021/jf800327j

Baqueta, M. R., Diniz, P. H. G. D., Pereira, L. L., Almeida, F. L. C., Valderrama, P., & Pallone, J. A. L. (2024). An overview on the Brazilian Coffea canephora scenario and the current chemometrics-based spectroscopic research. Food Research International, 194, 114866. https://doi.org/10.1016/j.foodres.2024.114866

Bellumori, M., Angeloni, G., Guerrini, L., Masella, P., Calamai, L., Mulinacci, N., Parenti, A., & Innocenti, M. (2021). Effects of different stabilization techniques on the shelf life of cold brew coffee: Chemical composition, flavor profile and microbiological analysis. LWT, 142, 111043. https://doi.org/10.1016/j.lwt.2021.111043

Bressanello, D., Liberto, E., Cordero, C., Rubiolo, P., Pellegrino, G., Ruosi, M. R., & Bicchi, C. (2017). Coffee aroma: Chemometric comparison of the chemical information provided by three different samplings combined with GC–MS to describe the sensory properties in cup. Food Chemistry, 214, 218–226. https://doi.org/10.1016/j.foodchem.2016.07.088

Cai, Y., Xu, Z., Pan, X., Gao, M., Wu, M., Wu, J., & Lao, F. (2022). Comparative Profiling of Hot and Cold Brew Coffee Flavor Using Chromatographic and Sensory Approaches. Foods, 11(19), 2968. https://doi.org/10.3390/foods11192968

Caporaso, N., Whitworth, M. B., & Fisk, I. D. (2022). Prediction of coffee aroma from single roasted coffee beans by hyperspectral imaging. Food Chemistry, 371, 131159. https://doi.org/10.1016/j.foodchem.2021.131159

Chiralertpong, A., Acree, T. E., Barnard, J., & Siebert, K. J. (2008). Taste–Odor Integration in Espresso Coffee. Chemosensory Perception, 1(2), 147–152. https://doi.org/10.1007/s12078-008-9018-0

Cordoba, N. (2020). Coffee extraction_ A review of parameters and their influence on the physicochemical characteristics and flavour of coffee brews.

Cordoba, N., Fernandez-Alduenda, M., Moreno, F. L., & Ruiz, Yolanda. (2020). Coffee extraction: A review of parameters and their influence on the physicochemical characteristics and flavour of coffee brews. Trends in Food Science & Technology, 96, 45–60. https://doi.org/10.1016/j.tifs.2019.12.004

Córdoba, N., Moreno, F. L., Osorio, C., Velásquez, S., Fernandez-Alduenda, M., & Ruiz-Pardo, Y. (2021). Specialty and regular coffee bean quality for cold and hot brewing: Evaluation of sensory profile and physicochemical characteristics. LWT, 145, 111363. https://doi.org/10.1016/j.lwt.2021.111363

Cordoba, N., Pataquiva, L., Osorio, C., Moreno, F. L. M., & Ruiz, R. Y. (2019). Effect of grinding, extraction time and type of coffee on the physicochemical and flavour characteristics of cold brew coffee. Scientific Reports, 9(1), 8440. https://doi.org/10.1038/s41598-019-44886-w

Cui, D. D., Liu, Y., Chen, Y. P., Feng, X., Lu, Y., & Yu, B. (2020). Application of SPME‐GC‐TOFMS, E‐nose, and sensory evaluation to investigate the flavor characteristics of Chinese Yunnan coffee at three different conditions (beans, ground powder, and brewed coffee). Flavour and Fragrance Journal, 35(5), 541–560. https://doi.org/10.1002/ffj.3597

Darmajana, D. A., Wulandari, R., & Sagita, D. (2022). Characteristics of cold brewed arabica coffee from Gunung Karamat Village Subang Regency as a result of different temperatures and roasting time. INMATEH Agricultural Engineering, 835–843. https://doi.org/10.35633/inmateh-68-83

Dawidowicz, A. L., & Typek, R. (2017). Transformation of chlorogenic acids during the coffee beans roasting process. European Food Research and Technology, 243(3), 379–390. https://doi.org/10.1007/s00217-016-2751-8

Deák, T. (2014). Food Technologies: Sterilization. In Encyclopedia of Food Safety (pp. 245–252). Elsevier. https://doi.org/10.1016/B978-0-12-378612-8.00258-4

Dooley, L., Lee, Y., & Meullenet, J.-F. (2010). The application of check-all-that-apply (CATA) consumer profiling to preference mapping of vanilla ice cream and its comparison to classical external preference mapping. Food Quality and Preference, 21(4), 394–401. https://doi.org/10.1016/j.foodqual.2009.10.002

Farah, A. (2012). Coffee Constituents. In Y. Chu (Ed.), Coffee (1st ed., pp. 21–58). Wiley. https://doi.org/10.1002/9781119949893.ch2

Fibrianto, K., Umam, K., & Shinta Wulandari, E. (2018). Effect of Roasting Profiles and Brewing Methods on the Characteristics of Bali Kintamani Coffee. Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018). Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018), Yogyakarta, Indonesia. https://doi.org/10.2991/fanres-18.2018.40

Fitzhugh, D. J., Shan, S., Dewhirst, M. W., & Hale, L. P. (2008). Bromelain treatment decreases neutrophil migration to sites of inflammation. Clinical Immunology, 128(1), 66–74. https://doi.org/10.1016/j.clim.2008.02.015

Fuller, M., & Rao, N. Z. (2017). The Effect of Time, Roasting Temperature, and Grind Size on Caffeine and Chlorogenic Acid Concentrations in Cold Brew Coffee. Scientific Reports, 7(1), 17979. https://doi.org/10.1038/s41598-017-18247-4

Ghani, A. G. A., Farid, M. M., Chen, X. D., & Richards, P. (2001). Thermal sterilization of canned food in a 3-D pouch using computational ¯uid dynamics. Journal of Food Engineering.

Gloess, A. N., Vietri, A., Wieland, F., Smrke, S., Schönbächler, B., López, J. A. S., Petrozzi, S., Bongers, S., Koziorowski, T., & Yeretzian, C. (2014). Evidence of different flavour formation dynamics by roasting coffee from different origins: On-line analysis with PTR-ToF-MS. International Journal of Mass Spectrometry, 365–366, 324–337. https://doi.org/10.1016/j.ijms.2014.02.010

Hapsari, A. R., Broto, R. T. W., & Apriyanti, E. (2022). Pemanfaatan Enzim Bromelin dari Tepung Nanas (Ananas comosus L. Merr) untuk Menurunkan Kadar Kafein Kopi pada Proses Pembuatan Sirup Kopi. METANA, 18(1), 57–64. https://doi.org/10.14710/metana.v18i1.45600

Heinz, G., & Hautzinger, P. (2007). Meat processing technology for small- to medium-scale producers. Food and Agriculture Organizations of the United Nations, Regional Office for Asia and the Pacific.

Heo, J., Adhikari, K., Choi, K. S., & Lee, J. (2020). Analysis of Caffeine, Chlorogenic Acid, Trigonelline, and Volatile Compounds in Cold Brew Coffee Using High-Performance Liquid Chromatography and Solid-Phase Microextraction—Gas Chromatography-Mass Spectrometry. Foods, 9(12), 1746. https://doi.org/10.3390/foods9121746

International Coffee Organization (ICO). (2023). Coffee Report and Outlook. Accessed from https://ico.org/

Kalaska, B., Piotrowski, L., Leszczynska, A., Michalowski, B., Kramkowski, K., Kaminski, T., Adamus, J., Marcinek, A., Gebicki, J., Mogielnicki, A., & Buczko, W. (2014). Antithrombotic Effects of Pyridinium Compounds Formed from Trigonelline upon Coffee Roasting. Journal of Agricultural and Food Chemistry, 62(13), 2853–2860. https://doi.org/10.1021/jf5008538

Kusumaningrum, A., Nurhikmat, A., Praharasti, A. S., Susanto, A., Arthatiani, F. Y., & Zulham, A. (2017). Study of lethality Value and Chemical Characteristics of “Keumamah-Processed Cuisine” for Development of Small and Medium Enterprise Product. Jurnal Riset Teknologi Industri, 11(2), 83–90. https://doi.org/10.26578/jrti.v11i2.2910

Liang, J., Batali, M. E., Routt, C., Ristenpart, W. D., & Guinard, J.-X. (2024). Sensory analysis of the flavor profile of full immersion hot, room temperature, and cold brewed coffee over time. Scientific Reports, 14(1), 19298. https://doi.org/10.1038/s41598-024-69867-6

Lingle, T. R., & Menon, S. N. (2017). Cupping and Grading—Discovering Character and Quality. In The Craft and Science of Coffee (pp. 181–203). Elsevier. https://doi.org/10.1016/B978-0-12-803520-7.00008-6

Liu, C., Yang, N., Yang, Q., Ayed, C., Linforth, R., & Fisk, I. D. (2019). Enhancing Robusta coffee aroma by modifying flavour precursors in the green coffee bean. Food Chemistry, 281, 8–17. https://doi.org/10.1016/j.foodchem.2018.12.080

Ludwig, I. A., Sanchez, L., Caemmerer, B., Kroh, L. W., De Peña, M. P., & Cid, C. (2012). Extraction of coffee antioxidants: Impact of brewing time and method. Food Research International, 48(1), 57–64. https://doi.org/10.1016/j.foodres.2012.02.023

M.R.N. Alcantara, G., Dresch, D., & R. Melchert, W. (2021). Use of non-volatile compounds for the classification of specialty and traditional Brazilian coffees using principal component analysis. Food Chemistry, 360, 130088. https://doi.org/10.1016/j.foodchem.2021.130088

Moreira, A. S. P., Nunes, F. M., Domingues, M. R., & Coimbra, M. A. (2012). Coffee melanoidins: Structures, mechanisms of formation and potential health impacts. Food & Function, 3(9), 903. https://doi.org/10.1039/c2fo30048f

Murda, Y. K., Husni, A., Budhiyanti, S. A., & Novita, E. R. (2016). Karakteristik Kimia dan Mikrobiologi Filet Lele Dumbo Asap Berbumbu dalam Kaleng. 19.

Narváez, E., Zapata, E., Dereix, J. D., Lopez, C., Torijano-Gutiérrez, S., & Zapata, J. (2024). A Comparative Analysis of Cold Brew Coffee Aroma Using the Gas Chromatography–Olfactometry–Mass Spectrometry Technique: Headspace–Solid-Phase Extraction and Headspace Solid-Phase Microextraction Methods for the Extraction of Sensory-Active Compounds. Molecules, 29(16), 3791. https://doi.org/10.3390/molecules29163791

Ogawa, A., & Cho, H. (2015). Role of food emulsifiers in milk coffee beverages. Journal of Colloid and Interface Science, 449, 198–204. https://doi.org/10.1016/j.jcis.2015.01.063

Palupi, N. S. & Fatimah. (2021). The Role of Decaffeinated Coffee in Reducing the Risk of Hypertension: A Systematic Review. Journal of Functional Food and Nutraceutical, 99–116. https://doi.org/10.33555/jffn.v2i2.60

Park, H., Noh, E., Kim, M., & Lee, K.-G. (2024). Analysis of volatile and nonvolatile compounds in decaffeinated and regular coffee prepared under various roasting conditions. Food Chemistry, 435, 137543. https://doi.org/10.1016/j.foodchem.2023.137543

Park, J. B., Peters, R., & Novotny, J. A. (2023). Impact of roasting on javamide-I/-II in Arabica and Robusta coffee beans. Food Chemistry, 412, 135586. https://doi.org/10.1016/j.foodchem.2023.135586

Portela, C. D. S., Almeida, I. F. D., Mori, A. L. B., Yamashita, F., & Benassi, M. D. T. (2021). Brewing conditions impact on the composition and characteristics of cold brew Arabica and Robusta coffee beverages. LWT, 143, 111090. https://doi.org/10.1016/j.lwt.2021.111090

Pramadika, R. P., Kusnadi, J., & Wijayanti, S.D. (2016). Dekafeinasi Kopi Robusta Enzimatis (Coffee canephora L.) Dengan Enzim Bromelin Kasar dari Ekstrak Buah Nanas (Annanas Comosus Var Comosus). Jurnal Pangan Dan Agroindustri, 4(4)

Ramalakshmi, K., & Raghavan, B. (1999). Caffeine in Coffee: Its Removal. Why and How? Critical Reviews in Food Science and Nutrition, 39(5), 441–456. https://doi.org/10.1080/10408699991279231

Ramírez-Correa, P., Rondán-Cataluña, F. J., Moulaz, M. T., & Arenas-Gaitán, J. (2020). Purchase Intention of Specialty Coffee. Sustainability, 12(4), 1329. https://doi.org/10.3390/su12041329

Rao, N. Z., & Fuller, M. (2018). Acidity and Antioxidant Activity of Cold Brew Coffee. Scientific Reports, 8(1), 16030. https://doi.org/10.1038/s41598-018-34392-w

Santanatoglia, A., Angeloni, S., Fiorito, M., Fioretti, L., Ricciutelli, M., Sagratini, G., Vittori, S., & Caprioli, G. (2024). Development of new analytical methods for the quantification of organic acids, chlorogenic acids and caffeine in espresso coffee by using solid-phase extraction (SPE) and high-performance liquid chromatography-diode array detector (HPLC-DAD). Journal of Food Composition and Analysis, 125, 105732. https://doi.org/10.1016/j.jfca.2023.105732

Secretary General Ministry of Agriculture. (2023). Coffee Plantation Commodities. Jakarta: Secretary General Ministry of Agriculture of the Republic of Indonesia, 2023

Servín-Juárez, R., Trejo-Pech, C. J. O., Pérez-Vásquez, A. Y., & Reyes-Duarte, Á. (2021). Specialty Coffee Shops in Mexico: Factors Influencing the Likelihood of Purchasing High-Quality Coffee. Sustainability, 13(7), 3804. https://doi.org/10.3390/su13073804

Specialty Coffee Association of America (SCAA). (2015). “SCAA Protocols: Cupping Specialty Coffee”. America: The Specialty Coffee Association of America, 2015

Suryaningsih, W., Bakri, A., Kautsar, S., Hariono, B., Brilliantina, A., & Wijaya, R. (2022). Prototype of Integrated Mini Exhausting System For Fish Canning Process. IOP Conference Series: Earth and Environmental Science, 980(1), 012039. https://doi.org/10.1088/1755-1315/980/1/012039

Teixeira, A. A. (2014). Thermal Food Preservation Techniques (Pasteurization, Sterilization, Canning and Blanching). In S. Bhattacharya (Ed.), Conventional and Advanced Food Processing Technologies (1st ed., pp. 115–128). Wiley. https://doi.org/10.1002/9781118406281.ch6

Wang, W. (2020). Effect of sterilization and storage on volatile compounds, sensory properties and physicochemical properties of coconut milk. Microchemical Journal.

Wang, W., Chen, H., Ke, D., Chen, W., Zhong, Q., Chen, W., & Yun, Y.-H. (2020). Effect of sterilization and storage on volatile compounds, sensory properties and physicochemical properties of coconut milk. Microchemical Journal, 153, 104532. https://doi.org/10.1016/j.microc.2019.104532

Wang, X., & Lim, L. (2021). Modeling study of coffee extraction at different temperature and grind size conditions to better understand the cold and hot brewing process. Journal of Food Process Engineering, 44(8), e13748. https://doi.org/10.1111/jfpe.13748

Yeager, S. E., Batali, M. E., Lim, L. X., Liang, J., Han, J., Thompson, A. N., Guinard, J., & Ristenpart, W. D. (2022). Roast level and brew temperature significantly affect the color of brewed coffee. Journal of Food Science, 87(4), 1837–1850. https://doi.org/10.1111/1750-3841.16089

Zhang, Z., Hu, G., Caballero, B., Appel, L., & Chen, L. (2011). Habitual coffee consumption and risk of hypertension: A systematic review and meta-analysis of prospective observational studies. The American Journal of Clinical Nutrition, 93(6), 1212–1219. https://doi.org/10.3945/ajcn.110.004044



DOI: https://doi.org/10.22146/agritech.103822

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